{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T12:35:11Z","timestamp":1711715711813},"reference-count":14,"publisher":"ScienceOpen","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>The aim of this study was not only to obtain basic technical prerequisites for the establishment of capacity of biological dosimetry at the Ghana Atomic Energy Commission (GAEC) but also to stimulate interest in biological dosimetry research in Ghana and Sub-Saharan Africa. Peripheral blood from four healthy donors was exposed to different doses (0\u20136 Gy) of gamma rays from a radiotherapy machine and lymphocytes were subsequently stimulated, cultured, and processed according to standard protocols for 48\u201350 h. Processed cells were analyzed for the frequencies of dicentric and centric ring chromosomes. Radiation dose delivered to the experimental model was verified using GafChromic\u00ae EBT films in parallel experiments. Basic technical prerequisites for the establishment of capacity of biological dosimetry in the GAEC have been realized and expertise in the dicentric chromosome assay consolidated. We successfully obtained preliminary cytogenetic data for a dose-response relationship of the irradiated blood lymphocytes. The data strongly indicate the existence of significant linear (\u03b1) and quadratic (\u03b2) components and are consistent with those published for the production of chromosome aberrations in comparable absorbed dose ranges.<\/jats:p>","DOI":"10.4103\/2041-9414.197173","type":"journal-article","created":{"date-parts":[[2016,12,30]],"date-time":"2016-12-30T06:31:04Z","timestamp":1483079464000},"source":"Crossref","is-referenced-by-count":1,"title":["Towards Establishing Capacity for Biological Dosimetry at Ghana Atomic Energy Commission"],"prefix":"10.14293","volume":"7","author":[{"given":"Daniel Gyingiri","family":"Achel","sequence":"first","affiliation":[{"name":"Applied Radiation Biology Centre, Radiological and Medical Sciences Research Institute, Ghana Atomic Energy Commission, Legon, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elom","family":"Achoribo","sequence":"additional","affiliation":[{"name":"Applied Radiation Biology Centre, Radiological and Medical Sciences Research Institute, Ghana Atomic Energy Commission, Legon, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Agbenyegah","sequence":"additional","affiliation":[{"name":"Applied Radiation Biology Centre, Radiological and Medical Sciences Research Institute, Ghana Atomic Energy Commission, Legon, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rudolph M.","family":"Adaboro","sequence":"additional","affiliation":[{"name":"Applied Radiation Biology Centre, Radiological and Medical Sciences Research Institute, Ghana Atomic Energy Commission, Legon, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shadrack","family":"Donkor","sequence":"additional","affiliation":[{"name":"Applied Radiation Biology Centre, Radiological and Medical Sciences Research Institute, Ghana Atomic Energy Commission, Legon, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nana A. K.","family":"Adu-Bobi","sequence":"additional","affiliation":[{"name":"Applied Radiation Biology Centre, Radiological and Medical Sciences Research Institute, Ghana Atomic Energy Commission, Legon, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akwasi A.","family":"Agyekum","sequence":"additional","affiliation":[{"name":"Applied Radiation Biology Centre, Radiological and Medical Sciences Research Institute, Ghana Atomic Energy Commission, Legon, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felicia","family":"Akuamoa","sequence":"additional","affiliation":[{"name":"Applied Radiation Biology Centre, Radiological and Medical Sciences Research Institute, Ghana Atomic Energy Commission, Legon, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel N.","family":"Tagoe","sequence":"additional","affiliation":[{"name":"National Centre for Radiotherapy and Nuclear Medicine, Korle-Bu Teaching Hospital, Accra, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kofi A.","family":"Kyei","sequence":"additional","affiliation":[{"name":"National Centre for Radiotherapy and Nuclear Medicine, Korle-Bu Teaching Hospital, Accra, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joel","family":"Yarney","sequence":"additional","affiliation":[{"name":"National Centre for Radiotherapy and Nuclear Medicine, Korle-Bu Teaching Hospital, Accra, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Serafin","sequence":"additional","affiliation":[{"name":"Department of Medical Imaging and Clinical Oncology, Division of Radiobiology, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, Cape Town, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John M.","family":"Akudugu","sequence":"additional","affiliation":[{"name":"Department of Medical Imaging and Clinical Oncology, Division of Radiobiology, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, Cape Town, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"5403","published-online":{"date-parts":[[2016]]},"reference":[{"key":"ref1","first-page":"27","article-title":"Emergency dosimetry using ceramic components in personal electronic devices","author":"EC Kouroukla","year":"2014","journal-title":"Int J Mod Phys Conf Ser"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1097\/00004032-200305000-00001","article-title":"Dose response relationships for acute ionizing-radiation lethality","volume":"84","author":"GH Anno","year":"2003","journal-title":"Health Phys"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1073\/pnas.48.4.522","article-title":"Types and rates of x-ray-induced chromosome aberrations in human blood irradiated in vitro","volume":"48","author":"MA Bender","year":"1962","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref4","volume-title":"IAEA. 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Health risk assessment from the nuclear accident after the 2011 Great East Japan earthquake and tsunami based on a preliminary dose estimation","year":"2011"},{"key":"ref7","first-page":"17","article-title":"Interpretation of chromosomal aberration analysis in biological dosimetry","volume":"25","author":"BS Rao","year":"2002","journal-title":"Radiat Prot Environ"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/S1383-5718(99)00191-6","article-title":"Chromosomal aberrations in peripheral lymphocytes from healthy subjects as detected in first cell division","volume":"446","author":"G Stephan","year":"1999","journal-title":"Mutat Res"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"2392","DOI":"10.1118\/1.1776691","article-title":"Dosimetric properties of improved GafChromic films for seven different digitizers","volume":"31","author":"S Devic","year":"2004","journal-title":"Med Phys"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"2245","DOI":"10.1118\/1.1929253","article-title":"Precise radiochromic film dosimetry using a flat-bed document scanner","volume":"32","author":"S Devic","year":"2005","journal-title":"Med Phys"},{"key":"ref11","volume-title":"International Specialty Products (ISP). Gafchromic \u00ae EBT: Self-developing film for radiotherapy dosimetry","year":"2007"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"6271","DOI":"10.1118\/1.3512792","article-title":"A comprehensive study of the Gafchromic EBT2 radiochromic film. A comparison with EBT","volume":"37","author":"C Andr\u00e9s","year":"2010","journal-title":"Med Phys"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1016\/S0969-8043(00)00054-3","article-title":"The role of cytogenetics in early triage of radiation casualties","volume":"52","author":"DC Lloyd","year":"2000","journal-title":"Appl Radiat Isot"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/S0033-7560(75)80001-9","article-title":"Radiation-induced chromosomal aberrations in the plant Tradescantia: Dose-response curves I. 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