{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T20:26:21Z","timestamp":1787775981883,"version":"build-2784847793"},"reference-count":25,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background &amp; objectives:<\/jats:title>\n                    <jats:p>Theileriosis is an important tick-bome hemoprotozoan disease of cattle which causes severe economic loss due to morbidity and mortality. A diagnostic test having high sensitivity, specificity and easy application at the field level is the need of the hour. In this regard Loop-mediated isothermal amplification (LAMP) is proven to be a sensitive, easy and time efficient method. One of the major obstacles for the application of LAMP is the difficulty in maintaining the cold chain to preserve reagents. Thus, the challenge is to develop a LAMP kit in a ready-to-use format with dried reagents useful for quick and simple application in field conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>The optimized reaction of wet LAMP was followed for the standardization of dry LAMP with certain modifications which are needful. The major modification is vitrification technology of enzyme using trehalose.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>LAMP assay (dry and wet LAMP) was found to be more sensitive (100%) when compared to microscopy (69.5%) and PCR (86.9%). It was observed that the dry LAMP reaction tubes at room temperature as well as refrigeration temperature provided successful amplification till 7 weeks.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Interpretation &amp; conclusion:<\/jats:title>\n                    <jats:p>The drying conditions of LAMP reagents were optimized, and finally managed to dry them in a single reaction tube without reducing the sensitivity. This technology enables us to transport LAMP kits to areas where the cold chain is not easily available.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/0972-9062.392261","type":"journal-article","created":{"date-parts":[[2024,1,2]],"date-time":"2024-01-02T03:00:14Z","timestamp":1704164414000},"page":"357-363","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["Dry LAMP: A point of care diagnostics for diagnosis of bovine tropical theileriosis"],"prefix":"10.4103","volume":"61","author":[{"given":"Sindhoora","family":"Divakar","sequence":"first","affiliation":[{"name":"Department of Veterinary Parasitology, Veterinary College, Bengaluru, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Dhanalakshmi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Parasitology, Veterinary College, Bengaluru, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"Sandeep","sequence":"additional","affiliation":[{"name":"Department of Veterinary Parasitology, Veterinary College, Bengaluru, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shrikrishna","family":"Isloor","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology, Veterinary College, Bengaluru, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Rashmi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pharmacology, Veterinary College, Bengaluru, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KJ","family":"Ananda","sequence":"additional","affiliation":[{"name":"Department of Veterinary Parasitology, Veterinary College, Shimogga, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manjunatha","family":"Reddy","sequence":"additional","affiliation":[{"name":"National Institute of Veterinary Epidemiology and Disease Informatics, Yelhanka, Bengaluru, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2024,9,21]]},"reference":[{"key":"R1-20240921","first-page":"32","article-title":"Prevalence of tickborne diseases in Punjab (Pakistan) and hematological profile of Anaplasma marginale infection in indigenous and crossbred cattle","volume":"64","author":"Atif","year":"2012","journal-title":"Pak J Sci"},{"issue":"2","key":"R2-20240921","doi-asserted-by":"crossref","first-page":"151","DOI":"10.14202\/vetworld.2018.151-160","article-title":"Projected economic losses due to vector and vector-borne parasitic diseases in livestock of India and its significance in implementing the concept of integrated practices for vector management","volume":"11","author":"Narladkar","year":"2018","journal-title":"Vet World"},{"issue":"6","key":"R3-20240921","first-page":"46","article-title":"Recent developments in diagnostics of some tick-borne diseases. 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