{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:41:20Z","timestamp":1786650080498,"version":"build-2736575974"},"reference-count":21,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>To explore the correlation between ROBO2 and RASSF2A gene methylations and gastric cancer family history.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>ROBO2 and RASSF2A gene methylations in gastric cancer tissues and peri.cancerous tissues were detected with methylation.specific PCR in 36. patients with gastric cancer family history and 33 without gastric cancer family history. The correlations of ROBO2 and RASSF2A gene methylations with family history, and clinical and pathological characteristics were analyzed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      ROBO2 and RASSF2A gene methylations were all significantly higher in gastric cancer tissues (30% and 26%) than in peri-cancerous tissues (0% and 0%) (all\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.05). ROBO2 gene methylation was significantly lower in the patients with gastric cancer family history (17%, 6\/36) than in the patients without gastric cancer family history (41%, 15\/33) (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>ROBO2 and RASSF2A gene methylations may be related to gastric tumorigenesis, and ROBO2 gene methylation is associated with sporadic gastric cancer.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/0973-1482.146089","type":"journal-article","created":{"date-parts":[[2016,7,26]],"date-time":"2016-07-26T05:22:30Z","timestamp":1469510550000},"page":"597-600","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":9,"title":["Correlations between gastric cancer family history and ROBO2 and RASSF2A gene methylations"],"prefix":"10.4103","volume":"12","author":[{"given":"Zhi-Wei","family":"Chang","sequence":"first","affiliation":[{"name":"Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Ru","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai-Yun","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Pathology, Linzhou Center Hospital, Linzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing-Li","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Linzhou Center Hospital, Linzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2016,7,25]]},"reference":[{"key":"R1-20260109","first-page":"69","article-title":"Global cancer statistics","volume":"61","author":"Jemal","year":"2011","journal-title":"CA Cancer J Clin"},{"key":"R2-20260109","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1093\/ije\/dyg152","article-title":"Aggregation of stomach cancer history in parents and offspring in comparison with other sites","volume":"32","author":"Kondo","year":"2003","journal-title":"Int J Epidemiol"},{"key":"R3-20260109","doi-asserted-by":"crossref","first-page":"2l","DOI":"10.1177\/106689690601400105","article-title":"Genetics, pathology, and clinics of familial gastric cancer","volume":"14","author":"Oliveira","year":"2006","journal-title":"Int J Surg Pathol"},{"key":"R4-20260109","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/s00439-008-0610-9","article-title":"Alterations of ROBO1\/DUTT1 and ROBO2 loci in early dysplastic lesions of head and neck: Clinical and prognostic implications","volume":"125","author":"Ghosh","year":"2009","journal-title":"Hum Genet"},{"key":"R5-20260109","doi-asserted-by":"crossref","first-page":"28045","DOI":"10.1074\/jbc.M300554200","article-title":"RASSF2 is a novel K-Ras-pecific effector and potential tumor suppressor","volume":"278","author":"Vos","year":"2003","journal-title":"J Biol Chem"},{"key":"R6-20260109","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1093\/jnci\/92.21.1781","article-title":"Report on the first meeting of the International Collaborative Group on Hereditary Gastric Cancer","volume":"92","author":"Park","year":"2000","journal-title":"J Natl Cancer Inst"},{"key":"R7-20260109","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/S0002-9440(10)65157-7","article-title":"Methylation status of the Epstein-Barr virus major latent promoter C in iatrogenic B cell lymphoproliferative disease: Application of PCR-based analysis","volume":"155","author":"Tao","year":"1999","journal-title":"Am J Pathol"},{"key":"R8-20260109","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1158\/1535-7163.MCT-08-0860","article-title":"Epigenetics in cancer: Targeting chromatin modifications","volume":"8","author":"Ellis","year":"2009","journal-title":"Mol Cancer