{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T02:39:45Z","timestamp":1784860785596,"version":"3.55.0"},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"01","license":[{"start":{"date-parts":[[2021,8,9]],"date-time":"2021-08-09T00:00:00Z","timestamp":1628467200000},"content-version":"vor","delay-in-days":1681,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Avicenna J Med"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Introduction: Extended-spectrum beta-lactamases (ESBLs) are the major cause of resistance to beta-lactam antibiotics such as penicillins, cephalosporins, and monobactams. They are derived from the narrow-spectrum beta-lactamases (TEM-1, TEM-2, or SHV-1) by mutations that alter the amino acid configuration around the enzyme active site. Aim: To determine the prevalence of ESBL (bla TEM , bla CTX-M , and bla SHV ) genes among the members of Enterobacteriaceae. Methodology: The present prospective study was carried out from January 2015 to June 2015 in the Department of Microbiology and Molecular Medicine of a Teaching Tertiary Care Hospital. A total of 526 urine samples were studied. Seventy-eight isolates were subjected to polymerase chain reaction for detection of ESBL genes. Results: In our study, ESBL genes were detected among 18 (45%) phenotypically confirmed ESBL producers and 20 (52.5%) phenotypically confirmed non-ESBL producers. The gene that predominated was bla TEM (48.7%), followed by bla CTX-M (7.6%) and bla SHV (5.1%). Conclusion: Definitive identification of ESBL genes is only possible by molecular detection methods. Phenotypic tests need to be evaluated periodically as their performance may change with the introduction of new enzymes.<\/jats:p>","DOI":"10.4103\/2231-0770.197508","type":"journal-article","created":{"date-parts":[[2017,1,4]],"date-time":"2017-01-04T06:30:13Z","timestamp":1483511413000},"page":"12-16","source":"Crossref","is-referenced-by-count":115,"title":["Prevalence of TEM, SHV, and CTX-M Beta-Lactamase genes in the urinary isolates of a tertiary care hospital"],"prefix":"10.1055","volume":"07","author":[{"given":"Trupti","family":"Bajpai","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sri Aurobindo Institute of Medical Sciences Medical College and PG Institute, Indore, Madhya Pradesh; Department of Biochemistry, IGNOU, SOS, New Delhi, India"},{"name":"Department of Biochemistry, IGNOU, SOS, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Pandey","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, IGNOU, SOS, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Varma","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Sri Aurobindo Institute of Medical Sciences Medical College and PG Institute, Indore, Madhya Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G S","family":"Bhatambare","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sri Aurobindo Institute of Medical Sciences Medical College and PG Institute, Indore, Madhya Pradesh; Department of Biochemistry, IGNOU, SOS, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2021,8,9]]},"container-title":["Avicenna Journal of Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.4103\/2231-0770.197508.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,8,9]],"date-time":"2021-08-09T23:53:24Z","timestamp":1628553204000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.4103\/2231-0770.197508"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1]]},"references-count":0,"journal-issue":{"issue":"01","published-online":{"date-parts":[[2021,8,9]]},"published-print":{"date-parts":[[2017,1]]}},"URL":"https:\/\/doi.org\/10.4103\/2231-0770.197508","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["2231-0770","2249-4464"],"issn-type":[{"value":"2231-0770","type":"print"},{"value":"2249-4464","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1]]}}}