{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:47:19Z","timestamp":1787071639390,"version":"build-2736575974"},"reference-count":21,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T00:00:00Z","timestamp":1764115200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Context:<\/jats:title>\n                    <jats:p>ABO isoagglutinins, particularly anti-A and anti-B antibodies, are clinically significant in transfusion medicine due to their potential to cause hemolytic reactions in recipients of ABO-incompatible blood components. Evaluating the distribution of isoagglutinin titers in group O donors is essential for enhancing transfusion safety.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims:<\/jats:title>\n                    <jats:p>The aim of this study was to assess and compare anti-A and anti-B immunoglobulin (Ig) M and IgG titers among group O whole blood and plateletpheresis donors and explore their association with donor age and type.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>This cross-sectional study was conducted over a period of 1 year and included 300 voluntary blood donors at a tertiary care center. IgM titers were estimated using the saline cards, and IgG titers were measured on dithiothreitol (DTT)-treated samples using the indirect antiglobulin test on polyspecific AHG cards.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Statistical Analysis Used:<\/jats:title>\n                    <jats:p>Statistical analyses included Chi-square tests and univariate and multivariate regression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Anti-A titers were significantly higher than anti-B titers for both IgM and IgG. IgG titers were significantly higher than IgM. Plateletpheresis donors and younger individuals exhibited significantly higher isoagglutinin titers. No significant difference was observed based on sex.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Higher isoagglutinin titers among younger and plateletpheresis donors highlight the need for targeted screening to reduce hemolytic risk in ABO-incompatible transfusions. These findings support consideration of donor titer testing and age-based blood component allocation as strategies to enhance transfusion safety.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/aam.aam_217_25","type":"journal-article","created":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T15:00:25Z","timestamp":1764860425000},"page":"869-875","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Analysis of Titers of Immunoglobulin M and Immunoglobulin G ABO Antibodies in Group O Blood and Plateletphersis Donors at a Tertiary Care Hospital"],"prefix":"10.4103","volume":"25","author":[{"given":"Deepika","family":"Aggarwal","sequence":"first","affiliation":[{"name":"Department of Immuno-Hematology and Blood Transfusion, Dayanand Medical College and Hospital, Ludhiana, Punjab, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neetu","family":"Kukar","sequence":"additional","affiliation":[{"name":"Department of Immuno-Hematology and Blood Transfusion, Guru Gobind Singh Medical College and Hospital, Faridkot, Punjab, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anjali","family":"Handa","sequence":"additional","affiliation":[{"name":"Department of Immuno-Hematology and Blood Transfusion, Guru Gobind Singh Medical College and Hospital, Faridkot, Punjab, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunil","family":"Garg","sequence":"additional","affiliation":[{"name":"Department of Surgery, Dr. S.S. Tantia Medical College, Hospital and Research Centre, Sri Ganganagar, Rajasthan, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ram Niwas","family":"Maharishi","sequence":"additional","affiliation":[{"name":"Department of Immuno-Hematology and Blood Transfusion, Adesh Institute of Medical Sciences and Research, Bathinda, Punjab, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,11,26]]},"reference":[{"key":"R1-20260803","doi-asserted-by":"crossref","first-page":"48","DOI":"10.21307\/immunohematology-2019-231","article-title":"The ABO blood group system revisited: A review and update","volume":"25","author":"Storry","year":"2009","journal-title":"Immunohematology"},{"key":"R2-20260803","doi-asserted-by":"crossref","first-page":"259","DOI":"10.5581\/1516-8484.20110073","article-title":"Titers of ABO antibodies in group O blood donors","volume":"33","author":"de Fran\u00e7a","year":"2011","journal-title":"Rev Bras Hematol Hemoter"},{"key":"R5-20260803","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1111\/j.1537-2995.2004.03290.x","article-title":"Significant numbers of apheresis-derived group O platelet units have \u201chigh-titer\u201danti-A\/A, B: Implications for transfusion policy","volume":"44","author":"Josephson","year":"2004","journal-title":"Transfusion"},{"key":"R6-20260803","doi-asserted-by":"crossref","first-page":"223","DOI":"10.3343\/alm.2014.34.3.223","article-title":"Comparison of total and IgG ABO antibody titers in healthy individuals by using tube and column agglutination techniques","volume":"34","author":"Park","year":"2014","journal-title":"Ann Lab Med"},{"key":"R7-20260803","doi-asserted-by":"crossref","first-page":"1186","DOI":"10.1097\/TP.0b013e3181ba07ec","article-title":"Blood