{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T10:00:33Z","timestamp":1787047233653,"version":"build-2736575974"},"reference-count":11,"publisher":"ScienceOpen","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>The <jats:italic>in vitro<\/jats:italic> dose calibration curves using conventional biological dosimetry \u2013 dicentric chromosome assay (DCA) and premature chromosome condensation (PCC) assay \u2013 were performed for the first time in Thailand for reconstruction of radiation dose in the exposed individuals. The peripheral blood lymphocyte samples from healthy donors were irradiated with <jats:sup>137<\/jats:sup>Cs source at a dose rate of 0.652 Gy\/min to doses of 0.1, 0.25, 0.5, 0.75, 1, 2, 3, 4, and 5 Gy for DCA technique, and 5, 10, 15, 20, and 25 Gy for PCC technique. The blood samples were cultured and processed following the standard procedure as prescribed in the International Atomic Energy Agency report with slight modifications. The yield of dicentrics with dose from at least 1000 metaphases or 100 dicentrics was fitted to a linear quadratic model using Chromosome Aberration Calculation Software (CABAS, version 2.0) whereas those of PCC rings with dose from 100 rings was fitted to a linear quadratic equation at doses from 0 to 15 Gy. These curves will be useful for <jats:italic>in vitro<\/jats:italic> dose reconstruction and can support the preparedness for overexposure to radiation among public or occupational workers and eventual radiological accident in Thailand.<\/jats:p>","DOI":"10.4103\/2041-9414.197165","type":"journal-article","created":{"date-parts":[[2016,12,30]],"date-time":"2016-12-30T06:31:04Z","timestamp":1483079464000},"source":"Crossref","is-referenced-by-count":7,"title":["Establishment of Dose-response Curves for Dicentrics and Premature Chromosome Condensation for Radiological Emergency Preparedness in Thailand"],"prefix":"10.14293","volume":"7","author":[{"given":"Benchawan","family":"Rungsimaphorn","sequence":"first","affiliation":[{"name":"Department of Applied Radiation and Isotopes, Faculty of Science, Kasetsart University, Bangkok, Thailand"},{"name":"Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Budsaba","family":"Rerkamnuaychoke","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanwisa","family":"Sudprasert","sequence":"additional","affiliation":[{"name":"Department of Applied Radiation and Isotopes, Faculty of Science, Kasetsart University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5403","published-online":{"date-parts":[[2016]]},"reference":[{"key":"ref1","volume-title":"IAEA. The Radiological Accident in Samut Prakan","year":"2002"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1667\/RR1843.1","article-title":"Premature chromosome condensation (PCC) assay for dose assessment in mass casualty accidents","volume":"173","author":"C Lindholm","year":"2010","journal-title":"Radiat Res"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1080\/095530098141582","article-title":"Detection of total-and partial-body irradiation in a monkey model: A comparative study of chromosomal aberration, micronucleus and premature chromosome condensation assays","volume":"74","author":"F Darroudi","year":"1998","journal-title":"Int J Radiat Biol"},{"key":"ref4","volume-title":"IAEA. Cytogenetic dosimetry: Applications in preparedness for and response to radiation emergencies: EPR-biodosimetry","year":"2011"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1269\/jrr.33.SUPPLEMENT_231","article-title":"Robot system for preparing lymphocyte chromosome","volume":"33","author":"I Hayata","year":"1992","journal-title":"J Radiat Res"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1002\/cyto.a.21154","article-title":"Optimization of calyculin A-induced premature chromosome condensation assay for chromosome aberration studies","volume":"79","author":"T Miura","year":"2011","journal-title":"Cytometry A"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1093\/rpd\/ncu124","article-title":"Cytogenetic monitoring of nuclear workers occupationally exposed to ionising radiation","volume":"159","author":"B Griciene","year":"2014","journal-title":"Radiat Prot Dosimetry"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1093\/rpd\/nct285","article-title":"Biological dose assessment after low-dose overexposures in nuclear medicine","volume":"162","author":"M Pinto","year":"2014","journal-title":"Radiat Prot Dosimetry"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.mrgentox.2010.03.008","article-title":"Biodosimetry for high dose accidental exposures by drug induced premature chromosome condensation (PCC) assay","volume":"699","author":"S Balakrishnan","year":"2010","journal-title":"Mutat Res"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.biochi.2012.10.001","article-title":"Chromatin condensation dynamics and implications of induced premature chromosome condensation","volume":"95","author":"M Ravi","year":"2013","journal-title":"Biochimie"},{"key":"ref11","volume-title":"International Organization for Standardization. ISO 19238. Radiation Protection-Performance Criteria for Service Laboratories Performing Biological Dosimetry by Cytogenetics","year":"2014"}],"container-title":["Genome Integrity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scienceopen.com\/document_file\/c69406b7-56b2-434d-9f28-9ed7b8437729\/ScienceOpen\/c69406b7-56b2-434d-9f28-9ed7b8437729.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scienceopen.com\/hosted-document?doi=10.4103\/2041-9414.197165","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T11:10:11Z","timestamp":1694776211000},"score":1,"resource":{"primary":{"URL":"https:\/\/scienceopen.com\/hosted-document?doi=10.4103\/2041-9414.197165"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":11,"URL":"https:\/\/doi.org\/10.4103\/2041-9414.197165","relation":{},"ISSN":["2041-9414"],"issn-type":[{"value":"2041-9414","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2016]]},"published":{"date-parts":[[2016]]}}}