{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T16:15:12Z","timestamp":1791476112344,"version":"4.3.4"},"reference-count":41,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:sec>\n                    <jats:title\/>\n                    <jats:p>We investigated the therapeutic effects of superoxide dismutase (SOD) from thermophilic bacterium HB27 on chronic prostatitis\/chronic pelvic pain syndrome (CP\/CPPS) and its underlying mechanisms. A Sprague\u2013Dawley rat model of CP\/CPPS was prepared and then administered saline or Thermus thermophilic (Tt)-SOD intragastrically for 4 weeks. Prostate inflammation and fibrosis were analyzed by hematoxylin and eosin staining, and Masson staining. Alanine transaminase (ALT), aspartate transaminase (AST), serum creatinine (CR), and blood urea nitrogen (BUN) levels were assayed for all animals. Enzyme-linked immunosorbent assays (ELISA) were performed to analyze serum cytokine concentrations and tissue levels of malondialdehyde, nitric oxide, SOD, catalase, and glutathione peroxidase. Reactive oxygen species levels were detected using dichlorofluorescein diacetate. The messenger ribonucleic acid (mRNA) expression of tissue cytokines was analyzed by reverse transcription polymerase chain reaction (RT-PCR), and infiltrating inflammatory cells were examined using immunohistochemistry. Nuclear factor-\u03baB (NF-\u03baB) P65, P38, and inhibitor of nuclear factor-\u03baB\u03b1 (I-\u03baB\u03b1) protein levels were determined using western blot. Tt-SOD significantly improved histopathological changes in CP\/CPPS, reduced inflammatory cell infiltration and fibrosis, increased pain threshold, and reduced the prostate index. Tt-SOD treatment showed no significant effect on ALT, AST, CR, or BUN levels. Furthermore, Tt-SOD reduced inflammatory cytokine expression in prostate tissue and increased antioxidant capacity. This anti-inflammatory activity correlated with decreases in the abundance of cluster of differentiation 3 (CD3), cluster of differentiation 45 (CD45), and macrophage inflammatory protein 1\u03b1 (MIP1\u03b1) cells. Tt-SOD alleviated inflammation and oxidative stress by reducing NF-\u03baB P65 and P38 protein levels and increasing I-\u03baB\u03b1 protein levels. These findings support Tt-SOD as a potential drug for CP\/CPPS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/aja202157","type":"journal-article","created":{"date-parts":[[2021,11,2]],"date-time":"2021-11-02T13:09:19Z","timestamp":1635858559000},"page":"323-331","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":11,"title":["Effect of thermophilic bacterium HB27 manganese superoxide dismutase in a rat model of chronic prostatitis\/chronic pelvic pain syndrome 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