group O recipients associated with early graft deterioration in living ABO-incompatible kidney transplantation","volume":"88","author":"Toki","year":"2009","journal-title":"Transplantation"},{"key":"R8-20260803","first-page":"227","article-title":"Comparison of ABO isoagglutinin titers by different tube hamagglutination techniques","volume":"20","author":"Kang","year":"2009","journal-title":"Korean J Blood Transfus"},{"key":"R9-20260803","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1111\/j.1399-3089.2006.00296.x","article-title":"A series of surveys on assay for anti-A\/B antibody by Japanese ABO-incompatible transplantation committee","volume":"13","author":"Kobayashi","year":"2006","journal-title":"Xenotransplantation"},{"key":"R10-20260803","first-page":"110","article-title":"ABO IgG antibody titres of apheresis platelets: A retrospective single center Egyptian study","volume":"12","author":"Farahat","year":"2015","journal-title":"Life Sci J"},{"key":"R11-20260803","first-page":"632","article-title":"Evaluating anti-A and anti-B antibodies titers in 'O'blood group donors for safe transfusion practices","volume":"17","author":"Basnotra","year":"2025","journal-title":"Int J Curr Pharm Rev Res"},{"key":"R12-20260803","doi-asserted-by":"crossref","first-page":"187","DOI":"10.4103\/GJTM.GJTM_38_20","article-title":"Prevalence of high titered anti-A and anti-B antibodies among O bloodgroup individuals and their associated factors","volume":"5","author":"Kannan","year":"2020","journal-title":"Glob J Transfus Med"},{"key":"R13-20260803","doi-asserted-by":"crossref","first-page":"2106","DOI":"10.1111\/j.1537-2995.2008.01814.x","article-title":"Anti-A and anti-B titers in pooled group O platelets are comparable to apheresis platelets","volume":"48","author":"Cooling","year":"2008","journal-title":"Transfusion"},{"key":"R14-20260803","first-page":"89","article-title":"Comparison of IgM and IgG anti-A and anti-B levels in Asian, Caucasion and Negro donors in the North West Thames Region","volume":"59","author":"Redman","year":"1990","journal-title":"Vox Sang"},{"key":"R15-20260803","first-page":"104","article-title":"Antibody Titers Study in Group O Blood Donors: Tube and Column Agglutination Techniques","volume":"2","author":"Sood","year":"2016","journal-title":"J Thrombo Cir"},{"key":"R16-20260803","first-page":"313","article-title":"Comparison of IgG ABO Antibody Titers using Conventional Tube Test and Hydrogel Medium","volume":"25","author":"Thattanon","year":"2015","journal-title":"J Hematol Transfus Med"},{"key":"R17-20260803","first-page":"11436","article-title":"ABo Antibody titer study in healthy donos in tertiary care of Southern Rajasthan","volume":"7","author":"Kumar","year":"2018","journal-title":"IJCAR"},{"key":"R18-20260803","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.bjhh.2016.05.007","article-title":"Dangerous universal donors: the reality of the Hemocentro in Belo Horizonte, Minas Gerais","volume":"38","author":"Godin","year":"2016","journal-title":"Rev Bras Hematol Hemoter"},{"key":"R19-20260803","doi-asserted-by":"crossref","first-page":"250","DOI":"10.5581\/1516-8484.20110067","article-title":"Titers of ABO antibodies in group O blood donors: Patient safety and blood product supply remain a challenge","volume":"33","author":"Denomme","year":"2011","journal-title":"Rev Bras Hematol Hemoter"},{"key":"R20-20260803","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1002\/jcla.21499","article-title":"The Charactersitics of ABO antibodies in group A Thai Donors","volume":"26","author":"Khampanon","year":"2012","journal-title":"J Clin Lab Anal"},{"key":"R21-20260803","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1111\/j.1600-6143.2010.03103.x","article-title":"ABO antibody titer and risk of antibody mediated rejection in ABO incompatible renal transplantation","volume":"10","author":"Tobian","year":"2010","journal-title":"Am J Transplant"},{"key":"R22-20260803","doi-asserted-by":"crossref","first-page":"22","DOI":"10.4103\/0973-6247.200765","article-title":"Antibody titers in group O platelet donors","volume":"11","author":"Tendulkar","year":"2017","journal-title":"Asian J Transfusion Sci"},{"key":"R23-20260803","first-page":"76","article-title":"Evaluation of anti-A and anti-B alloisogglutinin titer in group O plateletphersis donors","volume":"1","author":"Bazigou","year":"2015","journal-title":"Hematol Transfus Int J"}],"container-title":["Annals of African Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/aam.aam_217_25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:50:07Z","timestamp":1787068207000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/aam.aam_217_25"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,26]]},"references-count":21,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.4103\/aam.aam_217_25","relation":{},"ISSN":["1596-3519","0975-5764"],"issn-type":[{"value":"1596-3519","type":"print"},{"value":"0975-5764","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,26]]},"assertion":[{"value":"2025-06-03","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-22","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}