{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:25:43Z","timestamp":1787761543959,"version":"build-2784847793"},"reference-count":31,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:p>\n                    We examined a cohort of 93 cystic fibrosis (CF) male patients who were pancreatic-sufficient (PS-CF; n=40) or pancreatic-insufficient (PI-CF;\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 53). Complex semen examination was performed, including standard semen analysis, quantitative karyological analysis (QKA) of immature germ cells (IGCs), transmission electronic microscopy (TEM), biochemical analysis, and sperm DNA fragmentation by terminal deoxynucleotidyl transferase-mediated dUTP nickend labeling (TUNEL) assay. Azoospermia was diagnosed in 83 (89.2%) patients. The other 10 (10.8%) patients were found to be nonazoospermic and showed various spermatological diagnoses (asthenozoospermia,\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 2; asthenoteratozoospermia,\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 3; oligoasthenozoospermia,\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 1; oligoasthenoteratozoospermia,\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 3; and normozoospermia,\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 1) with no specific morphological abnormalities. Oligospermia was detected in 89.2% azoospermic and 30.0% nonazoospermic patients. Low seminal pH (&lt;7.0) was found in 74 (89.2%) of 83 azoospermic patients. Moderate leukocytospermia (2.0 \u00d7 10\n                    <jats:sup>6<\/jats:sup>\n                    \u20132.2 \u00d7 10\n                    <jats:sup>6<\/jats:sup>\n                    ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) was revealed in 2.4% azoospermic and 40.0% nonazoospermic semen samples. The signs of partial meiotic arrest at prophase I were found in 4 of 6 nonazoospermic patients examined by QKA of IGCs. The content of fructose and citrate was low in oligospermic and normal in nonoligospermic semen samples. An increased percentage (&gt;30%) of spermatozoa with noncondensed (\u201cimmature\u201d) chromatin was revealed in 2 of 6 nonazoospermic semen samples analyzed by TEM.\n                  <\/jats:p>","DOI":"10.4103\/aja2022115","type":"journal-article","created":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T14:51:15Z","timestamp":1678373475000},"page":"591-597","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":4,"title":["Comprehensive semen examination in patients with pancreatic-sufficient and pancreatic-insufficient cystic fibrosis"],"prefix":"10.4103","volume":"25","author":[{"given":"Anna O","family":"Sedova","sequence":"first","affiliation":[{"name":"Research Centre for Medical Genetics, Moskvorechie Street 1, Moscow 115522, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria I","family":"Shtaut","sequence":"additional","affiliation":[{"name":"Research Centre for Medical Genetics, Moskvorechie Street 1, Moscow 115522, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizaveta E","family":"Bragina","sequence":"additional","affiliation":[{"name":"Research Centre for Medical Genetics, Moskvorechie Street 1, Moscow 115522, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatyana M","family":"Sorokina","sequence":"additional","affiliation":[{"name":"Research Centre for Medical Genetics, Moskvorechie Street 1, Moscow 115522, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galina V","family":"Shmarina","sequence":"additional","affiliation":[{"name":"Research Centre for Medical Genetics, Moskvorechie Street 1, Moscow 115522, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina V","family":"Andreeva","sequence":"additional","affiliation":[{"name":"Research Centre for Medical Genetics, Moskvorechie Street 1, Moscow 115522, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lyubov F","family":"Kurilo","sequence":"additional","affiliation":[{"name":"Research Centre for Medical Genetics, Moskvorechie Street 1, Moscow 115522, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanislav A","family":"Krasovskiy","sequence":"additional","affiliation":[{"name":"Research Centre for Medical Genetics, Moskvorechie Street 1, Moscow 115522, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksander V","family":"Polyakov","sequence":"additional","affiliation":[{"name":"Research Centre for Medical Genetics, Moskvorechie Street 1, Moscow 115522, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vyacheslav B","family":"Chernykh","sequence":"additional","affiliation":[{"name":"Research Centre for Medical Genetics, Moskvorechie Street 1, Moscow 115522, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2023,3,3]]},"reference":[{"key":"R1-20240829","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1126\/science.2475911","article-title":"Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA","volume":"245","author":"Riordan","year":"1989","journal-title":"Science"},{"key":"R2-20240829","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1093\/humrep\/der377","article-title":"CFTR mutations in men with congenital bilateral absence of the vas deferens (CBAVD): a systemic review and meta-analysis","volume":"27","author":"Yu","year":"2012","journal-title":"Hum Reprod"},{"key":"R3-20240829","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1016\/S1472-6483(10)60801-2","article-title":"Molecular pathology of the CFTR locus in male infertility","volume":"10","author":"Claustres","year":"2005","journal-title":"Reprod Biomed Online"},{"key":"R4-20240829","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1038\/ng.2745","article-title":"Defining the disease liability of variants in the cystic fibrosis transmembrane conductance regulator gene","volume":"45","author":"Sosnay","year":"2013","journal-title":"Nat Genet"},{"key":"R5-20240829","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1056\/NEJM197209212871204","article-title":"Fertility in males with