{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:51:18Z","timestamp":1788871878599,"version":"build-2803163510"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,10,1]],"date-time":"2011-10-01T00:00:00Z","timestamp":1317427200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2011,10,1]],"date-time":"2011-10-01T00:00:00Z","timestamp":1317427200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["1K08HL105536\u201001A1 (BBG)"],"award-info":[{"award-number":["1K08HL105536\u201001A1 (BBG)"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Pulm. circ."],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:p>\n                    Schistosomiasis\u2010associated pulmonary arterial hypertension (PAH) is one of the most common causes of pulmonary hypertension worldwide. A potential contributing mechanism to the pathogenesis of this disease is a localized immune reaction to retained and persistent parasite\u2010derived antigens. We sought to identify Schistosoma\u2010derived egg antigens present in the lungs of individuals who died of the disease. We obtained 18 lung samples collected at autopsy from individuals who died of schistosomiasis\u2010associated PAH in Brazil. A rabbit polyclonal antibody was created to known\n                    <jats:italic>Schistosoma mansoni<\/jats:italic>\n                    \u2010soluble egg antigen (SEA). Histologic assessment and immunostaining of the human tissue was performed, along with immunostaining and immunoblotting of lung tissue from mice experimentally infected with\n                    <jats:italic>S. mansoni<\/jats:italic>\n                    . All 18 lung samples had evidence of pulmonary vascular remodeling with plexiform lesions and arterial medial thickening, but no visible eggs were seen. The anti\u2010SEA antibody detected\n                    <jats:italic>S. mansoni<\/jats:italic>\n                    egg antigens in visible eggs in mouse lung and human intestine specimens, but did not identify a significant amount of egg antigen in the human lung specimens. In mouse granulomas containing degraded eggs, we observed colocalization of egg antigens and macrophage lysosomes. In conclusion, there is unlikely to be a significant amount of persistent parasite\u2010derived antigens within the lungs of individuals who die of schistosomiasis\u2010associated PAH. This suggests that retained and persistent parasite proteins are not contributing to a localized immune response in the pathogenesis of this disease.\n                  <\/jats:p>","DOI":"10.4103\/2045-8932.93544","type":"journal-article","created":{"date-parts":[[2012,3,5]],"date-time":"2012-03-05T18:14:17Z","timestamp":1330971257000},"page":"456-461","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":34,"title":["Significant Intrapulmonary\n                    <i>Schistosoma<\/i>\n                    Egg Antigens are not Present in Schistosomiasis\u2010Associated Pulmonary Hypertension"],"prefix":"10.1002","volume":"1","author":[{"given":"Brian B.","family":"Graham","sequence":"first","affiliation":[{"name":"Department of Medicine Program in Translational Lung Research University of Colorado Denver Denver CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob","family":"Chabon","sequence":"additional","affiliation":[{"name":"Department of Medicine Program in Translational Lung Research University of Colorado Denver Denver CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Bandeira","sequence":"additional","affiliation":[{"name":"Department of Cardiology Memorial S. Jose Hospital Universidade de Pernambuco in Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciano","family":"Espinheira","sequence":"additional","affiliation":[{"name":"Department of Pathology Hospital Prof. Edgard Santos Universidade Federal da Bahia Salvador Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghazwan","family":"Butrous","sequence":"additional","affiliation":[{"name":"University of Kent Kent UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rubin M.","family":"Tuder","sequence":"additional","affiliation":[{"name":"Department of Medicine Program in Translational Lung Research University of Colorado Denver Denver CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro801"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.19.6.572"},{"key":"e_1_2_7_4_1","first-page":"2028","article-title":"Prevalence of pulmonary hypertension in patients with hepatosplenic Mansonic schistosomiasis\u2010prospective study","volume":"50","author":"Cleva R","year":"2003","journal-title":"Hepatogastroenterology"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.108.803221"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0029-1233307"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1982.31.779"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(67)90253-7"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-8703(53)90179-3"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1970.19.305"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(57)90043-3"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.100063"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.2307\/3281829"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.132.3.488"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.101.1.85"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02761987000800005"},{"key":"e_1_2_7_17_1","first-page":"167","article-title":"Cor Pulmonale in Manson's Schistosomiasis I. Frequency in Necropsy Material; Pulmonary Vascular Changes Caused by Schistosome Ova","volume":"30","author":"Faria JL","year":"1954","journal-title":"Am J Pathol"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(07)70053-1"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.03.00038903"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03285.x"},{"key":"e_1_2_7_21_1","first-page":"A4895","article-title":"Praziquantel prevents progression of right ventricular hypertrophy in a mouse model of Schistosomiasis","volume":"181","author":"Crosby A","year":"2010","journal-title":"Am J Respir Crit Care Med"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00389899"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(00)00125-X"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-010-0670-x"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.158.6.9805002"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.icb.7100014"}],"container-title":["Pulmonary Circulation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.4103\/2045-8932.93544","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.4103\/2045-8932.93544","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.4103\/2045-8932.93544","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,27]],"date-time":"2023-08-27T23:52:37Z","timestamp":1693180357000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.4103\/2045-8932.93544"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,10]]},"references-count":25,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2011,10]]}},"alternative-id":["10.4103\/2045-8932.93544"],"URL":"https:\/\/doi.org\/10.4103\/2045-8932.93544","archive":["Portico"],"relation":{},"ISSN":["2045-8940","2045-8940"],"issn-type":[{"value":"2045-8940","type":"print"},{"value":"2045-8940","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,10]]}}}