{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T21:26:13Z","timestamp":1781558773325,"version":"3.54.5"},"reference-count":18,"publisher":"Scientific Scholar","issue":"01","license":[{"start":{"date-parts":[[2020,4,19]],"date-time":"2020-04-19T00:00:00Z","timestamp":1587254400000},"content-version":"vor","delay-in-days":2300,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Lab Physicians"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>\n          Background: Isolation of Brucellai is the gold standard in the laboratory diagnosis of brucellosis. As Brucellai is intracellular and the number of circulating bacteria is usually low, removal\/dilution of antibacterial substances, concentration of bacteria and optimal culture conditions may enhance the rate of isolation.<\/jats:p><jats:p>\n          Aims and Objectives: The objective of the following study was to compare the lysis concentration (LC), clot culture and conventional Castaneda blood culture techniques for the isolation rate and recovery time in the diagnosis of human brucellosis.<\/jats:p><jats:p>\n          Materials and Methods: Blood cultures by LC, clot culture and conventional method were performed in 169 patients who had antibody titers \u2265160 international units by the serum agglutination test.<\/jats:p><jats:p>\n          Results: Overall blood culture positivity was found to be 24.8%, 43.1% and 34.9% by conventional, LC and clot culture techniques in that order. The mean recovery time by LC and clot culture techniques was significantly less than conventional method, resulting in an overall difference of nearly 6 and 4 days respectively.<\/jats:p><jats:p>\n          Conclusions: For the isolation of Brucellai from blood, LC and clot culture techniques are better than the conventional technique.<\/jats:p>","DOI":"10.4103\/0974-2727.129084","type":"journal-article","created":{"date-parts":[[2014,3,19]],"date-time":"2014-03-19T06:24:50Z","timestamp":1395210290000},"page":"014-017","source":"Crossref","is-referenced-by-count":22,"title":["Comparison of Three Blood Culture Techniques in the Diagnosis of Human Brucellosis"],"prefix":"10.25259","volume":"6","author":[{"given":"Smita","family":"Mangalgi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, BLDEU\u2019s Shri B. M. Patil Medical College, Bijapur, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annapurna","family":"Sajjan","sequence":"additional","affiliation":[{"name":"Department of Microbiology, BLDEU\u2019s Shri B. M. Patil Medical College, Bijapur, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10894","published-online":{"date-parts":[[2020,4,19]]},"reference":[{"key":"key-10.4103\/0974-2727.129084-1","first-page":"539","volume-title":"Brucellosis in India-A review","author":"Mantur","year":"2008","journal-title":"J Biosci"},{"key":"key-10.4103\/0974-2727.129084-2","unstructured":"Corbel MJ. Brucellosis in Humans and Animals. WHO Press, Geneva, Switzerland; 2006."},{"key":"key-10.4103\/0974-2727.129084-3","first-page":"53","volume-title":"Laboratory diagnosis of brucellosis in a rural endemic area in Northeastern Spain","author":"Serra","year":"2004","journal-title":"Int Microbiol"},{"key":"key-10.4103\/0974-2727.129084-4","first-page":"1031","volume-title":"Evaluation of seven tests for diagnosis of human brucellosis in an area where the disease is endemic","author":"G\u00f3mez","year":"2008","journal-title":"Clin Vaccine Immunol"},{"key":"key-10.4103\/0974-2727.129084-5","first-page":"42","volume-title":"Seroprevalence of human brucellosis in rural endemic areas in eastern Turkey","author":"Vancelik","year":"2008","journal-title":"Trop Doct"},{"key":"key-10.4103\/0974-2727.129084-6","first-page":"104239","volume-title":"Seroprevalence study of human brucellosis by conventional tests and indigenous indirect enzyme-linked immunosorbent assay","author":"Agasthya","year":"2012","journal-title":"ScientificWorldJournal"},{"key":"key-10.4103\/0974-2727.129084-7","first-page":"612","volume-title":"Comparison of a dipstick assay for detection of Brucella-specific immunoglobulin M antibodies with other tests for serodiagnosis of human brucellosis","author":"Clavijo","year":"2003","journal-title":"Clin Diagn Lab Immunol"},{"key":"key-10.4103\/0974-2727.129084-8","first-page":"65","volume-title":"Serological diagnosis of brucellosis","author":"Nielsen","year":"2010","journal-title":"Sec Biol Med Sci"},{"key":"key-10.4103\/0974-2727.129084-9","first-page":"2325","volume-title":"Brucellosis","author":"Pappas","year":"2005","journal-title":"N Engl J Med"},{"key":"key-10.4103\/0974-2727.129084-10","first-page":"2417","volume-title":"Diagnosis of brucellosis by using blood cultures","author":"Ruiz","year":"1997","journal-title":"J Clin Microbiol"},{"key":"key-10.4103\/0974-2727.129084-11","first-page":"3437","volume-title":"Detection of Brucellae in blood cultures","author":"Yagupsky","year":"1999","journal-title":"J Clin Microbiol"},{"key":"key-10.4103\/0974-2727.129084-12","first-page":"586","volume-title":"Isolation of Brucella spp.from clinical specimens","author":"Etemadi","year":"1984","journal-title":"J Clin Microbiol"},{"key":"key-10.4103\/0974-2727.129084-13","first-page":"4327","volume-title":"Evaluation of conventional castaneda and lysis centrifugation blood culture techniques for diagnosis of human brucellosis","author":"Mantur","year":"2004","journal-title":"J Clin Microbiol"},{"key":"key-10.4103\/0974-2727.129084-14","first-page":"625","volume-title":"Comparison of culture techniques at different stages of brucellosis","author":"Espinosa","year":"2009","journal-title":"Am J Trop Med Hyg"},{"key":"key-10.4103\/0974-2727.129084-15","first-page":"388","volume-title":"Evaluation of blood clot cultures for isolation of Salmonella typhi, Salmonella paratyphi-A, and Brucella melitensis","author":"Escamilla","year":"1986","journal-title":"J Clin Microbiol"},{"key":"key-10.4103\/0974-2727.129084-16","first-page":"467","volume-title":"The study of salmonellosis with reference to salmonella typhi in enteric fever patients.Journal of Clinical and Diagnostic Research","author":"Abhijit","year":"2011","journal-title":""},{"key":"key-10.4103\/0974-2727.129084-17","first-page":"145","volume-title":"Comparison of whole blood culture and blood clot culture for the diagnosis of enteric fever","author":"Peerapur","year":"2013","journal-title":"JKIMSU"},{"key":"key-10.4103\/0974-2727.129084-18","first-page":"57","volume-title":"Diagnostic yield of blood clot culture in the accurate diagnosis of enteric fever and human brucellosis","author":"Mantur","year":"2007","journal-title":"Clin Lab"}],"container-title":["Journal of Laboratory Physicians"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.4103\/0974-2727.129084.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,24]],"date-time":"2023-12-24T08:13:40Z","timestamp":1703405620000},"score":1,"resource":{"primary":{"URL":"https:\/\/jlabphy.org\/comparison-of-three-blood-culture-techniques-in-the-diagnosis-of-human-brucellosis\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,1]]},"references-count":18,"journal-issue":{"issue":"01","published-online":{"date-parts":[[2020,4,19]]},"published-print":{"date-parts":[[2014,1]]}},"URL":"https:\/\/doi.org\/10.4103\/0974-2727.129084","relation":{},"ISSN":["0974-2727","0974-7826"],"issn-type":[{"value":"0974-2727","type":"print"},{"value":"0974-7826","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1]]}}}