{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T20:25:18Z","timestamp":1787775918606,"version":"build-2784847793"},"reference-count":27,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      Marine organisms produce a variety of compounds with pharmacological activities, including anticancer effects. They contain several secondary metabolites with interesting biological activities. This study attempted to find cytotoxicity of Hexane, Dichloromethane and Butanol partitions of\n                      <jats:italic toggle=\"yes\">Holothuria leucospilota<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Echinometra mathaei<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">H. leucospilota<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">E. mathaei<\/jats:italic>\n                      were collected from Persian Gulf. The animals were extracted by maceration with methanol-ethyl acetate (1:1). The\n                      <jats:italic toggle=\"yes\">H. leucospilota<\/jats:italic>\n                      extract was partitioned by Kupchan method to hexane, dichloromethane, butanol, and water partitions. The cytotoxic activity of the extracts was investigated against HeLa (cervical cancer) and human umbilical vein endothelial cells cell lines by mitochondrial tetrazolium test assay after 72 h.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The cell survivals of HeLa cell were decreased by increasing the concentration of extracts. A significant reduction in cell viability at the doses of 30 (\u03bcg\/ml) of dichloromethane (DCM) partition, 0.3, 3, and 30 (\u03bcg\/ml) of ButOH partitions of sea cucumber, and 0.5 (\u03bcg\/ml) of\n                      <jats:italic toggle=\"yes\">E. mathaei<\/jats:italic>\n                      was observed. The median growth inhibitory concentration value of Hex, DCM, ButoH, and water partitions were 0.301, 0.21, 2.29, and 0.229 \u03bcg\/ml, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      This study reveals that different partitions of\n                      <jats:italic toggle=\"yes\">H. leucospilota<\/jats:italic>\n                      and total extract of\n                      <jats:italic toggle=\"yes\">E. mathaei<\/jats:italic>\n                      have cytotoxic activity against cancer cell lines. More study is necessary to find the active metabolites in the more active partitions.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/abr.abr_9_18","type":"journal-article","created":{"date-parts":[[2018,7,2]],"date-time":"2018-07-02T03:33:15Z","timestamp":1530502395000},"update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":4,"title":["Cytotoxic Screening of Marine Organisms from Persian Gulf"],"prefix":"10.4103","volume":"7","author":[{"given":"Golnaz","family":"Vaseghi","sequence":"first","affiliation":[{"name":"From the Isfahan Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farideh","family":"Hajakbari","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences and Isfahan Pharmacy Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shabnam","family":"Sajjadi","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasim","family":"Dana","sequence":"additional","affiliation":[{"name":"Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences Isfahan, Isfahan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad","family":"Ghasemi","sequence":"additional","affiliation":[{"name":"Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences Isfahan, Isfahan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Afsaneh","family":"Yegdaneh","sequence":"additional","affiliation":[{"name":"Department of 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