{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T20:03:30Z","timestamp":1787169810517,"version":"build-2736575974"},"reference-count":44,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:p>\n                    To explore the relationship between genetic polymorphisms of metabolic enzymes such as\n                    <jats:italic toggle=\"yes\">CYP1A1<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">CYP2D6<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">GSTM1<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">GSTT1<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">GSTP1<\/jats:italic>\n                    and idiopathic male infertility. By observing the efficacy of antioxidants in the treatment of idiopathic male infertility, the effect of metabolic enzyme gene polymorphisms on antioxidant therapy in patients with idiopathic male infertility was prospectively studied. This case\u2013control study included 310 men with idiopathic infertility and 170 healthy controls. The cytochrome P450 1A1 (\n                    <jats:italic toggle=\"yes\">CYP1A1<\/jats:italic>\n                    ), cytochrome P450 2D6 (\n                    <jats:italic toggle=\"yes\">CYP2D6<\/jats:italic>\n                    ), glutathione S-transferase M1 (\n                    <jats:italic toggle=\"yes\">GSTM1<\/jats:italic>\n                    ), glutathione S-transferase T1 (\n                    <jats:italic toggle=\"yes\">GSTT1<\/jats:italic>\n                    ), and glutathione S-transferase P1 (\n                    <jats:italic toggle=\"yes\">GSTP1<\/jats:italic>\n                    ) genotypes in peripheral blood samples were analyzed by polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (PCR-RFLP). The idiopathic male infertility group was treated with vitamin C, vitamin E, and coenzyme Q10 for 3 months and followed up for 6 months.\n                    <jats:italic toggle=\"yes\">GSTM1<\/jats:italic>\n                    (\u2212),\n                    <jats:italic toggle=\"yes\">GSTT1<\/jats:italic>\n                    (\u2212), and\n                    <jats:italic toggle=\"yes\">GSTM1\/T1<\/jats:italic>\n                    (\u2212\/\u2212) in the idiopathic male infertility groups were more common than those in the control group. The sperm concentration, motility, viability, mitochondrial membrane potential (MMP), and seminal plasma total antioxidant capacity (T-AOC) level in patients with\n                    <jats:italic toggle=\"yes\">GSTM1<\/jats:italic>\n                    (\u2212),\n                    <jats:italic toggle=\"yes\">GSTT1<\/jats:italic>\n                    (\u2212), and\n                    <jats:italic toggle=\"yes\">GSTM1\/T1<\/jats:italic>\n                    (\u2212\/\u2212) were lower than those in wild-type carriers, and the sperm DNA fragmentation index (DFI), 8-hydroxy-2\u2019-deoxyguanosine (8-OH-dG), and malondialdehyde (MDA) and nitric oxide (NO) levels were higher. Therefore, oxidative damage may play an important role in the occurrence and development of idiopathic male infertility, but antioxidant therapy is not effective in male infertility patients with\n                    <jats:italic toggle=\"yes\">GSTM1<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">GSTT1<\/jats:italic>\n                    gene deletions.\n                  <\/jats:p>","DOI":"10.4103\/aja202180","type":"journal-article","created":{"date-parts":[[2021,11,14]],"date-time":"2021-11-14T03:09:04Z","timestamp":1636859344000},"page":"430-435","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":10,"title":["Metabolic enzyme gene polymorphisms predict the effects of antioxidant treatment on idiopathic male infertility"],"prefix":"10.4103","volume":"24","author":[{"given":"Hong-Yan","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Mu","sequence":"additional","affiliation":[{"name":"Department of Urology, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China"},{"name":"Department of Urology, The Sixth's Hospital of Guiyang of Guizhou Province, Guiyang 550005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Urology, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong-Dong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke-Hang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Urology, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Urology, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"He","sequence":"additional","affiliation":[{"name":"Department of Urology, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fa","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Urology, People's Hospital of Guizhou Province, Guiyang 550002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-Ping","family":"Xing","sequence":"additional","affiliation":[{"name":"Department of Urology, The First Affiliated Hospital of Medical College of Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai-Fa","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Urology, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China"},{"name":"Institute of Medical Science of Guizhou Medical University, Guiyang 550004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2021,11,16]]},"reference":[{"key":"-20240829","doi-asserted-by":"crossref","first-page":"1786","DOI":"10.1093\/humrep\/dex234","article-title":"The international glossary on infertility and fertility care, 2017","volume":"32","author":"Zegers-Hochschild","year":"2017","journal-title":"Hum Reprod"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s12958-015-0032-1","article-title":"A unique view on male infertility around the globe","volume":"13","author":"Agarwal","year":"2015","journal-title":"Reprod Biol Endocrin"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"296","DOI":"10.5534\/wjmh.190055","article-title":"Male oxidative stress infertility (MOSI): proposed terminology and clinical practice guidelines