{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T07:09:08Z","timestamp":1763968148207},"reference-count":22,"publisher":"Scientific Scholar","issue":"01","license":[{"start":{"date-parts":[[2020,4,19]],"date-time":"2020-04-19T00:00:00Z","timestamp":1587254400000},"content-version":"vor","delay-in-days":1935,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Lab Physicians"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>\n          Background: Serological diagnosis, based on antigenic fractions of the parasite can be used for the early diagnosis of human fascioliasis. The current study aimed to evaluate the efficacy of a 27 kDa immunodominant antigen of Fasciola hepatica adult worms, in an indirect enzyme-linked immunosorbent assay (ELISA) system for serological diagnosis of human fascioliasis.<\/jats:p><jats:p>\n          Materials and Methods: The immunodiagnosis of human fascioliasis, using a 27 kDa immunodominant antigen, purified from F. hepatica somatic antigens (SAs), was evaluated by Western blotting and ELISA with sera samples of human fascioliasis patients, healthy controls and patients with other parasitic infections.<\/jats:p><jats:p>\n          Results: Using western blotting, from 12 sera of fascioliasis patients, 11 sera (91.6%) detected the 27 kDa subunit. None of 30 samples from healthy controls or 32 sera from nonfascioliasis patients reacted with the 27 kDa antigen. Accordingly, sensitivity and specificity of the system was found to be 91.6% and 100%, respectively. The 27 kDa antigen was purified from the SAs and was used in an indirect ELISA system. Of 15 sera of fascioliasis patients, all (100%) were found to be positive by ELISA whereas only 4 cases (6.25%) of nonfascioliasis patients or healthy controls were false-positive by this system. Accordingly, the sensitivity and specificity of the test were 100% and 93.6%, respectively.<\/jats:p><jats:p>\n          Conclusion: Findings of this study demonstrated that both Western blotting and the indirect ELISA, based on the 27 kDa subunit of F. hepatica SA, are reliable methods for serodiagnosis of human fascioliasis.<\/jats:p>","DOI":"10.4103\/0974-2727.154781","type":"journal-article","created":{"date-parts":[[2015,4,8]],"date-time":"2015-04-08T09:16:58Z","timestamp":1428484618000},"page":"017-020","source":"Crossref","is-referenced-by-count":5,"title":["Performance of a 27 kDa Fasciola hepatica Antigen in the Diagnosis of Human Fascioliasis"],"prefix":"10.25259","volume":"7","author":[{"given":"Reza","family":"Shafiei","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bahador","family":"Sarkari","sequence":"additional","affiliation":[{"name":"Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyed Mahmoud","family":"Sadjjadi","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10894","published-online":{"date-parts":[[2020,4,19]]},"reference":[{"key":"key-10.4103\/0974-2727.154781-1","first-page":"41","volume-title":"Chapter 2.Fasciola, lymnaeids and human fascioliasis, with a global overview on disease transmission, epidemiology, evolutionary genetics, molecular epidemiology and control","author":"Mas-Coma","year":"2009","journal-title":"Adv Parasitol"},{"key":"key-10.4103\/0974-2727.154781-2","first-page":"400","volume-title":"Fascioliasis: US, CT, and MRI findings with new observations","author":"Kabaalioglu","year":"2000","journal-title":"Abdom Imaging"},{"key":"key-10.4103\/0974-2727.154781-3","unstructured":"Hillyer GV. Immunodiagnosis of human and animal fascioliasis. In: Dalton JP, editor. Fasciolosis. Oxon, Wallingford, London, UK: CABI Publishing; 1998. p. 435-48."},{"key":"key-10.4103\/0974-2727.154781-4","first-page":"417","volume-title":"Immunodiagnosis of Fasciola hepatica infection (fascioliasis) in a human population in the Bolivian Altiplano using purified cathepsin L cysteine proteinase","author":"O\u2032Neill","year":"1998","journal-title":"Am J Trop Med Hyg"},{"key":"key-10.4103\/0974-2727.154781-5","first-page":"977","volume-title":"Evaluation of Fas2-ELISA for the serological detection of Fasciola hepatica infection in humans","author":"Espinoza","year":"2007","journal-title":"Am J Trop Med Hyg"},{"key":"key-10.4103\/0974-2727.154781-6","first-page":"749","volume-title":"Short report: Immunodiagnosis of human fascioliasis using recombinant Fasciola hepatica cathepsin L1 cysteine proteinase","author":"O\u2032Neill","year":"1999","journal-title":"Am J Trop Med Hyg"},{"key":"key-10.4103\/0974-2727.154781-7","first-page":"69","volume-title":"Comparison of adult somatic and cysteine proteinase antigens of Fasciola gigantica in enzyme linked immunosorbent assay for serodiagnosis of human fasciolosis","author":"Rokni","year":"2003","journal-title":"Acta