{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:04:55Z","timestamp":1791216295847,"version":"4.1.0"},"reference-count":10,"publisher":"Scientific Scholar","issue":"02","license":[{"start":{"date-parts":[[2020,1,29]],"date-time":"2020-01-29T00:00:00Z","timestamp":1580256000000},"content-version":"vor","delay-in-days":3499,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Lab Physicians"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>\n          Background \/ Aims: Septicemia is one of the important causes of mortality and morbidity in neonates and children. Blood culture is the gold standard for the diagnosis. Emergence of multidrug resistant bacterial strains is a major problem in the management of sepsis. Present study was undertaken to identify the common bacterial pathogens associated with pediatric sepsis and to determine their antibiotic susceptibility pattern.<\/jats:p><jats:p>\n          Materials and Methods: Blood cultures from 185 suspected cases of sepsis were examined. The growths from the subcultures were identified by conventional biochemical tests. Antibiotic susceptibility testing was performed by modified Kirby-Bauer disk diffusion method and drug resistant strains in primary screening were further processed for extended spectrum beta lactamases (ESBL) and methicillin resistant Staphylococcus aureus (MRSA) status by combination disk method (ESBL) and oxacillin disk diffusion method (MRSA).<\/jats:p><jats:p>\n          Results: Out of the 185 cultures obtained from suspected cases, 81 (44%) were culture positive. Fifty-two (35%) of the culture isolates were Gram negative bacilli. Twenty-eight (64%) of the isolates were Gram positive cocci. One case was of mixed infection. The prevalence of MRSA in 41 strains of S. aureus was found to be 29% (12 strains). The overall prevalence of ESBL producers among 28 Gram negative bacterial isolates was found to be 32% (9 strains).<\/jats:p><jats:p>\n          Conclusion: This study stresses the need for the continuous screening and surveillance for antibiotic resistance in pediatric care unit.<\/jats:p>","DOI":"10.4103\/0974-2727.72156","type":"journal-article","created":{"date-parts":[[2010,10,29]],"date-time":"2010-10-29T06:08:35Z","timestamp":1288332515000},"page":"085-088","source":"Crossref","is-referenced-by-count":25,"title":["Bacteriologic Profile and Antibiogram of Blood Culture Isolates in a Pediatric Care Unit"],"prefix":"10.25259","volume":"2","author":[{"given":"Kavitha","family":"Prabhu","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Yenepoya Medical College, Deralakatte, Mangalore, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sevitha","family":"Bhat","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Yenepoya Medical College, Deralakatte, Mangalore, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunil","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Yenepoya Medical College, Deralakatte, Mangalore, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10894","published-online":{"date-parts":[[2020,1,29]]},"reference":[{"key":"key-10.4103\/0974-2727.72156-1","first-page":"110","volume-title":"Bacterial isolates from blood cultures of children with suspected septicaemia in Calabar, Nigeria","author":"Meremkwer","year":"2005","journal-title":"BMC Infect Dis"},{"key":"key-10.4103\/0974-2727.72156-2","first-page":"72","volume-title":"Bacteremia in hospitalized children-A one year prospective study","author":"De","year":"1995","journal-title":"Indian J Med Microbiol","ISSN":"https:\/\/id.crossref.org\/issn\/0255-0857","issn-type":"print"},{"key":"key-10.4103\/0974-2727.72156-3","unstructured":"Enrione MA, Powell KR. Sepsis, septic shock and systemic inflammatory response syndrome. In: Nelson textbook of paediatrics. Kleigman RM, Behraman RE, Jenson HB, Stanton BF, editors. 18 th ed. Philadelphia: WB Saunders; 2007. p. 1094."},{"key":"key-10.4103\/0974-2727.72156-4","unstructured":"Richard B, Thomson JR. Specimen collection, transport and processing: Bacteriology. In: Manual of clinical Microbiology. Murray PR, Baron EJ, Jorgensen JH, Landry ML, Pfaller MA, editors. 11 th ed. Washington DC: ASM Press; 2007. p. 309."},{"key":"key-10.4103\/0974-2727.72156-5","first-page":"222","volume-title":"A review of bacterial isolates in blood cultures of children with septicaemia in a Nigerian tertiary Hospital","author":"Nwadioha","year":"2010","journal-title":"Afr J Microbiol Res"},{"key":"key-10.4103\/0974-2727.72156-6","unstructured":"Forbes BA, Sahm DF, Weissfeld AS. In: Bailey and Scott\u2032s Diagnostic Microbiology. 12 th ed. Missouri: Mosby Elsevier; 2007. p. 779."},{"key":"key-10.4103\/0974-2727.72156-7","unstructured":"NCCLS performance standards for antimicrobial susceptibility testing. Twelfth international supplement. NCCLS document M100-S12 [ISBN 1-56238-454-6]. NCCLS, 940 WEST VALLEY Road, suite 1400, Wayne Pennsylvania 19087-1898 USA, 2002."},{"key":"key-10.4103\/0974-2727.72156-8","first-page":"1000","volume-title":"Guidelines for the laboratory diagnosis of and susceptibility testing of methicillin resistant Staphylococcus aureus (MRSA)","author":"Brown","year":"2005","journal-title":"J AntiMicrob Chemothe"},{"key":"key-10.4103\/0974-2727.72156-9","first-page":"1877","volume-title":"Detection of extended spectrum beta lactamases in members of family enterobacteriaceae-comparison of double disk and 3 D test","author":"Thomson","year":"1992","journal-title":"Antimicrob Agents Chemother"},{"key":"key-10.4103\/0974-2727.72156-10","first-page":"429","volume-title":"Invasive bacteria isolates from children with severe infections in a Nigerian hospital","author":"Onipede","year":"2009","journal-title":"J Infect Dev Ctries"}],"container-title":["Journal of Laboratory Physicians"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.4103\/0974-2727.72156.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,24]],"date-time":"2023-12-24T08:18:36Z","timestamp":1703405916000},"score":1,"resource":{"primary":{"URL":"https:\/\/jlabphy.org\/bacteriologic-profile-and-antibiogram-of-blood-culture-isolates-in-a-pediatric-care-unit\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,7]]},"references-count":10,"journal-issue":{"issue":"02","published-online":{"date-parts":[[2020,1,29]]},"published-print":{"date-parts":[[2010,7]]}},"URL":"https:\/\/doi.org\/10.4103\/0974-2727.72156","relation":{},"ISSN":["0974-2727","0974-7826"],"issn-type":[{"value":"0974-2727","type":"print"},{"value":"0974-7826","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,7]]}}}