Ther"},{"key":"R9-20260109","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1016\/j.febslet.2009.04.012","article-title":"CpG islands\u2014'a rough guide'","volume":"583","author":"Illingworth","year":"2009","journal-title":"FEBS Lett"},{"key":"R10-20260109","doi-asserted-by":"crossref","first-page":"9115","DOI":"10.1074\/jbc.M308083200","article-title":"Slit protein-mediated inhibition of CXCR4-induced chemotactic and chemoinvasive signaling pathways in breast cancer cells","volume":"279","author":"Prasad","year":"2004","journal-title":"J Biol Chem"},{"key":"R11-20260109","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1007\/s12253-013-9722-1","article-title":"Down-regulation of ROBO2 expression in prostate cancers","volume":"20","author":"Choi","year":"2014","journal-title":"Pathol Oncol Res"},{"key":"R12-20260109","first-page":"645","article-title":"Frameshift mutations of axon guidance genes ROBO1 and ROBO2 in gastric and colorectal cancers with microsatellite instability","volume":"45","author":"Je","year":"2013","journal-title":"Pathology"},{"key":"R13-20260109","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1053\/j.gastro.2005.03.051","article-title":"The Ras effector RASSF2 is a novel tumor-suppressor gene in human colorectal cancer","volume":"129","author":"Akino","year":"2005","journal-title":"Gastroenterology"},{"key":"R14-20260109","doi-asserted-by":"crossref","first-page":"3987","DOI":"10.1038\/sj.onc.1208566","article-title":"CpG island promoter hypermethylation of a novel Ras-effector gene RASSF2A is an early event in colon carcinogenesis and correlates inversely with K-ras mutations","volume":"24","author":"Hesson","year":"2005","journal-title":"Oncogene"},{"key":"R15-20260109","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1002\/ijc.22276","article-title":"Correlation between hypermethylation of the RASSF2A promoter and K-ras\/BRAF mutations in microsatellite-stable colorectal cancers","volume":"120","author":"Park","year":"2007","journal-title":"Int J Cancer"},{"key":"R16-20260109","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1093\/carcin\/bgn060","article-title":"Cytoplasmic RASSF2A is a proapoptotic mediator whose expression is epigenetically silenced in gastric cancer","volume":"29","author":"Maruyama","year":"2008","journal-title":"Carcinogenesis"},{"key":"R17-20260109","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1002\/ijc.22185","article-title":"Inactivation of RASSF2A by promoter methylation correlates with lymph node metastasis in nasopharyngeal carcinoma","volume":"120","author":"Zhang","year":"2007","journal-title":"Int J Cancer"},{"key":"R18-20260109","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1097\/MPA.0b013e318223d1a5","article-title":"Aberrant methylation of RASSF2A in human pancreatic ductal adenocarcinoma and its relation to clinicopathologic feature","volume":"41","author":"Zhao","year":"2012","journal-title":"Pancreas"},{"key":"R19-20260109","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1038\/sj.onc.1204234","article-title":"E-cadherin gene (CDH1) promoter methylation as the second hit in sporadic diffuse gastric carcinoma","volume":"20","author":"Machado","year":"2001","journal-title":"Oncogene"},{"key":"R20-20260109","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1097\/00019606-200603000-00004","article-title":"CDH1 gene promoter hypermethylation in gastric cancer: Relationship to Goseki grading, microsatellite instability status, and EBV invasion","volume":"15","author":"Zazula","year":"2006","journal-title":"Diagn Mol Pathol"},{"key":"R21-20260109","doi-asserted-by":"crossref","first-page":"4128","DOI":"10.1038\/sj.onc.1206651","article-title":"Age-related methylation of tumor suppressor and tumor-related genes: An analysis of autopsy samples","volume":"22","author":"Waki","year":"2003","journal-title":"Oncogene"}],"container-title":["Journal of Cancer Research and Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/01363817-201612020-00027","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:01:50Z","timestamp":1786647710000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/01363817-201612020-00027"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4]]},"references-count":21,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016]]}},"URL":"https:\/\/doi.org\/10.4103\/0973-1482.146089","relation":{},"ISSN":["0973-1482","1998-4138"],"issn-type":[{"value":"0973-1482","type":"print"},{"value":"1998-4138","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,4]]}}}