{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T22:59:44Z","timestamp":1789340384378,"version":"build-2803163510"},"reference-count":37,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Ameloblastoma is a benign but aggressive epithelial odontogenic neoplasm of unknown etiology. The role of human papilloma virus (HPV) in the etiology of oral squamous cell carcinoma has prompted the investigation of HPV as an etiologic factor in ameloblastoma. This study aimed to determine the frequency of high-risk (HR) HPV in conventional ameloblastoma and the clinical parameters associated with infection.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      The study was approved by the ethical review boards of the institution. DNA was extracted from fresh tissue collected 750 \u03bcL of DNA\/RNA Shield (Zymo Research, United States) using Invitrogen PureLink Viral RNA\/DNA Mini Kit (Invitrogen, USA). The extracted DNA was assayed for the detection of 14 HR HPV types (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68) using Anyplex\u2122 II HPV HR Detection kit (Cat. No. HP7E00X) (Seegene Inc., Republic of Korea) on CFX-96TM Real-Time Polymerase Chain Reaction (PCR) System (Bio-Rad). Data on gender, age of patient, site of lesion, clinicohistological types of ameloblastoma and history of smoking, alcohol consumption, and practice of oral sex were collected. Data analysis was performed using analysis program SPSS version 25 and statistical significance was set at\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.05.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Two cases of conventional ameloblastoma were positive with HPV and none of the ameloblastic carcinoma cases were positive. The HPV 16 serotype was observed in both cases. While 5 of the cases had a history of alcohol consumption, none of these cases were positive for HPV serotype.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>HPV 16 positivity was detected in two cases of conventional ameloblastomas and none in ameloblastic carcinoma using real-time PCR. There was no effect of exposure to smoking, alcohol consumption, and practice of oral sex and HPV in the etiology of ameloblastoma. Data available are suggestive of a limited role of HPV in the etiology of ameloblastoma.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/aam.aam_102_23","type":"journal-article","created":{"date-parts":[[2024,4,24]],"date-time":"2024-04-24T12:03:54Z","timestamp":1713960234000},"page":"406-414","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":3,"title":["Low Detection of High-risk Human Papilloma Virus in Individuals with Ameloblastoma in a Tertiary Hospital in Lagos, Nigeria"],"prefix":"10.4103","volume":"23","author":[{"given":"Abdul-Warith Olaitan","family":"Akinshipo","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology\/Biology, Faculty of Dental Sciences, College of Medicine, University of Lagos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olumuyiwa Babalola","family":"Salu","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, Centre for Human and Zoonotic Virology, College of Medicine, University of Lagos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clement","family":"Oluwarotimi","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, Faculty of Dental Sciences, College of Medicine, University of Lagos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roosevelt Amaobichukwu","family":"Anyanwu","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, Centre for Human and Zoonotic Virology, College of Medicine, University of Lagos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernest Ebuka","family":"Aforka","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology\/Biology, Faculty of Dental Sciences, College of Medicine, University of Lagos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olajumoke Ajibola","family":"Effiom","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology\/Biology, Faculty of Dental Sciences, College of Medicine, University of Lagos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunday Aremu","family":"Omilabu","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, Centre for Human and Zoonotic Virology, College of Medicine, University of Lagos"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2024,4,24]]},"reference":[{"key":"R1-20260507","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1111\/odi.12646","article-title":"Ameloblastoma: Current etiopathological concepts and management","volume":"24","author":"Effiom","year":"2018","journal-title":"Oral Dis"},{"key":"R2-20260507","first-page":"168","article-title":"The World Health Organization classification of odontogenic lesions: A summary of the changes of the 2022 (5th) edition","volume":"38","author":"Soluk-Tekkesin","year":"2022","journal-title":"Turk Patoloji Derg"},{"key":"R3-20260507","doi-asserted-by":"crossref","first-page":"3326","DOI":"10.7150\/ijms.61500","article-title":"A retrospective study of 158 cases on the risk factors for recurrence in ameloblastoma","volume":"18","author":"Bi","year":"2021","journal-title":"Int J Med Sci"},{"key":"R4-20260507","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/s42047-019-0041-z","article-title":"Ameloblastomas: