{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:19:20Z","timestamp":1787840360508,"version":"build-2784847793"},"reference-count":40,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      L7\/L12 is a protective antigen conserved in main\n                      <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                      pathogens and is considered as potential vaccine candidate. Outer membrane protein 2b is an immunogen conserved in all\n                      <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                      pathogens.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      The purpose of the current study was to\n                      <jats:italic toggle=\"yes\">in silico<\/jats:italic>\n                      design a L7\/L12-SOmp2b fusion protein and\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      production of the chimera. Two possible fusion forms, L7\/L12-SOmp2b and SOmp2b-L7\/L12, were subjected to\n                      <jats:italic toggle=\"yes\">in silico<\/jats:italic>\n                      modeling and analysis. Cloning and expression of the fusion protein has been done in the pET28a vector and\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      Bl21 (DE3), respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Analysis and validation of the fusion proteins three-dimensional models showed that both models are in the range of native proteins. However, L7\/L12-SOmp2b structure was more valid than the SOmp2b-L7\/L12 model and subjected to\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      production. The major histocompatibility complex II (MHC-II) epitope mapping using Immune Epitope DataBase indicated that the model contained good MHC-II binders. The L7\/L12-Omp2b coding sequence was cloned in pET28a vector. The fusion was successfully expressed in\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      BL21 by induction with isopropyl-\u03b2-d-thiogalactopyranoside. The rL7\/L12-SOmp2b was purified with Ni-NTA column. The yield of the purified rL7\/L12-SOmp2b was estimated by Bradford method to be 240 \u03bcg\/ml of the culture. Western blot analysis revealed a specific reactivity with purified rL7\/L12-SOmp2b produced in\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      cells and showed the expression in the prokaryotic system.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Our data indicates that L7\/L12-SOmp2b fusion protein has a potential to induce both B- and T-cell-mediated immune responses and it can be evaluated as a new subunit vaccine candidate against brucellosis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/abr.abr_10_17","type":"journal-article","created":{"date-parts":[[2018,2,16]],"date-time":"2018-02-16T04:36:02Z","timestamp":1518755762000},"update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["In silico Design, and In vitro Expression of a Fusion Protein Encoding Brucella abortus L7\/L12 and SOmp2b Antigens"],"prefix":"10.4103","volume":"7","author":[{"given":"Maryam","family":"Golshani","sequence":"first","affiliation":[{"name":"From the Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melina","family":"Ghasemian","sequence":"additional","affiliation":[{"name":"Department of Biotechemistry, Qazvin University of Medical Sciences, Qazvin, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nematollah","family":"Gheibi","sequence":"additional","affiliation":[{"name":"Department of Biotechemistry, Qazvin University of Medical Sciences, Qazvin, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeid","family":"Bouzari","sequence":"additional","affiliation":[{"name":"From the Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2018,2,16]]},"reference":[{"key":"R1-20251008","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/S1473-3099(07)70286-4","article-title":"Human brucellosis","volume":"7","author":"Franco","year":"2007","journal-title":"Lancet Infect Dis"},{"key":"R2-20251008","doi-asserted-by":"crossref","first-page":"2","DOI":"10.3389\/fcimb.2012.00002","article-title":"Analyses of Brucella pathogenesis, host immunity, and vaccine targets using systems biology and bioinformatics","volume":"2","author":"He","year":"2012","journal-title":"Front Cell Infect Microbiol"},{"key":"R3-20251008","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/j.vetmic.2009.06.021","article-title":"Brucellosis: A re-emerging zoonosis","volume":"140","author":"Seleem","year":"2010","journal-title":"Vet 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History"}},{"value":"2017-05-01","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}