{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T18:35:29Z","timestamp":1793212529290,"version":"4.5.0"},"reference-count":32,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Mesenchymal stromal cells (MSCs) are among the most extensively studied candidates for cell-based therapy. The homing capacity of MSCs is a critical factor influencing the efficacy of MSC-based therapies. This study aimed to investigate the homing of placenta-derived mesenchymal stromal cells (PD-MSCs) in various damaged tissues of immunodeficient Cg-PrkdcscidIl2rgtm1Sug\/ShiJic (NOG) mice in response to total body irradiation (TBI).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      NOG mice were irradiated with 3.5 Gy. PD-MSCs were isolated, characterized, and transduced with green fluorescent protein (GFP). Twenty-four hours after irradiation, 250 \u00d7 10\n                      <jats:sup>3<\/jats:sup>\n                      GFP-PD-MSCs were injected intravenously through the tail vein in the intervention group, while the control group received phosphate-buffered saline. Peripheral blood, bone marrow, spleen, lungs, liver, intestine, and kidneys were collected at two time points (2 and 14 days postexposure). Samples were analyzed using flow cytometry, polymerase chain reaction (PCR), and immunohistochemistry (IHC).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>A weak band was detected in some PCR samples following GFP-PD-MSCs injection; however, the presence of these cells was not confirmed by flow cytometry or IHC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Intravenous infusion of GFP-PD-MSCs did not result in detectable homing to various damaged tissues in response to TBI. This suggests that the inability of these cells to home effectively may limit their therapeutic potential.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/abr.abr_536_23","type":"journal-article","created":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T18:00:11Z","timestamp":1793210411000},"update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Homing Assessment of Human Placenta-Derived Mesenchymal Stromal Cells in Immunodeficient NOG Mice Following Sublethal Irradiation"],"prefix":"10.4103","volume":"15","author":[{"given":"Nasser","family":"Ahmadbeigi","sequence":"first","affiliation":[{"name":"Gene Therapy Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samad","family":"Muhammadnejad","sequence":"additional","affiliation":[{"name":"Gene Therapy Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahad","family":"Muhammadnejad","sequence":"additional","affiliation":[{"name":"Cancer Biology Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Vasei","sequence":"additional","affiliation":[{"name":"Cell-Based Therapies Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kobra","family":"Moradzadeh","sequence":"additional","affiliation":[{"name":"Gene Therapy Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javad","family":"Verdi","sequence":"additional","affiliation":[{"name":"Department of Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Kavianpour","sequence":"additional","affiliation":[{"name":"Department of Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran"},{"name":"Department of Tissue Engineering and Applied Cell Sciences, School of Medicine, Qom University of Medical Sciences, Qom, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2026,4]]},"reference":[{"key":"R1-20261028","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2016\/6940283","article-title":"Stem cells applications in regenerative medicine and disease therapeutics","volume":"1","author":"Mahla","year":"2016","journal-title":"Int J Cell Biol"},{"key":"R2-20261028","first-page":"1","article-title":"Mesenchymal stromal cells induce inhibitory effects on hepatocellular carcinoma through various signaling pathways","volume":"1","author":"Ai","year":"2019","journal-title":"Cancer Cell Int"},{"key":"R3-20261028","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1038\/nature00870","article-title":"Pluripotency of mesenchymal stem cells derived from adult marrow","volume":"418","author":"Jiang","year":"2002","journal-title":"Nature"},{"key":"R4-20261028","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1634\/stemcells.2005-0370","article-title":"Mesenchymal stem cells spontaneously express neural proteins in culture and are neurogenic after transplantation","volume":"24","author":"Deng","year":"2006","journal-title":"Stem cells"},{"key":"R5-20261028","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1161\/CIRCRESAHA.113.301202","article-title":"Bone-derived