Current aspects of the new WHO classification in an analysis of 136 cases","volume":"2","author":"Cadavid","year":"2019","journal-title":"Surg Exp Pathol"},{"key":"R5-20260507","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1758-3284-2-1","article-title":"Unicystic ameloblastoma of the mandible \u2013 An unusual case report and review of literature","volume":"2","author":"Ramesh","year":"2010","journal-title":"Head Neck Oncol"},{"key":"R6-20260507","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1186\/s40902-017-0134-0","article-title":"Conservative management (marsupialization) of unicystic ameloblastoma: Literature review and a case report","volume":"39","author":"Kim","year":"2017","journal-title":"Maxillofac Plast Reconstr Surg"},{"key":"R7-20260507","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1111\/odi.13031","article-title":"Global incidence and profile of ameloblastoma: A systematic review and meta-analysis","volume":"26","author":"Hendra","year":"2020","journal-title":"Oral Dis"},{"key":"R8-20260507","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1080\/23320885.2021.1886854","article-title":"Ameloblastoma: Clinical presentation, multidisciplinary management and outcome","volume":"8","author":"Medina","year":"2021","journal-title":"Case Reports Plast Surg Hand Surg"},{"key":"R9-20260507","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1016\/j.jcms.2010.05.010","article-title":"Delayed soft tissue recurrence after treatment of ameloblastoma in a black African: Case report and review of the literature","volume":"39","author":"Adebayo","year":"2011","journal-title":"J Craniomaxillofac Surg"},{"key":"R10-20260507","first-page":"19","article-title":"Ameloblastoma: A review of recent molecular pathogenetic discoveries","volume":"7","author":"Brown","year":"2015","journal-title":"Biomark Cancer"},{"key":"R11-20260507","doi-asserted-by":"crossref","first-page":"djv403","DOI":"10.1093\/jnci\/djv403","article-title":"HPV involvement in head and neck cancers: Comprehensive assessment of biomarkers in 3680 patients","volume":"108","author":"Castellsagu\u00e9","year":"2016","journal-title":"J Natl Cancer Inst"},{"key":"R12-20260507","doi-asserted-by":"crossref","first-page":"e0203403","DOI":"10.1371\/journal.pone.0203403","article-title":"Differences in the viral genome between HPV-positive cervical and oropharyngeal cancer","volume":"13","author":"LeConte","year":"2018","journal-title":"PLoS One"},{"key":"R13-20260507","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.12669\/pjms.35.6.909","article-title":"Clinicopathological study of ameloblastoma and detection of human papilloma virus by immunohistochemistry","volume":"35","author":"Ali","year":"2019","journal-title":"Pak J Med Sci"},{"key":"R14-20260507","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1053\/joms.2003.50011","article-title":"A study of the relationship between ameloblastoma and human papilloma virus","volume":"61","author":"Namin","year":"2003","journal-title":"J Oral Maxillofac Surg"},{"key":"R15-20260507","doi-asserted-by":"crossref","first-page":"80","DOI":"10.3390\/v10020080","article-title":"The intersection of HPV epidemiology, genomics and mechanistic studies of HPV-mediated carcinogenesis","volume":"10","author":"Mirabello","year":"2018","journal-title":"Viruses"},{"key":"R16-20260507","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1038\/s41416-019-0602-7","article-title":"Human papillomavirus as a driver of head and neck cancers","volume":"122","author":"Sabatini","year":"2020","journal-title":"Br J Cancer"},{"key":"R17-20260507","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1093\/carcin\/bgx106","article-title":"Aetiological heterogeneity of head and neck squamous cell carcinomas: The role of human papillomavirus infections, smoking and alcohol","volume":"38","author":"Farsi","year":"2017","journal-title":"Carcinogenesis"},{"key":"R18-20260507","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1038\/modpathol.3800241","article-title":"Does HPV play a role in the etiopathogenesis of ameloblastoma? An immunohistochemical, in situ hybridization and polymerase chain reaction study of 18 cases using laser capture microdissection","volume":"18","author":"Migaldi","year":"2005","journal-title":"Mod Pathol"},{"key":"R19-20260507","doi-asserted-by":"crossref","first-page":"1653","DOI":"10.1002\/ijc.28811","article-title":"High-risk HPV types and head and neck cancer","volume":"135","author":"Michaud","year":"2014","journal-title":"Int J Cancer"},{"key":"R20-20260507","first-page":"1","article-title":"Prevalence of human papilloma virus in clinico-histologic types of ameloblastoma seen in Lagos university teaching hospital patients","volume":"1","author":"Salami","year":"2015","journal-title":"Afr J Oral Maxillofac Pathol Med"},{"key":"R21-20260507","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1016\/j.oooo.2018.06.014","article-title":"Biological profile of ameloblastoma and its location in the jaw in 1246 Nigerians","volume":"126","author":"Agbaje","year":"2018","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol"},{"key":"R22-20260507","doi-asserted-by":"crossref","first-page":"e20","DOI":"10.1016\/j.tripleo.2010.04.030","article-title":"Human