for management of idiopathic male infertility","volume":"37","author":"Agarwal","year":"2019","journal-title":"World J Mens Health"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1159\/000090873","article-title":"Clinical diagnosis in men undergoing infertility investigation in a University Hospital","volume":"76","author":"Pasqualotto","year":"2006","journal-title":"Urol Int"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"761264","DOI":"10.1155\/2014\/761264","article-title":"Oxidative stress, prooxidants, and antioxidants: the interplay","volume":"2014","author":"Rahal","year":"2014","journal-title":"Biomed Res Int"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/s40291-013-0028-5","article-title":"Applications of CYP450 testing in the clinical setting","volume":"17","author":"Samer","year":"2013","journal-title":"Mol Diagn Ther"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.pharmthera.2018.02.012","article-title":"Modulation of CYP1A1 metabolism: from adverse health effects to chemoprevention and therapeutic options","volume":"187","author":"Mescher","year":"2018","journal-title":"Pharmacol Ther"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1007\/s13318-015-0307-0","article-title":"Effect of 22 novel cytochrome P450 2D6 (CYP2D6) variants found in the Chinese population on hemangeol metabolism in vitro","volume":"41","author":"Liang","year":"2016","journal-title":"Eur J Drug Metab Pharmacokinet"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"257","DOI":"10.2164\/jandrol.110.012468","article-title":"Genetic polymorphisms of glutathione S-transferase M1, T1, and P1, and the assessment of oxidative damage in infertile men with varicoceles from northwestern China","volume":"33","author":"Tang","year":"2012","journal-title":"J Androl"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1016\/j.fertnstert.2008.07.017","article-title":"Association of CYP1A1 and glutathione S-transferase polymorphisms with male factor infertility","volume":"92","author":"Aydos","year":"2009","journal-title":"Fertil Steril"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1111\/j.1464-410X.2005.05328.x","article-title":"Oxidative stress, DNA damage and apoptosis in male infertility: a clinical approach","volume":"95","author":"Agarwal","year":"2005","journal-title":"BJU Int"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1093\/humupd\/dmp048","article-title":"World Health Organization reference values for human semen characteristics","volume":"16","author":"Cooper","year":"2010","journal-title":"Hum Reprod Update"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"1628","DOI":"10.1093\/humrep\/der132","article-title":"The role of sperm oxidative stress in male infertility and the significance of oral antioxidant therapy","volume":"26","author":"Gharagozloo","year":"2011","journal-title":"Hum Reprod"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"217","DOI":"10.3109\/14647273.2010.532279","article-title":"The role of antioxidant therapy in the treatment of male infertility","volume":"13","author":"Agarwal","year":"2010","journal-title":"Hum Fertil"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1093\/humrep\/del326","article-title":"Sperm DNA integrity assessment in prediction of assisted reproduction technology outcome","volume":"22","author":"Bungum","year":"2006","journal-title":"Hum Reprod"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1007\/s11626-016-0061-x","article-title":"Mitochondrial membrane potential (MMP) regulates sperm motility","volume":"52","author":"Agnihotri","year":"2016","journal-title":"In Vitro Cell Dev Biol Anim"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1093\/humrep\/dey038","article-title":"Accuracy of human sperm DNA oxidation quantification and threshold determination using an 8-OHdG immuno-detection assay","volume":"33","author":"Vorilhon","year":"2018","journal-title":"Hum Reprod"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"S235","DOI":"10.1016\/S0015-0282(03)01546-2","article-title":"Clinical diagnosis in men undergoing infertility investigation","volume":"80","author":"Pasqualotto","year":"2003","journal-title":"Fertil Steril"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/s00018-019-03253-8","article-title":"Oxidative stress and male infertility: current knowledge of pathophysiology and role of antioxidant therapy in disease management","volume":"77","author":"Barati","year":"2020","journal-title":"Cell Mol Life Sci"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1016\/j.rbmo.2014.02.004","article-title":"Sperm DNA damage caused by oxidative stress: modifiable clinical, lifestyle and nutritional factors in male infertility","volume":"28","author":"Wright","year":"2014","journal-title":"Reprod Biomed Online"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5534\/wjmh.2014.32.1.1","article-title":"Effect of oxidative stress on male reproduction","volume":"32","author":"Agarwal","year":"2014","journal-title":"World J Mens Health"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1038\/nrurol.2017.69","article-title":"Oxidative stress and male infertility","volume":"14","author":"Bisht","year":"2017","journal-title":"Nat Rev Urol"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1021\/tx700079z","article-title":"Cytochrome P450 and chemical toxicology","volume":"21","author":"Guengerich","year":"2008","journal-title":"Chem Res Toxicol"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/1479-7364-4-1-59","article-title":"The cytochrome P450 homepage","volume":"4","author":"Nelson","year":"2009","journal-title":"Hum Genomics"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1186\/1479-7364-1-6-460","article-title":"Analysis of the glutathione S-transferase (GST) gene family","volume":"1","author":"Nebert","year":"2004","journal-title":"Hum Genomics"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1159\/000028396","article-title":"Glutathione