{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:22:02Z","timestamp":1787761322674,"version":"build-2784847793"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Dengue virus (DENV) infection remains a significant public health challenge in India, with increasing outbreaks and disease severity. The co-circulation of multiple DENV serotypes may influence clinical outcomes; however, data on serotype distribution and disease severity from many regions remain limited.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>A hospital-based observational study was conducted in Belagavi, Karnataka, during 2024. A total of 351 serum samples from clinically suspected dengue patients were tested for NS1 antigen, immunoglobulin G and immunoglobulin M by Enzyme linked Immunosorbent Assay, followed by confirmation and serotyping by Real-Time PCR. Demographic, clinical, and laboratory data were analyzed to assess associations between DENV serotypes, disease severity, and complications.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Of 351 samples tested, 102 were NS1-positive, and 80 were confirmed by RT-PCR. All four DENV serotypes were detected, with DENV-2 predominating (38.7%). Mixed-serotype infections were observed, most commonly DENV-1 with DENV-2 and DENV-1 with DENV-3. Most of the patients were young adults aged 21\u201340 years (56.3%) with male predominance (67.5%). Thrombocytopenia was common, though severe thrombocytopenia occurred in only 10%. No significant association was found between serotype and disease severity in primary or secondary infections. Pleural effusion was the most frequent complication, mainly associated with DENV-2.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The study demonstrates the co-circulation of all four DENV serotypes in Belagavi during 2024, with DENV-2 predominance. Disease severity was not significantly associated with specific serotypes, highlighting the role of host and disease-related factors. Continuous molecular surveillance is essential to monitor evolving serotype dynamics and inform dengue control strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/aam.aam_147_26","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T09:00:21Z","timestamp":1780909221000},"update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Dengue Virus Serotype Distribution and Disease Severity: Evidence from Molecular Surveillance in Karnataka, India"],"prefix":"10.4103","author":[{"given":"Madhumati","family":"Patil","sequence":"first","affiliation":[{"name":"Department of Microbiology, JNMC, KAHER University, Belagavi, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veena","family":"Shankar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, JNMC, KAHER University, Belagavi, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhishek","family":"Karishetty","sequence":"additional","affiliation":[{"name":"Department of Microbiology, JNMC, KAHER University, Belagavi, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nandadeep","family":"Jadhav","sequence":"additional","affiliation":[{"name":"Department of Microbiology, JNMC, KAHER University, Belagavi, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nagaraj","family":"Hallikeri","sequence":"additional","affiliation":[{"name":"Department of Microbiology, JNMC, KAHER University, Belagavi, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Biostatistics, JNMC, KAHER University, Belagavi, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2026,6,5]]},"container-title":["Annals of African Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/aam.aam_147_26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T15:32:24Z","timestamp":1787758344000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/aam.aam_147_26"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,5]]},"references-count":0,"URL":"https:\/\/doi.org\/10.4103\/aam.aam_147_26","relation":{},"ISSN":["1596-3519","0975-5764"],"issn-type":[{"value":"1596-3519","type":"print"},{"value":"0975-5764","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,5]]},"assertion":[{"value":"2026-02-12","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-27","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}