papillomavirus in ameloblastoma","volume":"110","author":"Correnti","year":"2010","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol Endod"},{"key":"R23-20260507","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.jobcr.2017.04.002","article-title":"The anecdote of viral etiopathogenia in ameloblastoma and odontogenic keratocyst: Why don\u2019t we let it go?","volume":"7","author":"Khalele","year":"2017","journal-title":"J Oral Biol Craniofac Res"},{"key":"R24-20260507","doi-asserted-by":"crossref","first-page":"24","DOI":"10.13189\/cor.2013.010202","article-title":"Screening of ameloblastoma cases in Ibadan for HPV and EBV genes","volume":"1","author":"Onile","year":"2013","journal-title":"Cancer and Oncology Research"},{"key":"R25-20260507","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1053\/joms.2000.9573","article-title":"Presence of human papilloma viruses in intraosseous ameloblastoma","volume":"58","author":"Sand","year":"2000","journal-title":"J Oral Maxillofac Surg"},{"key":"R26-20260507","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1016\/0030-4220(89)90013-3","article-title":"Ameloblastoma in young persons: A clinicopathologic analysis and etiologic investigation","volume":"67","author":"Kahn","year":"1989","journal-title":"Oral Surg Oral Med Oral Pathol"},{"key":"R27-20260507","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3923\/ijv.2006.1.6","article-title":"Detection of human papillomavirus DNA in intraosseus ameloblastoma","volume":"2","author":"Mokhtari-Azad","year":"2006","journal-title":"Int J Virol"},{"key":"R28-20260507","doi-asserted-by":"crossref","first-page":"864820","DOI":"10.3389\/fonc.2022.864820","article-title":"Molecular detection methods in HPV-related cancers","volume":"12","author":"Williams","year":"2022","journal-title":"Front Oncol"},{"key":"R29-20260507","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1159\/000355952","article-title":"Human papillomavirus genomics: Past, present and future","volume":"45","author":"Harari","year":"2014","journal-title":"Curr Probl Dermatol"},{"key":"R30-20260507","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1177\/0300985813505879","article-title":"When tissue antigens and antibodies get along: Revisiting the technical aspects of immunohistochemistry \u2013 The red, brown, and blue technique","volume":"51","author":"Ramos-Vara","year":"2014","journal-title":"Vet Pathol"},{"key":"R31-20260507","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1007\/s10147-023-02327-9","article-title":"Association between human papillomavirus and oral cancer: A literature review","volume":"28","author":"Shigeishi","year":"2023","journal-title":"Int J Clin Oncol"},{"key":"R32-20260507","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1111\/j.1600-0714.1993.tb01039.x","article-title":"Detection of human papillomavirus DNA in an ameloblastoma using the in situ hybridization technique","volume":"22","author":"van Heerden","year":"1993","journal-title":"J Oral Pathol Med"},{"key":"R33-20260507","doi-asserted-by":"crossref","first-page":"2069","DOI":"10.1002\/ijc.34444","article-title":"A risk prediction model for head and neck cancers incorporating lifestyle factors, HPV serology and genetic markers","volume":"152","author":"Budhathoki","year":"2023","journal-title":"Int J Cancer"},{"key":"R34-20260507","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.canlet.2019.11.039","article-title":"Human papillomavirus vaccine against cervical cancer: Opportunity and challenge","volume":"471","author":"Wang","year":"2020","journal-title":"Cancer Lett"},{"key":"R35-20260507","doi-asserted-by":"crossref","first-page":"446","DOI":"10.5005\/jp-journals-10015-1771","article-title":"Low Frequency of Human Papillomavirus and Epstein-Barr Virus DNA in Ameloblastoma of Thai Patients","volume":"11","author":"Bumalee","year":"2020","journal-title":"World J Dent"},{"key":"R36-20260507","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.anndiagpath.2015.06.006","article-title":"Gnathic and peripheral ameloblastomas lack human papillomavirus DNA","volume":"19","author":"Verduin","year":"2015","journal-title":"Ann Diagn Pathol"},{"key":"R37-20260507","doi-asserted-by":"crossref","first-page":"20","DOI":"10.17352\/2455-1759.000016","article-title":"Ameloblastoma, human papillomavirus, and p16-is there an association","volume":"2","author":"Singh","year":"2016","journal-title":"Arch Otolaryngol Rhinol"}],"container-title":["Annals of African Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/aam.aam_102_23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T16:26:43Z","timestamp":1786724803000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/aam.aam_102_23"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,24]]},"references-count":37,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024]]}},"URL":"https:\/\/doi.org\/10.4103\/aam.aam_102_23","relation":{},"ISSN":["1596-3519","0975-5764"],"issn-type":[{"value":"1596-3519","type":"print"},{"value":"0975-5764","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,24]]},"assertion":[{"value":"2023-06-26","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-28","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}