{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:38:23Z","timestamp":1790786303749,"version":"4.1.0"},"reference-count":44,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Molecular hydrogen (H\n                      <jats:sub>2<\/jats:sub>\n                      ) is an antioxidant and anti-inflammatory agent; however, the molecular mechanisms underlying its biological effects are largely unknown. Similar to other gaseous molecules such as inhalation anesthetics, H\n                      <jats:sub>2<\/jats:sub>\n                      is more soluble in lipids than in water. A recent study demonstrated that H\n                      <jats:sub>2<\/jats:sub>\n                      reduces radical polymerization-induced cellular damage by suppressing fatty acid peroxidation and membrane permeability. Thus, we sought to examine the effects of short exposure to H\n                      <jats:sub>2<\/jats:sub>\n                      on lipid composition and associated physiological changes in SH-SY5Y neuroblastoma cells. We analyzed cells by liquid chromatography-high-resolution mass spectrometry to define changes in lipid components. Lipid class analysis of cells exposed to H\n                      <jats:sub>2<\/jats:sub>\n                      for 1 hour revealed transient increases in glycerophospholipids including phosphatidylethanolamine, phosphatidylinositol, and cardiolipin. Metabolomic analysis also showed that H\n                      <jats:sub>2<\/jats:sub>\n                      exposure for 1 hour transiently suppressed overall energy metabolism accompanied by a decrease in glutathione. We further observed alterations to endosomal morphology by staining with specific antibodies. Endosomal transport of cholera toxin B to recycling endosomes localized around the Golgi body was delayed in H\n                      <jats:sub>2<\/jats:sub>\n                      -exposed cells. We speculate that H\n                      <jats:sub>2<\/jats:sub>\n                      -induced modification of lipid composition depresses energy production and endosomal transport concomitant with enhancement of oxidative stress, which transiently stimulates stress response pathways to protect cells.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/2045-9912.344973","type":"journal-article","created":{"date-parts":[[2022,7,18]],"date-time":"2022-07-18T07:36:33Z","timestamp":1658129793000},"page":"133-141","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":6,"title":["H2 -induced transient upregulation of phospholipids with suppression of energy 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