stem cells repair the heart after myocardial infarction through transdifferentiation and paracrine signaling mechanisms","volume":"113","author":"Duran","year":"2013","journal-title":"Circ Res"},{"key":"R6-20261028","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.expneurol.2020.113456","article-title":"Exosomes derived from bone marrow mesenchymal stem cells harvested from type two diabetes rats promotes neurorestorative effects after stroke in type two diabetes rats","volume":"334","author":"Venkat","year":"2020","journal-title":"Exp Neurol"},{"key":"R7-20261028","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1002\/sctm.19-0380","article-title":"Combination product of dermal matrix, human mesenchymal stem cells, and timolol promotes diabetic wound healing in mice","volume":"9","author":"Yang","year":"2020","journal-title":"Stem Cells Transl Med"},{"key":"R8-20261028","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1016\/j.orthres.2004.02.012","article-title":"Mesenchymal stem cells used for rabbit tendon repair can form ectopic bone and express alkaline phosphatase activity in constructs","volume":"22","author":"Harris","year":"2004","journal-title":"J Orthop Res"},{"key":"R9-20261028","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2019\/9628536","article-title":"Challenges and controversies in human mesenchymal stem cell therapy","volume":"18","author":"Lukomska","year":"2019","journal-title":"Stem Cells Int"},{"key":"R10-20261028","doi-asserted-by":"crossref","first-page":"937","DOI":"10.2174\/1574888X17666220831104747","article-title":"Challenges in mesenchymal stromal cell-based therapies","volume":"18","author":"Kavianpour","year":"2022","journal-title":"Curr Stem Cell Res Ther"},{"key":"R11-20261028","first-page":"1","article-title":"The Therapeutic potential of mesenchymal stromal cells for regenerative medicine: Current knowledge and future understandings","volume":"18","author":"Merimi","year":"2021","journal-title":"Front Cell Dev Biol"},{"key":"R12-20261028","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1002\/stem.3242","article-title":"Stem cell homing: from physiology to therapeutics","volume":"38","author":"Liesveld","year":"2020","journal-title":"Stem Cells"},{"key":"R13-20261028","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/cells9051145","article-title":"Identifying the therapeutic significance of mesenchymal stem cells","volume":"9","author":"Mishra","year":"2020","journal-title":"Cells"},{"key":"R14-20261028","first-page":"15","article-title":"Long-term quantitative biodistribution and side effects of human mesenchymal stem cells (hMSCs) engraftment in NOD\/SCID mice following irradiation","volume":"1","author":"Fran\u00e7ois","year":"2014","journal-title":"Stem Cells Int"},{"key":"R15-20261028","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1053\/j.gastro.2015.03.013","article-title":"Mesenchymal stem cells reduce colitis in mice via release of TSG6, independently of their localization to the intestine","volume":"149","author":"Sala","year":"2015","journal-title":"Gastroenterology"},{"key":"R16-20261028","doi-asserted-by":"crossref","first-page":"2969","DOI":"10.1089\/scd.2011.0588","article-title":"Genetic modification of mesenchymal stem cells to overexpress CXCR4 and CXCR7 does not improve the homing and therapeutic potentials of these cells in experimental acute kidney injury","volume":"21","author":"Gheisari","year":"2012","journal-title":"Stem Cells Dev"},{"key":"R17-20261028","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.isci.2019.05.004","article-title":"Mesenchymal stromal cell homing: mechanisms and strategies for improvement","volume":"15","author":"Ullah","year":"2019","journal-title":"iScience"},{"key":"R18-20261028","doi-asserted-by":"crossref","first-page":"1362","DOI":"10.1002\/jcb.29371","article-title":"Effects of human placenta-derived mesenchymal stem cells with NK4 gene expression on glioblastoma multiforme cell lines","volume":"121","author":"Jabbarpour","year":"2020","journal-title":"J Cell Biochem"},{"key":"R19-20261028","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.mcp.2010.10.003","article-title":"Genomic DNA extraction from whole blood stored from 15-to 30-years at - 20 C by rapid phenol\u2013chloroform protocol: A useful tool for genetic epidemiology studies","volume":"25","author":"Di Pietro","year":"2011","journal-title":"Mol Cell Probes"},{"key":"R20-20261028","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.1016\/j.bbmt.2013.07.025","article-title":"Delayed