{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T20:25:16Z","timestamp":1787775916415,"version":"build-2784847793"},"reference-count":26,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,1]]},"abstract":"\n Background:<\/jats:title>\n \n Seidlitzia rosmarinus<\/jats:italic>\n which is commonly called \u201cOshnan\u201d or \u201cEshnan\u201d in Persian belongs to\n Chenopodiaceae<\/jats:italic>\n family. Conventionally, it is believed that this plant is toxic. This study was aimed to evaluate the cytotoxic effect of\n S. rosmarinus<\/jats:italic>\n against HeLa and HepG\n 2<\/jats:sub>\n cell lines.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n S. rosmarinus<\/jats:italic>\n was collected from the desert near Yazd, Iran. Hexane, chloroform, chloroform\/methanol (9:1), and butanol extracts of aerial parts of\n S. rosmarinus<\/jats:italic>\n were prepared. Doxorubicin and dimethyl sulfoxide 10% were used as positive and negative control, respectively. The cytotoxic activity was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n All extracts significantly and concentration dependently reduced viability of HeLa and HepG2 cells. Hexane, chloroform, and butanol extracts at doses of 200, 500, 750, and 1000 \u03bcg\/ml significantly reduced HeLa cell viability (\n P<\/jats:italic>\n < 0.05). Chloroform\/methanol extract at doses of 100\u2013500 \u03bcg\/ml significantly reduced HeLa cell viability (\n P<\/jats:italic>\n < 0.05). Hexane, chloroform, and butanol extracts at doses of 500, 750, and 1000 \u03bcg\/ml significantly reduced HepG2 cell viability (\n P<\/jats:italic>\n < 0.05). Chloroform\/methanol extract at doses of 200, 300, 400, and 500 \u03bcg\/ml significantly reduced HepG cell viability (\n P<\/jats:italic>\n < 0.05). The most cytotoxic extract was chloroform\/methanol extract in both cell lines. Furthermore, in the both cell lines, the second potent extract was chloroform extract.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions:<\/jats:title>\n \n It can be concluded from the findings of this study that\n S. rosmarinus<\/jats:italic>\n is a good candidate for further study to find new cytotoxic agents. Phytochemical investigation on chloroform\/methanol extract and their structures is recommended.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/abr.abr_165_17","type":"journal-article","created":{"date-parts":[[2018,10,24]],"date-time":"2018-10-24T00:44:19Z","timestamp":1540341859000},"update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":3,"title":["Evaluation of Cytotoxic Effect of Different Extracts of Seidlitzia rosmarinus on HeLa and HepG2 Cell Lines"],"prefix":"10.4103","volume":"7","author":[{"given":"Behzad","family":"Zolfaghari","sequence":"first","affiliation":[{"name":"From the Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abbas","family":"Jafarian","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences and Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moein","family":"Rezaei","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences and Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2018,10,24]]},"reference":[{"key":"R1-20251008","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/S0378-8741(00)00341-X","article-title":"Plants used against cancer \u2013 An extension of the work of Jonathan Hartwell","volume":"73","author":"Graham","year":"2000","journal-title":"J Ethnopharmacol"},{"key":"R2-20251008","article-title":"International Agency for Research on Cancer. 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