{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:45:56Z","timestamp":1787071556960,"version":"build-2736575974"},"reference-count":25,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"abstract":"\n Background:<\/jats:title>\n \n Candidiasis is one of the most common fungal infections caused by\n Candida<\/jats:italic>\n species\n .<\/jats:italic>\n Due to increasing reports of various side effects of current antifungals and the emergence of resistant isolates, other therapeutic tools seem necessary. Recently, researchers focused on herbal medicines with fewer side effects and lower costs. This study aimed to evaluate the antifungal susceptibility and toxicity profiles of dry extract of\n Crocus sativus<\/jats:italic>\n petals in\n C. albicans<\/jats:italic>\n isolates from patients with proven candidiasis.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n Fifty clinical isolates of\n C. albicans<\/jats:italic>\n were gathered, cultured, and stored. After preparing and purifying the dry extract of\n C. sativus<\/jats:italic>\n petals, the cytotoxicity test was performed by MTT assay in the L929 cell line. Antifungal susceptibility testing of dry extract of\n C. sativus<\/jats:italic>\n petals and fluconazole was performed using the broth microdilution according to the CLSI-M27-S3 document. Data were analyzed using SPSS version 27 software.\n P<\/jats:italic>\n < 0.05 was considered as a significant statistical value.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n The minimum inhibitory concentrations 50 and geometric mean of fluconazole and dry extract of\n C. sativus<\/jats:italic>\n petals for all isolates were (0.25 \u00b5g\/mL and 0.19 \u00b5g\/mL) and (4 \u00b5g\/mL and 5.32 \u00b5g\/mL), respectively. These values were statistically different (\n P<\/jats:italic>\n < 0.05). The dry extract of\n C. sativus<\/jats:italic>\n petals had lower antifungal activity against\n C. albicans<\/jats:italic>\n than fluconazole.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions:<\/jats:title>\n \n We found that the antifungal activity of the dry extract of\n C. sativus<\/jats:italic>\n petals is lower than that of fluconazole; however, its safety can be considered a potential therapeutic option for treating different forms of candidiasis.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/abr.abr_513_24","type":"journal-article","created":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T08:01:17Z","timestamp":1759132877000},"update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the Cytotoxicity and Antifungal Efficacy of Crocus sativus (saffron) Petals on Clinical Isolates of Candida albicans"],"prefix":"10.4103","volume":"14","author":[{"given":"Marziye","family":"Esmaeilpour Jouneghani","sequence":"first","affiliation":[{"name":"Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid","family":"Morovati","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shima","family":"Gharibi","sequence":"additional","affiliation":[{"name":"Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vajiheh","family":"Azimian Zavareh","sequence":"additional","affiliation":[{"name":"Department of Plant and Animal Biology, Faculty of Biological Sciences and Technology, University of Isfahan, Isfahan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akbar","family":"Hoseinnejad","sequence":"additional","affiliation":[{"name":"Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran"},{"name":"Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vajiheh","family":"Nazemi","sequence":"additional","affiliation":[{"name":"Faculty of Agricultural Engineering-Food Science and Technology, Islamic Azad University of Khorasgan, Isfahan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasoul","family":"Mohammadi","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology and Mycology, School of Medicine, Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, 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