{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:20:07Z","timestamp":1787761207994,"version":"build-2784847793"},"reference-count":27,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,1]]},"abstract":"\n Background:<\/jats:title>\n \n In this study, the effects of methadone and naloxone on the expression of toll-like receptor 4 (\n TLR4<\/jats:italic>\n ) gene have been evaluated in human non-small cell lung carcinoma A549 cell line migration using\n in-silico<\/jats:italic>\n and\n in vitro<\/jats:italic>\n techniques.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n Lung cancer A549 cell cultures were stimulated for 24 h with methadone (5, 10, and 20 \u03bcM) and naloxone (20 and 40 \u03bcM) concentrations. The level of\n TLR4<\/jats:italic>\n expression was determined by the quantitative real-time polymerase chain reaction. Migration of the A549 cells was investigated after a 4-h incubation period with methadone using the Boyden Chamber assay.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n Migration rate of the A549 cells treated with 5 (\n P<\/jats:italic>\n < 0.05) and 20 (\n P<\/jats:italic>\n < 0.01) \u03bcM methadone was, respectively, increased and decreased with 20 \u03bcM naloxone (\n P<\/jats:italic>\n < 0.05). Furthermore, the\n TLR4<\/jats:italic>\n expression was enhanced with 5 (\n P<\/jats:italic>\n < 0.05) and 20 (\n P<\/jats:italic>\n < 0.01) \u03bcM methadone and decreased with 20 (\n P<\/jats:italic>\n < 0.05) and 40 \u03bcM naloxone (\n P<\/jats:italic>\n < 0.01). In addition,\n in silico<\/jats:italic>\n docking analysis revealed docking of methadone to MD-2 and\n TLR4<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n \n According to the present DATA, methadone affects the\n TLR4<\/jats:italic>\n expression. It may however cause adverse consequences by increasing the\n TLR4<\/jats:italic>\n expression. Therefore, the useful analgesic properties of methadone should be separated from the unwanted\n TLR4<\/jats:italic>\n -mediated side effects.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/abr.abr_97_21","type":"journal-article","created":{"date-parts":[[2022,12,26]],"date-time":"2022-12-26T04:53:43Z","timestamp":1672030423000},"page":"122","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":4,"title":["Effects of Methadone on the Toll-like Receptor 4 Expression in Human Non-Small Cell Lung Carcinoma A549 Cell Line Using In-silico and In vitro 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