{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T15:20:57Z","timestamp":1790349657236,"version":"4.1.0"},"reference-count":33,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:p>\n                    The present study aimed to evaluate the clinical outcomes of magnetic-activated cell sorting (MACS) in sperm preparation for male subjects with a sperm DNA fragmentation index (DFI) \u226530%. A total of 86 patients who had undergone their first long-term long protocol were selected. The protocol involved\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles, and the patients were divided into the MACS or control groups. The MACS group included sperm samples analyzed with MACS that were combined with density gradient centrifugation (DGC) and the swim-up (SU) technique (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 39), and the control group included sperm samples prepared using standard techniques (DGC and SU;\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 41). No differences were noted with regard to basic clinical characteristics, number of oocytes retrieved, normal fertilization rate, cleavage rate, or transplantable embryo rate between the two groups in IVF\/ICSI. In addition, the clinical pregnancy and implantation rates of the first embryo transfer cycles indicated no significant differences between the two groups. However, there was a tendency to improve the live birth rate (LBR) of the first embryo transfer cycle (63.2%\n                    <jats:italic toggle=\"yes\">vs<\/jats:italic>\n                    53.9%) and the cumulative LBR (79.5%\n                    <jats:italic toggle=\"yes\">vs<\/jats:italic>\n                    70.7%) in the MACS group compared with the control group. Moreover, the number of transferred embryos (mean \u00b1 standard deviation [s.d.]: 1.7 \u00b1 0.7\n                    <jats:italic toggle=\"yes\">vs<\/jats:italic>\n                    2.3 \u00b1 1.6) and the transfer number of each retrieved cycle (mean \u00b1 s.d.: 1.2 \u00b1 0.5\n                    <jats:italic toggle=\"yes\">vs<\/jats:italic>\n                    1.6 \u00b1 0.8) were significantly lower in the MACS group than those in the control group. Thus, the selection of nonapoptotic spermatozoa by MACS for higher sperm DFI could improve assisted reproductive clinical outcomes.\n                  <\/jats:p>","DOI":"10.4103\/aja202161","type":"journal-article","created":{"date-parts":[[2021,10,22]],"date-time":"2021-10-22T02:22:45Z","timestamp":1634869365000},"page":"367-372","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":21,"title":["Magnetic-activated cell sorting of nonapoptotic spermatozoa with a high DNA fragmentation index improves the live birth rate and decreases transfer cycles of IVF\/ICSI"],"prefix":"10.4103","volume":"24","author":[{"given":"Jie","family":"Mei","sequence":"first","affiliation":[{"name":"Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China"},{"name":"Center for Molecular Reproductive Medicine, Nanjing University, Nanjing 210008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin-Jun","family":"Chen","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China"},{"name":"Center for Molecular Reproductive Medicine, Nanjing University, Nanjing 210008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin-Xin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Andrology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing-Qiang","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Andrology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai-Xiang","family":"Sun","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China"},{"name":"Center for Molecular Reproductive Medicine, Nanjing University, Nanjing 210008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Jun","family":"Ding","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China"},{"name":"Center for Molecular Reproductive Medicine, Nanjing University, Nanjing 210008, China"},{"name":"Clinical Center for Stem Cell Research, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-Xia","family":"Wang","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2021,10,22]]},"reference":[{"key":"-20240829","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.rbmo.2012.09.019","article-title":"Sperm DNA damage has a negative association with live birth rates after IVF","volume":"26","author":"Simon","year":"2013","journal-title":"Reprod Biomed Online"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1016\/j.fertnstert.2003.09.063","article-title":"Sperm chromatin structure assay (SCSA) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection cycles","volume":"81","author":"Virro","year":"2004","journal-title":"Fertil Steril"},{"key":"-20240829","first-page":"836","article-title":"CSRM consensus on key indicators for quality control in IVF laboratory","volume":"27","author":"Sun","year":"2018","journal-title":"Reprod Med"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1002\/j.1939-4640.2000.tb03284.x","article-title":"Semen quality and human fertility: a prospective study with healthy couples","volume":"21","author":"Zinaman","year":"2000","journal-title":"J Androl"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/978-1-62703-038-0_1","article-title":"Methods for sperm concentration determination","volume":"927","author":"Bjorndahl","year":"2013","journal-title":"Methods Mol Biol"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/and.12003","article-title":"Effects of oral antioxidant treatment upon the dynamics of human sperm DNA fragmentation and subpopulations of sperm with highly degraded DNA","volume":"45","author":"Abad","year":"2013","journal-title":"Andrologia"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1016\/j.rbmo.2014.02.004","article-title":"Sperm DNA damage caused by oxidative stress: modifiable clinical, lifestyle and nutritional factors in male infertility","volume":"28","author":"Wright","year":"2014","journal-title":"Reprod Biomed Online"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1038\/aja.2011.133","article-title":"Sperm preparation: state-of-the-art\u2013physiological aspects and application of advanced sperm preparation methods","volume":"14","author":"Henkel","year":"2012","journal-title":"Asian J Androl"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.rbmo.2013.09.019","article-title":"High-magnification selection of spermatozoa prior to oocyte injection: confirmed and potential indications","volume":"28","author":"Boitrelle","year":"2014","journal-title":"Reprod Biomed Online"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"2409","DOI":"10.1093\/humrep\/dep088","article-title":"Selection of sperm based on combined density gradient and may improve ICSI outcome","volume":"24","author":"Kheirollahi-Kouhestani","year":"2009","journal-title":"Hum Reprod"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"2646","DOI":"10.1093\/humrep\/den330","article-title":"Prospective controlled trial of an electrophoretic method of sperm preparation for assisted reproduction: comparison with density