{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T18:23:19Z","timestamp":1787768599804,"version":"build-2784847793"},"reference-count":15,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T00:00:00Z","timestamp":1773619200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction:<\/jats:title>\n                    <jats:p>\n                      Oral squamous cell carcinoma (OSCC) is a genetically driven malignancy characterized by a high burden of oncogenic mutations that contribute to aggressive tumor behavior, therapeutic resistance, and poor survival outcomes. Conventional treatment modalities largely target downstream molecular pathways without correcting the underlying genetic aberrations, underscoring the need for precision-based therapeutic strategies. This study aimed to assess the feasibility and functional impact of CRISPR-Cas9-mediated gene editing in reversing oncogenic mutations associated with OSCC using an\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      experimental model.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      A controlled\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      experimental study was conducted using the established human OSCC cell lines harboring mutations in key oncogenic genes. Cells were divided into control, mock-transfected, and CRISPR-Cas9\u2013edited groups. Target-specific single-guide RNAs were designed to correct oncogenic mutations using advanced CRISPR-based editing platforms. Gene-editing efficiency was validated by molecular assays, while functional outcomes were assessed using cell proliferation analysis. Statistical evaluation was done using the one-way ANOVA with significance set at\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.05.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      CRISPR-Cas9\u2013edited OSCC cells demonstrated a significant reduction in cell proliferation compared to control and mock-transfected groups (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.001). No significant variation was found between control and mock-transfected cells, confirming minimal procedural influence.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>CRISPR-Cas9-mediated correction of oncogenic mutations effectively suppresses malignant cellular proliferation in OSCC, highlighting its promising role as a precision therapeutic strategy in oral cancer management.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/aam.aam_864_25","type":"journal-article","created":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T15:00:20Z","timestamp":1773673220000},"page":"805-809","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["CRISPR-cas9-Mediated Gene Editing to Reverse Oncogenic Mutations in Oral Squamous Cell Carcinoma"],"prefix":"10.4103","volume":"25","author":[{"given":"Minal","family":"Awinashe","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Diagnostic Science, College of Dentistry, Qassim University, Qassim, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Viswaja","sequence":"additional","affiliation":[{"name":"Department of General Pathology, SRM Dental College, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul Wahab","family":"Pathath","sequence":"additional","affiliation":[{"name":"Department of Clinical Neuroscience, College of Medicine, King Faisal University, Al Ahsa, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fahida P.","family":"Subair","sequence":"additional","affiliation":[{"name":"Kuien\u2019s Total Oral Health Center, Malappuram, Kerala, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anchal","family":"Varshney","sequence":"additional","affiliation":[{"name":"Department of Oral Patholgy and Microbiology, School of Dental Sciences, Manav Rachna Dental College, MRIIRS, Faridabad, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pallavi","family":"Mishra","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, Kalinga Institute of Dental Sciences, KIIT Deemed to be University, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mushir","family":"Mulla","sequence":"additional","affiliation":[{"name":"Department of Public Health, College of Applied Medical Sciences, Qassim University, Qassim, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Munaz","family":"Mulla","sequence":"additional","affiliation":[{"name":"Department of Periodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2026,3,16]]},"reference":[{"key":"R1-20260803","doi-asserted-by":"crossref","first-page":"1383062","DOI":"10.3389\/fonc.2024.1383062","article-title":"Revitalizing oral cancer research: Crispr-Cas9 technology the promise of genetic editing","volume":"14","author":"Sowmya","year":"2024","journal-title":"Front Oncol"},{"key":"R2-20260803","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1126\/science.1225829","article-title":"A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity","volume":"337","author":"Jinek","year":"2012","journal-title":"Science"},{"key":"R3-20260803","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1126\/science.1231143","article-title":"Multiplex genome engineering using CRISPR\/Cas systems","volume":"339","author":"Cong","year":"2013","journal-title":"Science"},{"key":"R4-20260803","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1126\/science.1232033","article-title":"RNA-guided human genome engineering via Cas9","volume":"339","author":"Mali","year":"2013","journal-title":"Science"},{"key":"R5-20260803","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1038\/nature17946","article-title":"Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage","volume":"533","author":"Komor","year":"2016","journal-title":"Nature"},{"key":"R6-20260803","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1038\/nature24644","article-title":"Programmable base editing of A\u2022T to G\u2022C in genomic DNA without DNA cleavage","volume":"551","author":"Gaudelli","year":"2017","journal-title":"Nature"},{"key":"R7-20260803","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1038\/s41586-019-1711-4","article-title":"Search-and-replace genome editing without double-strand breaks or donor DNA","volume":"576","author":"Anzalone","year":"2019","journal-title":"Nature"},{"key":"R8-20260803","doi-asserted-by":"crossref","first-page":"3002","DOI":"10.1158\/0008-5472.CAN-21-2519","article-title":"Efficient correction of oncogenic KRAS and TP53 mutations through CRISPR base editing","volume":"82","author":"Sayed","year":"2022","journal-title":"Cancer Res"},{"key":"R9-20260803","doi-asserted-by":"crossref","first-page":"e57761","DOI":"10.7554\/eLife.57761","article-title":"Genome-wide CRISPR screens of oral squamous cell carcinoma reveal fitness genes in the Hippo pathway","volume":"9","author":"Chai","year":"2020","journal-title":"Elife"},{"key":"R10-20260803","first-page":"704","article-title":"Therapeutic editing of the TP53 gene: Is CRISPR\/Cas9 an Option?Genes (Basel)","volume":"11","author":"Mirgayazova","year":"2020"},{"key":"R11-20260803","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1038\/s41591-020-0840-5","article-title":"Safety and feasibility of CRISPR-edited T cells in patients with refractory non-small-cell lung cancer","volume":"26","author":"Lu","year":"2020","journal-title":"Nat Med"},{"key":"R12-20260803","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s13005-024-00437-x","article-title":"CRISPR\/Cas9-mediated knock out of ITGB6 in human OSCC cells reduced migration and proliferation ability","volume":"20","author":"Geyer","year":"2024","journal-title":"Head Face Med"},{"key":"R13-20260803","doi-asserted-by":"crossref","first-page":"e2411032","DOI":"10.1002\/advs.202411032","article-title":"Targeted CRISPR\/Cas9 lipid nanoparticles elicits therapeutic genome editing in head and neck cancer","volume":"12","author":"Masarwy","year":"2025","journal-title":"Adv Sci (Weinh)"},{"key":"R14-20260803","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1038\/s41392-023-01347-1","article-title":"Targeting p53 pathways: Mechanisms, structures, and advances in therapy","volume":"8","author":"Wang","year":"2023","journal-title":"Signal Transduct Target Ther"},{"key":"R15-20260803","doi-asserted-by":"crossref","first-page":"8572","DOI":"10.1038\/s41467-025-63592-y","article-title":"In vivo CRISPR screening in head and neck cancer reveals Uchl5 as an immunotherapy target","volume":"16","author":"Fu","year":"2025","journal-title":"Nat Commun"}],"container-title":["Annals of African 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History"}},{"value":"2026-02-04","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}