{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:46:02Z","timestamp":1787071562704,"version":"build-2736575974"},"reference-count":28,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"abstract":"\n Background:<\/jats:title>\n \n Candida<\/jats:italic>\n species are opportunistic pathogens that can cause local or systemic invasive infections in patients hospitalized in intensive care units (ICUs). The present study aimed to rapidly and precisely identify\n Candida<\/jats:italic>\n species, especially\n Candida auris<\/jats:italic>\n , from patients hospitalized in high-risk units using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n In this study, 136 clinical samples were prospectively collected from different organs of patients hospitalized in high-risk units recruited from three tertiary care training hospitals in Isfahan. Direct microscopic analysis using potassium hydroxide 10% followed by culturing on Sabouraud dextrose agar and CHROMagar culture media was primarily used to screen\n Candida<\/jats:italic>\n species. Molecular detection of clinical isolates was mainly done using the PCR-RFLP method. Then, all the isolates were subjected to confirmatory diagnosis again through amplification of the\n C. auris<\/jats:italic>\n -specific PCR.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n Candida albicans<\/jats:italic>\n was the most prevalent isolate (48.8%), followed by\n Candida glabrata<\/jats:italic>\n (17.6%),\n Candida tropicalis<\/jats:italic>\n (16.1%),\n Candida parapsilosis<\/jats:italic>\n (6.6%),\n Candida krusei<\/jats:italic>\n (5.8%),\n Candida famata<\/jats:italic>\n (4.4%),\n Candida kefyr<\/jats:italic>\n (2.9%), and\n Candida guilliermondii<\/jats:italic>\n (1.4%). Therefore, the prevalence of non-albicans\n Candida<\/jats:italic>\n species was higher than\n C. albicans<\/jats:italic>\n isolates. No\n C. auris<\/jats:italic>\n isolates were identified among 136 patients. However, according to the positive control,\n C. auris<\/jats:italic>\n had no restriction enzyme cleavage site for\n Msp<\/jats:italic>\n I, and its PCR and digestion products were the same size (401 bp).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions:<\/jats:title>\n \n The simple and reliable PCR-RFLP assay used in the study has the potential to identify rare yeasts such as\n C. auris<\/jats:italic>\n . It can reduce turnaround times and costs if applied in developing countries.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/abr.abr_678_24","type":"journal-article","created":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T08:00:37Z","timestamp":1759132837000},"update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Identification of Candida Species among Iranian Patients: Pursuing Candida auris"],"prefix":"10.4103","volume":"14","author":[{"given":"Fatemeh","family":"Safari","sequence":"first","affiliation":[{"name":"Department of Microbiology, Fal.C., Islamic Azad University, Isfahan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahboobeh","family":"Madani","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Fal.C., Islamic Azad University, Isfahan, 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