gradient centrifugation","volume":"23","author":"Fleming","year":"2008","journal-title":"Hum Reprod"},{"issue":"Suppl 3","key":"-20240829","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S1472-6483(10)60282-9","article-title":"Sperm-hyaluronan-binding assay: clinical value in conventional IVF under Italian law","volume":"19","author":"Tarozzi","year":"2009","journal-title":"Reprod Biomed Online"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1093\/humrep\/dey239","article-title":"Microfluidic sorting selects sperm for clinical use with reduced DNA damage compared to density gradient centrifugation with swim-up in split semen samples","volume":"33","author":"Quinn","year":"2018","journal-title":"Hum Reprod"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s10815-014-0370-5","article-title":"DNA fragmentation in human sperm after magnetic-activated cell sorting","volume":"32","author":"Bucar","year":"2015","journal-title":"J Assist Reprod Genet"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1016\/S0015-0282(00)00518-5","article-title":"Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer","volume":"73","author":"Gardner","year":"2000","journal-title":"Fertil Steril"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1016\/j.rbmo.2011.06.004","article-title":"Spontaneous ovulation versus HCG triggering for timing natural-cycle frozen-thawed embryo transfer: a randomized study","volume":"23","author":"Weissman","year":"2011","journal-title":"Reprod Biomed Online"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1111\/j.1365-2605.2005.00630.x","article-title":"Sperm function tests and fertility","volume":"29","author":"Aitken","year":"2006","journal-title":"Int J Androl"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1007\/s10815-008-9250-1","article-title":"Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction","volume":"25","author":"Dirican","year":"2008","journal-title":"J Assist Reprod Genet"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/j.rbmo.2009.12.004","article-title":"Healthy baby born after reduction of sperm DNA fragmentation using cell sorting before ICSI","volume":"20","author":"Rawe","year":"2010","journal-title":"Reprod Biomed Online"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1002\/j.1939-4640.2003.tb02669.x","article-title":"Deterioration of plasma membrane is associated with active caspases in human spermatozoa","volume":"24","author":"Paasch","year":"2003","journal-title":"J Androl"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"e105","DOI":"10.1055\/s-0035-1548726","article-title":"First pregnancy, somatic and psychological status of a 4-year-old child born following annexin V TESA sperm separation","volume":"5","author":"Lukaszuk","year":"2015","journal-title":"AJP Rep"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1016\/S1472-6483(10)61118-2","article-title":"Advantage of combiningmagnetic cell separationwith sperm preparation techniques","volume":"10","author":"Said","year":"2005","journal-title":"Reprod Biomed Online"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1016\/j.fertnstert.2014.09.001","article-title":"Removal of annexin V-positive sperm cells for intracytoplasmic sperm injection in ovum donation cycles does not improve reproductive outcome: a controlled and randomized trial in unselected males","volume":"102","author":"Romany","year":"2014","journal-title":"Fertil Steril"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1111\/j.2047-2927.2013.00140.x","article-title":"Elimination of apoptotic spermatozoa by magnetic activated cell sorting improves the fertilization rate of couples treated with ICSI procedure","volume":"1","author":"Sheikhi","year":"2013","journal-title":"Andrology"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1080\/14647273.2018.1424354","article-title":"Clinical outcomes of magnetic activated sperm sorting in infertile men candidate for ICSI","volume":"22","author":"Ziarati","year":"2019","journal-title":"Hum Fertil (Camb)"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.rbmo.2013.06.014","article-title":"The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment","volume":"27","author":"Lewis","year":"2013","journal-title":"Reprod Biomed Online"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"14026","DOI":"10.3390\/ijms131114026","article-title":"Types, causes, detection and repair of DNA fragmentation in animal and human sperm cells","volume":"13","author":"Gonz\u00e1lez-Mar\u00edn","year":"2012","journal-title":"Int J Mol Sci"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1002\/j.1939-4640.2002.tb02599.x","article-title":"Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques","volume":"23","author":"Evenson","year":"2002","journal-title":"J Androl"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.fertnstert.2010.05.055","article-title":"Effect of sperm DNA fragmentation on pregnancy outcome depends on oocyte quality","volume":"95","author":"Meseguer","year":"2011","journal-title":"Fertil Steril"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1111\/andr.12049","article-title":"The negative influence of sperm cryopreservation on the quality and development of the embryo depends on the morphology of the oocyte","volume":"3","author":"Braga","year":"2015","journal-title":"Andrology"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1007\/s10815-013-0086-y","article-title":"Case report: the use of annexin V coupled with magnetic activated cell sorting in cryopreserved spermatozoa from a male cancer survivor: healthy twin newborns after two previous ICSI failures","volume":"30","author":"Herrero","year":"2013","journal-title":"J Assist Reprod Genet"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.rbmo.2014.10.018","article-title":"The effect of sperm DNA fragmentation on live birth rate after IVF or ICSI: a systematic review and meta-analysis","volume":"30","author":"Osman","year":"2015","journal-title":"Reprod Biomed Online"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.1007\/s10815-018-1242-1","article-title":"Magnetic-activated cell sorting of non-apoptotic spermatozoa improves the quality of embryos according to female age: a prospective sibling oocyte study","volume":"35","author":"Stimpfel","year":"2018","journal-title":"J Assist Reprod Genet"}],"container-title":["Asian Journal of Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/aja202161","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:09:41Z","timestamp":1787166581000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/aja202161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,22]]},"references-count":33,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022]]}},"URL":"https:\/\/doi.org\/10.4103\/aja202161","relation":{},"ISSN":["1008-682X","1745-7262"],"issn-type":[{"value":"1008-682X","type":"print"},{"value":"1745-7262","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,22]]},"assertion":[{"value":"2021-05-01","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-23","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}