{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:20:08Z","timestamp":1787761208011,"version":"build-2784847793"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"abstract":"Abstract<\/jats:title>\n \n Background:<\/jats:title>\n \n Helicobacter pylori<\/jats:italic>\n is an established pathogen in gastroduodenal diseases and has been increasingly implicated in hepatobiliary pathology. Several studies have identified\n H. pylori<\/jats:italic>\n DNA and antigens in gallbladder mucosa, bile, and gallstones, yet the exact nature of its association with gallstone disease remains unclear.\n <\/jats:p>\n <\/jats:sec>\n \n Objective:<\/jats:title>\n \n The objective of the study was to determine the association between\n H. pylori<\/jats:italic>\n infection in gallbladder tissue and bile with gallstone disease in patients undergoing elective cholecystectomy.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n This prospective observational study included 100 adult patients with cholelithiasis undergoing elective cholecystectomy. Intraoperative bile was tested using a rapid urease test (RUT). Excised gallbladders were assessed histologically and immunohistochemically for anti-\n H. pylori<\/jats:italic>\n antibodies. Clinical profiles, ultrasonography, and endoscopic findings were recorded. Ethical approval and informed consent were obtained.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n The mean age was 45.7 years; 71% were females. Typical biliary symptoms were present in 61%. RUT positivity was observed in 45% of bile samples, whereas immunohistochemistry detected\n H. pylori<\/jats:italic>\n in 28% of gallbladder specimens. A significant association was observed between RUT positivity and gallbladder tissue positivity (\n P<\/jats:italic>\n < 0.001). RUT showed 92.86% sensitivity and 73.61% specificity for gallbladder\n H. pylori<\/jats:italic>\n detection.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n \n A considerable proportion of patients with gallstone disease demonstrated\n H. pylori<\/jats:italic>\n positivity in bile or gallbladder tissue, supporting a possible association between\n H. pylori<\/jats:italic>\n infection and gallstone pathogenesis. Further mechanistic and longitudinal studies are warranted.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/aam.aam_123_26","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T04:00:09Z","timestamp":1780891209000},"update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Association of Helicobacter pylori Infection with Gallstone Disease: A Prospective Observational Study"],"prefix":"10.4103","author":[{"given":"Anuradha","family":"Dnyanmote","sequence":"first","affiliation":[{"name":"Department of General Surgery, Dr. D. Y. Patil Medical College, Hospital and Research Centre, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pravin","family":"Mane","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Dr. D. Y. Patil Medical College, Hospital and Research Centre, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taduri","family":"Alekhya","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Dr. D. Y. Patil Medical College, Hospital and Research Centre, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aparna","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Dr. D. Y. Patil Medical College, Hospital and Research Centre, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saumya","family":"Agarwal","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Dr. D. Y. Patil Medical College, Hospital and Research Centre, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2026,6,5]]},"container-title":["Annals of African Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/aam.aam_123_26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T15:27:14Z","timestamp":1787758034000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/aam.aam_123_26"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,5]]},"references-count":0,"URL":"https:\/\/doi.org\/10.4103\/aam.aam_123_26","relation":{},"ISSN":["1596-3519","0975-5764"],"issn-type":[{"value":"1596-3519","type":"print"},{"value":"0975-5764","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,5]]},"assertion":[{"value":"2026-02-06","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-22","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}