marrow infusion in mice enhances hematopoietic and osteopoietic engraftment by facilitating transient expansion of the osteoblastic niche","volume":"19","author":"Marino","year":"2013","journal-title":"Biol Blood Marrow Transplant"},{"key":"R21-20261028","doi-asserted-by":"crossref","first-page":"297","DOI":"10.3389\/fimmu.2012.00297","article-title":"Mesenchymal stem cells are short-lived and do not migrate beyond the lungs after intravenous infusion","volume":"3","author":"Eggenhofer","year":"2012","journal-title":"Front Immunol"},{"key":"R22-20261028","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1007\/978-1-61779-468-1_15","article-title":"Intravenous human mesenchymal stem cells transplantation in NOD\/SCID mice preserve liver integrity of irradiation damage","volume":"826","author":"Mouiseddine","year":"2012","journal-title":"Methods Mol Biol"},{"key":"R23-20261028","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1159\/000353233","article-title":"Human unrestricted somatic stem cell administration fails to protect nude mice from cisplatin-induced acute kidney injury","volume":"123","author":"Gheisari","year":"2013","journal-title":"Nephron Exp Nephrol"},{"key":"R24-20261028","first-page":"1","article-title":"Fate and effect of intravenously infused mesenchymal stem cells in a mouse model of hepatic ischemia reperfusion injury and resection","volume":"11","author":"Saat","year":"2016","journal-title":"Stem Cells Int"},{"key":"R25-20261028","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1161\/01.CIR.0000084828.50310.6A","article-title":"Systemic delivery of bone marrow\u2013derived mesenchymal stem cells to the infarcted myocardium: Feasibility, cell migration, and body distribution","volume":"108","author":"Barbash","year":"2003","journal-title":"Circulation"},{"key":"R26-20261028","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1161\/CIRCULATIONAHA.105.537480","article-title":"Dynamic imaging of allogeneic mesenchymal stem cells trafficking to myocardial infarction","volume":"112","author":"Kraitchman","year":"2005","journal-title":"Circulation"},{"key":"R27-20261028","doi-asserted-by":"crossref","first-page":"219","DOI":"10.3727\/096368909X479677","article-title":"Time-dependent migration of systemically delivered bone marrow mesenchymal stem cells to the infarcted heart","volume":"19","author":"Assis","year":"2010","journal-title":"Cell Transplant"},{"key":"R28-20261028","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1016\/j.surg.2014.05.008","article-title":"Human adipose\u2013derived mesenchymal stem cells attenuate liver ischemia\u2013reperfusion injury and promote liver regeneration","volume":"156","author":"Saidi","year":"2014","journal-title":"Surgery"},{"key":"R29-20261028","doi-asserted-by":"crossref","first-page":"160","DOI":"10.3390\/ijms242216040","article-title":"Mesenchymal stem cells in the pathogenesis and therapy of autoimmune and autoinflammatory diseases","volume":"24","author":"Zaripova","year":"2023","journal-title":"Int J Mol Sci"},{"key":"R30-20261028","doi-asserted-by":"crossref","first-page":"1994","DOI":"10.1074\/jbc.M114.605063","article-title":"CXCR4 receptor overexpression in mesenchymal stem cells facilitates treatment of acute lung injury in rats","volume":"290","author":"Yang","year":"2015","journal-title":"J Biol Chem"},{"key":"R31-20261028","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15761\/JSCRM.1000127","article-title":"The known molecules involved in MSC homing and migration","volume":"3","author":"Ghaffari-Nazari","year":"2018","journal-title":"J Stem Cell Res Med"},{"key":"R32-20261028","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13287-018-1031-x","article-title":"LincRNA-p21 promotes mesenchymal stem cell migration capacity and survival through hypoxic preconditioning","volume":"9","author":"Meng","year":"2018","journal-title":"Stem Cell Res Ther"}],"container-title":["Advanced Biomedical Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/abr.abr_536_23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T18:00:17Z","timestamp":1793210417000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/abr.abr_536_23"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":32,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.4103\/abr.abr_536_23","relation":{},"ISSN":["2277-9175"],"issn-type":[{"value":"2277-9175","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"2023-12-28","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-10","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}