S-transferase polymorphisms and their biological consequences","volume":"61","author":"Hayes","year":"2000","journal-title":"Pharmacology"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1146\/annurev.pharmtox.45.120403.095857","article-title":"Glutathione transferases","volume":"45","author":"Hayes","year":"2004","journal-title":"Annu Rev Pharmacol"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.mrgentox.2009.09.011","article-title":"Genomic damages in peripheral blood lymphocytes and association with polymorphisms of three glutathione S-transferases in workers exposed to formaldehyde","volume":"695","author":"Jiang","year":"2010","journal-title":"Mutat Res"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1177\/1933719111413302","article-title":"Analysis of GSTM1, GSTT1, and CYP1A1 in idiopathic male infertility","volume":"19","author":"Salehi","year":"2011","journal-title":"Reprod Sci"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"e13703","DOI":"10.1111\/and.13703","article-title":"Oxidative stress markers in seminal plasma of idiopathic infertile men may be associated with glutathione S-transferase M1 and T1 null genotypes","volume":"52","author":"Barati","year":"2020","journal-title":"Andrologia"},{"key":"-20240829","article-title":"Review of clinical trials on effects of oral antioxidants on basic semen and other parameters in idiopathic oligoasthenoteratozoospermia","author":"Kumalic","year":"2014","journal-title":"Biomed Res Int 2014"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1093\/humrep\/dev302","article-title":"A novel antioxidant formulation designed to treat male infertility associated with oxidative stress: promising preclinical evidence from animal models","volume":"31","author":"Gharagozloo","year":"2016","journal-title":"Hum Reprod"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"1518","DOI":"10.1016\/j.fertnstert.2014.10.020","article-title":"Male infertility testing: reactive oxygen species and antioxidant capacity","volume":"102","author":"Ko","year":"2014","journal-title":"Fertil Steril"},{"key":"-20240829","first-page":"525","article-title":"Oxidative stress induced sperm DNA damage, a possible reason for male infertility","volume":"13","author":"Hosen","year":"2015","journal-title":"Iran J Reprod Med"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"143","DOI":"10.5534\/wjmh.2015.33.3.143","article-title":"Smoking and male infertility: an evidence-based review","volume":"33","author":"Harlev","year":"2015","journal-title":"World J Mens Health"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/j.eururo.2016.04.010","article-title":"Cigarette smoking and semen quality: a new meta-analysis examining the effect of the 2010 World Health Organization laboratory methods for the examination of human semen","volume":"70","author":"Sharma","year":"2016","journal-title":"Eur Urol"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"e13523","DOI":"10.1111\/and.13523","article-title":"Body mass index and age correlate with antioxidant supplementation effects on sperm quality: post hoc analyses from a double-blind placebo-controlled trial","volume":"52","author":"Busetto","year":"2020","journal-title":"Andrologia"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1016\/j.fertnstert.2010.10.005","article-title":"Vitamin E supplementation in semen-freezing medium improves the motility and protects sperm from freeze-thaw\u2013induced DNA damage","volume":"95","author":"Kalthur","year":"2011","journal-title":"Fertil Steril"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1111\/j.1479-828X.2007.00723.x","article-title":"A randomised control trial examining the effect of an antioxidant (Menevit) on pregnancy outcome during IVF-ICSI treatment","volume":"47","author":"Tremellen","year":"2007","journal-title":"Aust N Z J Obstet Gynaecol"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1093\/humrep\/14.4.1028","article-title":"Antioxidant treatment of patients with asthenozoospermia or moderate oligoasthenozoospermia with high-dose vitamin C and vitamin E: a randomized, placebo-controlled, double-blind study","volume":"14","author":"Rolf","year":"1999","journal-title":"Hum Reprod"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.freeradbiomed.2007.03.024","article-title":"Vitamin E, antioxidant and nothing more","volume":"43","author":"Traber","year":"2007","journal-title":"Free Radical Bio Med"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0098-2997(97)00036-8","article-title":"The effect of coenzyme Q10 on sperm motility and function","volume":"18","author":"Lewin","year":"1997","journal-title":"Mol Aspects Med"},{"key":"-20240829","doi-asserted-by":"crossref","first-page":"172","DOI":"10.3390\/biom8040172","article-title":"Effect of coenzyme Q10 supplementation on testosterone","volume":"8","author":"Banihani","year":"2018","journal-title":"Biomolecules"},{"key":"-20240829","first-page":"615","article-title":"[Tamoxifen combined with coenzyme Q10 for idiopathic oligoasthenospermia]","volume":"17","author":"Tang","year":"2011","journal-title":"Zhonghua Nan Ke Xue"}],"container-title":["Asian Journal of Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/aja202180","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:13:35Z","timestamp":1787166815000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/aja202180"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,16]]},"references-count":44,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022]]}},"URL":"https:\/\/doi.org\/10.4103\/aja202180","relation":{},"ISSN":["1008-682X","1745-7262"],"issn-type":[{"value":"1008-682X","type":"print"},{"value":"1745-7262","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,16]]},"assertion":[{"value":"2021-04-20","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-26","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}