{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T14:32:27Z","timestamp":1786717947292,"version":"build-2736575974"},"reference-count":19,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Hum Reprod Sci"],"published-print":{"date-parts":[[2016]]},"DOI":"10.4103\/0974-1208.192070","type":"journal-article","created":{"date-parts":[[2016,10,12]],"date-time":"2016-10-12T07:06:16Z","timestamp":1476255976000},"page":"194","source":"Crossref","is-referenced-by-count":18,"title":["Ficoll-400 density gradient method as an effective sperm preparation technique for assisted reproductive techniques"],"prefix":"10.4103","volume":"9","author":[{"given":"HyacinthN","family":"Highland","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ASharma","family":"Rishika","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SShaikh","family":"Almira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PBansal","family":"Kanthi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"key-10.4103\/0974-1208.192070-1","first-page":"197","volume-title":"Dynamics of the mammalian sperm plasma membrane in the process of fertilization","author":"Flesch","year":"2000","journal-title":"Biochim Biophys Acta"},{"key":"key-10.4103\/0974-1208.192070-2","first-page":"41","volume-title":"Spermatozoa as biomarkers for the assessment of human male infertility and genotoxicity","author":"Anton","year":"2012","journal-title":"Syst Biol Reprod Med"},{"key":"key-10.4103\/0974-1208.192070-3","doi-asserted-by":"crossref","unstructured":"Bjorndahl L, Mortimer D, Barratt CL, Castilla JA, Menkveld R, Kvist U. Sperm Preparation. A Practical Guide to Basic Laboratory Andrology. USA: Cambridge University; 2010. p. 167-87.","DOI":"10.1017\/CBO9780511729942.007"},{"key":"key-10.4103\/0974-1208.192070-4","first-page":"357","volume-title":"Oxidative stress & male infertility","author":"Makker","year":"2009","journal-title":"Indian J Med Res","ISSN":"https:\/\/id.crossref.org\/issn\/0971-5916","issn-type":"print"},{"key":"key-10.4103\/0974-1208.192070-5","doi-asserted-by":"crossref","unstructured":"Beydola T, Sharma RK, Lee W, Agarwal A. Sperm preparation and selection techniques. In: Rizk B, Aziz N, Agarwal A, editors. Male Infertility Practice. New Delhi: Jaypee Brothers Medical Publishers; 2013. p. 244-51.","DOI":"10.5005\/jp\/books\/11840_29"},{"key":"key-10.4103\/0974-1208.192070-6","unstructured":"World Health Organization. WHO Laboratory Manual for the Examination and Processing of Human Semen. 5 th ed. WHO Press, Geneva: World Health Organization; 2010."},{"key":"key-10.4103\/0974-1208.192070-7","doi-asserted-by":"crossref","unstructured":"Mahadevan M, Baker G. Assessment and preparation of semen for in vitro fertilization. In: Wood C, Trounson A, editors. Clinical In vitro Fertilization. Berlin: Springer; 1984. p. 83-97.","DOI":"10.1007\/978-1-4471-3317-9_7"},{"key":"key-10.4103\/0974-1208.192070-8","doi-asserted-by":"crossref","unstructured":"Mortimer D. Sperm washing. In: Practical Laboratory Andrology. 1 st ed. USA: Oxford University Press; 1994. p. 267-86.","DOI":"10.1093\/oso\/9780195065954.003.0012"},{"key":"key-10.4103\/0974-1208.192070-9","first-page":"81","volume-title":"Bovine serum albumin, sperm motility, and the dilution effect","author":"Harrison","year":"1982","journal-title":"J Exp Zool"},{"key":"key-10.4103\/0974-1208.192070-10","first-page":"125","volume-title":"Ficoll gradient isolation of immature sperm of high purity and intactness from goat epididymis","author":"Haldar","year":"1990","journal-title":"Arch Androl"},{"key":"key-10.4103\/0974-1208.192070-11","first-page":"201","volume-title":"A triple-stain technique for evaluating normal acrosome reactions of human sperm","author":"Talbot","year":"1981","journal-title":"J Exp Zool"},{"key":"key-10.4103\/0974-1208.192070-12","doi-asserted-by":"crossref","unstructured":"Terquem A, Dadoune JP. Aniline blue staining of human spermatozoon chromatin: Evaluation of nuclear maturation. In: The Sperm Cell. J Andre, editor. The Hague: Martinus Nijhoff Publications; 1982. p. 249-52.","DOI":"10.1007\/978-94-009-7675-7_46"},{"key":"key-10.4103\/0974-1208.192070-13","first-page":"847","volume-title":"Influence of analytical and biological variation on the clinical interpretation of seminal parameters","author":"Castilla","year":"2006","journal-title":"Hum Reprod"},{"key":"key-10.4103\/0974-1208.192070-14","first-page":"170","volume-title":"Contamination by seminal plasma factors during sperm selection","author":"Bj\u00f6rndahl","year":"2005","journal-title":"J Androl"},{"key":"key-10.4103\/0974-1208.192070-15","first-page":"219","volume-title":"Are tests of sperm DNA damage clinically useful.Pros and cons?","author":"Zini","year":"2009","journal-title":"J Androl"},{"key":"key-10.4103\/0974-1208.192070-16","first-page":"759","volume-title":"Comparative evaluation of two density gradient preparations for sperm separation for medically assisted conception","author":"Chen","year":"1999","journal-title":"Hum Reprod"},{"key":"key-10.4103\/0974-1208.192070-17","first-page":"287","volume-title":"On the separation ability of various ficoll gradient solutions in zonal centrifugation","author":"Lavrenko","year":"1987","journal-title":"Anal Biochem"},{"key":"key-10.4103\/0974-1208.192070-18","doi-asserted-by":"crossref","unstructured":"Platsoucas CD, Good RA, Gupta S. Separation of human lymphocyte subpopulations by density gradient electrophoresis. I. Different mobilities of T (T mu, T alpha) and B lymphocytes from human tonsils. Cell Immunol 1980;51:238-49.","DOI":"10.1016\/0008-8749(80)90256-7"},{"key":"key-10.4103\/0974-1208.192070-19","first-page":"49","volume-title":"Partition of the hydrophobic compounds between two liquid phases of similar hydrophobicity","author":"Johansson","year":"1989","journal-title":"Chromatography"}],"container-title":["Journal of Human Reproductive Sciences"],"original-title":[],"language":"en","deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T13:29:30Z","timestamp":1786714170000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/0974-1208.192070"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":19,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2016]]}},"alternative-id":["192070"],"URL":"https:\/\/doi.org\/10.4103\/0974-1208.192070","relation":{},"ISSN":["0974-1208"],"issn-type":[{"value":"0974-1208","type":"print"}],"subject":[],"published":{"date-parts":[[2016]]}}}