Trop"},{"key":"key-10.4103\/0974-2727.154781-8","first-page":"175","volume-title":"Diagnosis of human fasciolosis in the Gilan province of Northern Iran: Application of cathepsin L-ELISA","author":"Rokni","year":"2002","journal-title":"Diagn Microbiol Infect Dis"},{"key":"key-10.4103\/0974-2727.154781-9","first-page":"18","volume-title":"Evaluation of fast-ELISA versus standard-ELISA to diagnose human fasciolosis","author":"Rahimi","year":"2011","journal-title":"Arch Iran Med"},{"key":"key-10.4103\/0974-2727.154781-10","first-page":"1266","volume-title":"A consistent PCR-RFLP assay based on ITS-2 ribosomal DNA for differentiation of Fasciola species","author":"Shafiei","year":"2013","journal-title":"Iran J Basic Med Sci"},{"key":"key-10.4103\/0974-2727.154781-11","first-page":"7","volume-title":"Application of western blotting using native antigen B for serodiagnosis of human cystic echinococcosis","author":"Sarkari","year":"2007","journal-title":"Iran J Parasitol"},{"key":"key-10.4103\/0974-2727.154781-12","unstructured":"Tech Tip N.51, T.S. Available from: http:\/\/www.thermo.com\/pierce. [Last accessed on 2011 Sep 10]."},{"key":"key-10.4103\/0974-2727.154781-13","first-page":"15","volume-title":"Seroprevalence of human fasciolosis in a new-emerging focus of fasciolosis in Yasuj district, southwest of Iran","author":"Sarkari","year":"2012","journal-title":"Iran J Parasitol"},{"key":"key-10.4103\/0974-2727.154781-14","first-page":"713","volume-title":"Immunodiagnosis of human fascioliasis using an antigen of Fasciola gigantica adult worm with the molecular mass of 27 kDa by a dot-ELISA","author":"Intapan","year":"2003","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"key-10.4103\/0974-2727.154781-15","first-page":"660","volume-title":"Fasciola hepatica cysteine proteinases: Immunodominant antigens in human fascioliasis","author":"Cordova","year":"1997","journal-title":"Am J Trop Med Hyg"},{"key":"key-10.4103\/0974-2727.154781-16","first-page":"121","volume-title":"Usefulness of 8 kDa protein of Fasciola hepatica in diagnosis of fascioliasis","author":"Kim","year":"2003","journal-title":"Korean J Parasitol"},{"key":"key-10.4103\/0974-2727.154781-17","first-page":"355","volume-title":"Fasciola hepatica saposin-like protein-2-based ELISA for the serodiagnosis of chronic human fascioliasis","author":"Figueroa-Santiago","year":"2011","journal-title":"Diagn Microbiol Infect Dis"},{"key":"key-10.4103\/0974-2727.154781-18","first-page":"176","volume-title":"Diagnostic efficacy of monoclonal antibody based sandwich enzyme linked immunosorbent assay (ELISA) for detection of Fasciola gigantica excretory\/secretory antigens in both serum and stool","author":"Demerdash","year":"2011","journal-title":"Parasit Vectors"},{"key":"key-10.4103\/0974-2727.154781-19","first-page":"18","volume-title":"Partially purified fraction (PPF) antigen from adult Fasciola gigantica for the serodiagnosis of human fascioliasis using Dot-ELISA technique","author":"Dalimi","year":"2004","journal-title":"Ann Saudi Med","ISSN":"http:\/\/id.crossref.org\/issn\/0256-4947","issn-type":"print"},{"key":"key-10.4103\/0974-2727.154781-20","first-page":"374","volume-title":"A cysteine proteinase from the liver fluke Fasciola spp.: Purification, characterization, localization and application to immunodiagnosis","author":"Yamasaki","year":"1989","journal-title":"Jpn J Parasitol"},{"key":"key-10.4103\/0974-2727.154781-21","first-page":"463","volume-title":"Evaluation of specific Fasciola antigen in the immunodiagnosis of human fascioliasis in Egypt","author":"Shaker","year":"1994","journal-title":"J Egypt Soc Parasitol"},{"key":"key-10.4103\/0974-2727.154781-22","first-page":"213","volume-title":"Antigenic components of somatic extract from adult Fasciola gigantica recognized by infected human sera","author":"Maleewong","year":"1997","journal-title":"Asian Pac J Allergy Immunol"}],"container-title":["Journal of Laboratory Physicians"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.4103\/0974-2727.154781.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,24]],"date-time":"2023-12-24T08:13:54Z","timestamp":1703405634000},"score":1,"resource":{"primary":{"URL":"https:\/\/jlabphy.org\/performance-of-a-27-kda-fasciola-hepatica-antigen-in-the-diagnosis-of-human-fascioliasis\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,1]]},"references-count":22,"journal-issue":{"issue":"01","published-online":{"date-parts":[[2020,4,19]]},"published-print":{"date-parts":[[2015,1]]}},"URL":"https:\/\/doi.org\/10.4103\/0974-2727.154781","relation":{},"ISSN":["0974-2727","0974-7826"],"issn-type":[{"value":"0974-2727","type":"print"},{"value":"0974-7826","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,1]]}}}