{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T09:28:37Z","timestamp":1792920517783,"version":"4.5.0"},"reference-count":27,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,1]]},"abstract":"\n Background:<\/jats:title>\n \n Extended-spectrum \u03b2-lactamase (ESBL)-producing is a significant resistant mechanism to \u03b2-lactams in\n Enterobacteriaceae<\/jats:italic>\n , especially in\n Klebsiella pneumoniae<\/jats:italic>\n . The main objectives of this study were to genetically characterize urinary clinical isolates of\n K. pneumoniae<\/jats:italic>\n through the investigating of\n blaTEM<\/jats:italic>\n ,\n blaCTX-M<\/jats:italic>\n and using molecular typing by Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) method. We also determined the frequency of antibiotic resistance of\n K. pneumoniae<\/jats:italic>\n strains to characterize the \u03b2-lactamases included.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n A cross-sectional study was carried out to evaluate 98 strains of\n K. pneumoniae<\/jats:italic>\n isolated from urine culture of outpatients referred to Al-Zahra Hospital, Isfahan, Iran. Antibiotic susceptibility testing was performed using Kirby\u2013Bauer's method. Screening of ESBLs was carried out using double-disk screening test. PCR technique was performed to detect\n TEM<\/jats:italic>\n and\n CTX-M<\/jats:italic>\n genes. The total DNA of each strain was tested by ERIC-PCR.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n In 98\n K. pneumoniae<\/jats:italic>\n studied clinical isolates, 25.5% were ESBL producing and 44.9% multidrug-resistant (MDR). From 25 ESBL isolates, 23 (92%) cases showed MDR phenotype. In ESBL producing isolates, 23 (92%) were\n blaCTX-M<\/jats:italic>\n and 19 (76%)\n blaTEM<\/jats:italic>\n positive. The antimicrobial drug susceptibilities of ESBL isolates indicated high resistant rates for cefotaxime and ceftazidime. All 25 ESBL producing isolates were resistant to cefotaxime. Complex patterns of fingerprints isolates showed that 36% of the isolates were belonged to the cluster no 5.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n This study revealed high antimicrobial resistance rates among ESBL isolates which can lead to various health difficulties. Epidemiological data collection from patients is recommended to develop the strategies to manage antibiotic resistance.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/abr.abr_17_17","type":"journal-article","created":{"date-parts":[[2018,1,29]],"date-time":"2018-01-29T03:43:03Z","timestamp":1517197383000},"update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":21,"title":["Prevalence of CTX-M and TEM \u03b2-lactamases in Klebsiella pneumoniae Isolates from Patients with Urinary Tract Infection, Al-Zahra Hospital, Isfahan, Iran"],"prefix":"10.4103","volume":"7","author":[{"given":"Nafiseh","family":"Maleki","sequence":"first","affiliation":[{"name":"From the Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahra","family":"Tahanasab","sequence":"additional","affiliation":[{"name":"From the Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sina","family":"Mobasherizadeh","sequence":"additional","affiliation":[{"name":"From the Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aliakbar","family":"Rezaei","sequence":"additional","affiliation":[{"name":"From the Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamshid","family":"Faghri","sequence":"additional","affiliation":[{"name":"From the Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2018,1,30]]},"reference":[{"key":"R1-20251008","doi-asserted-by":"crossref","first-page":"e57091","DOI":"10.1371\/journal.pone.0057091","article-title":"Genotypic analysis of Klebsiella pneumoniae isolates in a Beijing Hospital reveals high genetic diversity and clonal population structure of drug-resistant isolates","volume":"8","author":"Wang","year":"2013","journal-title":"PLoS One"},{"key":"R2-20251008","doi-asserted-by":"crossref","first-page":"e27517","DOI":"10.5812\/jjm.27517","article-title":"Multidrug-resistant Escherichia coli and Klebsiella pneumoniae isolated from patients in Kashan, Iran","volume":"8","author":"Moini","year":"2015","journal-title":"Jundishapur J Microbiol"},{"key":"R3-20251008","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.afju.2014.10.004","article-title":"Antimicrobial susceptibility of urinary Klebsiella pneumoniae and the 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\u03b2-lactamase-producing Enterobacteriaceae isolated in Antananarivo, Madagascar","volume":"17","author":"Rakotonirina","year":"2013","journal-title":"BMC Microbiol"},{"key":"R10-20251008","doi-asserted-by":"crossref","first-page":"3724","DOI":"10.1128\/AAC.47.12.3724-3732.2003","article-title":"Prevalence and molecular epidemiology of CTX-M extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae in Russian hospitals","volume":"47","author":"Edelstein","year":"2003","journal-title":"Antimicrob Agents Chemother"},{"key":"R11-20251008","first-page":"126","article-title":"Genetic diversity in clinical isolates of Escherichia coli by enterobacterial repetitive intergenic consensus (ERIC)-PCR technique in Sanandaj hospitals","volume":"5","author":"Ramazanzadeh","year":"2013","journal-title":"Iran J Microbiol"},{"key":"R12-20251008","article-title":"Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing. Twenty Second International Supplement M100. S22. USA Clinical and Laboratory Standards Institute","author":"Cockerill","year":"2012"},{"key":"R13-20251008","doi-asserted-by":"crossref","first-page":"57","DOI":"10.4103\/2277-9175.151872","article-title":"Molecular detection of metallo-\u00df-lactamase gene blaVIM-1 in imipenem-resistant Pseudomonas aeruginosa strains isolated from hospitalized patients in the hospitals of Isfahan","volume":"4","author":"Sedighi","year":"2015","journal-title":"Adv Biomed Res"},{"key":"R14-20251008","first-page":"109","article-title":"Analysis of amino acid substitution mutations of gyrA and parC genes in clonal lineage of Klebsilla pneumoniae conferring high-level quinolone resistance","volume":"2","author":"Norouzi","year":"2014","journal-title":"J Med Microbiol Infect Dis"},{"key":"R15-20251008","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1128\/CMR.8.4.557","article-title":"Beta-lactamases in laboratory and clinical 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marcescens","volume":"11","author":"Knothe","year":"1983","journal-title":"Infection"},{"key":"R22-20251008","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1055\/s-2003-37923","article-title":"Global epidemiology of antimicrobial resistance among community-acquired and nosocomial pathogens: A five-year summary from the SENTRY Antimicrobial Surveillance Program (1997-2001)","volume":"24","author":"Jones","year":"2003","journal-title":"Semin Respir Crit Care Med"},{"key":"R23-20251008","doi-asserted-by":"crossref","first-page":"e8256","DOI":"10.5812\/jjm.8256","article-title":"Prevalence of Klebsiella pneumoniae encoding genes for Ctx-M-1, Tem-1 and Shv-1 extended-spectrum beta lactamases (ESBL) enzymes in clinical specimens","volume":"6","author":"Khosravi","year":"2013","journal-title":"Jundishapur J Microbiol"},{"issue":"Suppl 1","key":"R24-20251008","first-page":"S41","article-title":"Frequency assessment of \u00df-lactamase enzymes in Escherichia coli and Klebsiella 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