{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:58:59Z","timestamp":1787169539707,"version":"build-2736575974"},"reference-count":17,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,1]]},"abstract":"\n Background:<\/jats:title>\n Replantation is a commonly performed method for avulsed tooth. A vital periodontal membrane (periodontal ligament [PDL]) is significant for the successful healing of replanted teeth. Hence, various storage media are used to preserve the viability of periodontal cells before replantation.<\/jats:p>\n <\/jats:sec>\n \n Objectives:<\/jats:title>\n \n The present study was conducted to evaluate the efficacy of ViaSpan,\n Aloe vera<\/jats:italic>\n , Gatorade solution, and propolis storage media for maintaining the PDL cell viability.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n The present study was conducted on 40 recently extracted teeth which were randomly divided into four study storage groups: Group I: ViaSpan, Group II:\n Aloe vera<\/jats:italic>\n , Group III: Gatorade solution, and Group IV: Propolis. Later they were subjected to centrifugation, and the cells from supernatant were colored with 0.4% trypan blue for determination of viability. The obtained data were statistically evaluated with SPSS package (21.0 version, Inc.; Chicago, IL, USA) using analysis of variance, Mann\u2013Whitney test, and\n Post hoc<\/jats:italic>\n tests.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n The mean viable periodontal cell in Group I was 30.2 cumm, in Group II was 24.6 cumm, Group III was 14.5 cumm, and Group IV in 31.4. The difference was significant (\n P<\/jats:italic>\n < 0.01).\n Post hoc<\/jats:italic>\n test between different groups revealed a significant difference in mean viable periodontal cells (\n P<\/jats:italic>\n < 0.001). Propolis, ViaSpan, and\n Aloe vera<\/jats:italic>\n had higher pH and osmolality values.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n \n This study found that propolis had higher periodontal cell viability followed by ViaSpan solution and\n Aloe vera<\/jats:italic>\n and least in Gatorade solution. Propolis, ViaSpan, and\n Aloe vera<\/jats:italic>\n media can be used as a storage media.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/aam.aam_71_20","type":"journal-article","created":{"date-parts":[[2022,3,18]],"date-time":"2022-03-18T10:25:58Z","timestamp":1647599158000},"page":"34-38","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":4,"title":["An in vitro Evaluation of Efficacy of ViaSpan, Aloe vera, Gatorade Solution, and Propolis Storage Media for Maintaining the Periodontal Ligament Cell Viability"],"prefix":"10.4103","volume":"21","author":[{"given":"Rajat","family":"Misurya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandeep","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prabu Mahin","family":"Syed 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