{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T13:29:20Z","timestamp":1790429360869,"version":"4.1.0"},"reference-count":41,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Azoospermia patients who carry a monogenetic mutation that causes meiotic arrest may have their biological child through genetic correction in spermatogonial stem cells (SSCs). However, such therapy for infertility has not been experimentally investigated yet. In this study, a mouse model with an X-linked testis-expressed 11 (\n                      <jats:italic toggle=\"yes\">TEX11<\/jats:italic>\n                      ) mutation (\n                      <jats:italic toggle=\"yes\">Tex11<\/jats:italic>\n                      <jats:sup>PM\/Y<\/jats:sup>\n                      ) identified in azoospermia patients exhibited meiotic arrest due to aberrant chromosome segregation.\n                      <jats:italic toggle=\"yes\">Tex11<\/jats:italic>\n                      <jats:sup>PM\/Y<\/jats:sup>\n                      SSCs could be isolated and expanded\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      normally, and the mutation was corrected by clustered regularly interspaced short palindromic repeats (CRISPR)\u2013CRISPR-associated endonuclease 9 (Cas9), leading to the generation of repaired SSC lines. Whole-genome sequencing demonstrated that the mutation rate in repaired SSCs is comparable with that of autonomous mutation in untreated\n                      <jats:italic toggle=\"yes\">Tex11<\/jats:italic>\n                      <jats:sup>PM\/Y<\/jats:sup>\n                      SSCs, and no predicted off-target sites are modified. Repaired SSCs could restore spermatogenesis in infertile males and give rise to fertile offspring at a high efficiency. In summary, our study establishes a paradigm for the treatment of male azoospermia by combining\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      expansion of SSCs and gene therapy.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/aja.aja_97_20","type":"journal-article","created":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T06:41:22Z","timestamp":1612248082000},"page":"590-599","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":44,"title":["Rescue of male infertility through correcting a genetic mutation causing meiotic arrest in spermatogonial stem 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