cystic fibrosis","volume":"287","author":"Taussig","year":"1972","journal-title":"N Engl J Med"},{"key":"R6-20240829","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1093\/molehr\/2.9.669","article-title":"Mutations in the cystic fibrosis gene in men with congenital bilateral absence of the vas deferens","volume":"2","author":"De Braekeleer","year":"1996","journal-title":"Mol Hum Reprod"},{"key":"R7-20240829","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/s00018-016-2393-9","article-title":"Cystic fibrosis: a clinical view","volume":"74","author":"Castellani","year":"2016","journal-title":"Cell Mol Life Sci"},{"key":"R8-20240829","first-page":"906","article-title":"Differential cellular expression of cystic fibrosis transmembrane regulator in human reproductive tissues. Clues for the infertility in patients with cystic fibrosis","volume":"144","author":"Tizzano","year":"1994","journal-title":"Am J Pathol"},{"key":"R10-20240829","author":"Kurilo","journal-title":"Patent for Invention \u201cMethod of Cytological Diagnosis of Spermatogenesis Disorders\u201d, No. 2328736"},{"key":"R11-20240829","first-page":"33","article-title":"[Spermatogenesis evaluation using immature sex cells of the ejaculate","volume":"3","author":"Kurilo","year":"1995","journal-title":"Rus J Hum Reprod"},{"key":"R12-20240829","doi-asserted-by":"crossref","first-page":"305","DOI":"10.3109\/01485018008987000","article-title":"Morphological characterization of abnormal human spermatozoa using transmission electron microscopy","volume":"5","author":"Bartoov","year":"1980","journal-title":"Arch Androl"},{"key":"R13-20240829","doi-asserted-by":"crossref","first-page":"e13738","DOI":"10.1111\/and.13738","article-title":"TUNEL assay\u2013standardized method for testing sperm DNA fragmentation","volume":"53","author":"Sharma","year":"2021","journal-title":"Andrologia"},{"key":"R14-20240829","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1111\/j.1365-2605.1990.tb01040.x","article-title":"The influence of inflammation of the human male genital tract on secretion of the seminal markers alpha-glucosidase, glycerophosphocholine, carnitine, fructose and citric acid","volume":"13","author":"Cooper","year":"1990","journal-title":"Int J Androl"},{"key":"R15-20240829","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1056\/NEJM196807112790203","article-title":"Reproductive failure in males with cystic fibrosis","volume":"279","author":"Kaplan","year":"1968","journal-title":"N Engl J Med"},{"key":"R16-20240829","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1542\/peds.48.3.442","article-title":"Increased incidence of inguinal hernia, hydrocele, and undescended testicle in males with cystic fibrosis","volume":"48","author":"Holsclaw","year":"1971","journal-title":"Pediatrics"},{"key":"R17-20240829","doi-asserted-by":"crossref","first-page":"1401","DOI":"10.1016\/S0140-6736(82)92512-0","article-title":"Obstructive azoospermia as a diagnostic criterion for the cystic fibrosis syndrome","volume":"1","author":"Stern","year":"1982","journal-title":"Lancet"},{"key":"R18-20240829","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1016\/0140-6736(90)92066-Q","article-title":"Abnormal distribution of CF delta F508 allele in azoospermic men with congenital aplasia of epididymis and vas deferens","volume":"336","author":"Dumur","year":"1990","journal-title":"Lancet"},{"key":"R19-20240829","doi-asserted-by":"crossref","first-page":"1794","DOI":"10.1001\/jama.1992.03480130110034","article-title":"Congenital bilateral absence of the vas deferens. A primarily genital form of cystic fibrosis","volume":"267","author":"Anguiano","year":"1992","journal-title":"JAMA"},{"key":"R20-20240829","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1001\/jama.1996.03540080029022","article-title":"Diversity of reproductive tract abnormalities in men with cystic fibrosis","volume":"276","author":"Wilschanski","year":"1996","journal-title":"JAMA"},{"key":"R21-20240829","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1002\/1098-1004(200008)16:2<143::AID-HUMU7>3.0.CO;2-J","article-title":"Spectrum of CFTR mutations in cystic fibrosis and in congenital absence of the vas deferens in France","volume":"16","author":"Claustres","year":"2000","journal-title":"Hum Mutat"},{"key":"R22-20240829","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1136\/jmg.28.6.420","article-title":"A fertile male with cystic fibrosis: molecular genetic analysis","volume":"28","author":"Barreto","year":"1991","journal-title":"J Med Genet"},{"key":"R23-20240829","doi-asserted-by":"crossref","first-page":"e19120","DOI":"10.1371\/journal.pone.0019120","article-title":"Defective CFTR-dependent CREB activation results in impaired spermatogenesis and azoospermia","volume":"6","author":"Xu","year":"2011","journal-title":"PLoS One"},{"key":"R24-20240829","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.gyobfe.2015.03.009","article-title":"Congenital bilateral absence of vas deferens: from diagnosis to assisted reproductive techniques\u2013the experience of three centers","volume":"43","author":"Beauvillard","year":"2015","journal-title":"Gynecol Obstet Fertil"},{"key":"R25-20240829","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1097\/MCO.0b013e32832595b7","article-title":"Lipid metabolism in cystic fibrosis","volume":"12","author":"Worgall","year":"2009","journal-title":"Curr Opin Clin Nutr Metab Care"},{"key":"R26-20240829","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1111\/andr.12024","article-title":"Dietary fatty acids affect semen quality: a review","volume":"3","author":"Esmaeili","year":"2015","journal-title":"Andrology"},{"key":"R27-20240829","doi-asserted-by":"crossref","first-page":"9816","DOI":"10.1073\/pnas.0609253104","article-title":"Cystic fibrosis transmembrane conductance regulator is vital to sperm fertilizing capacity and male fertility","volume":"104","author":"Xu","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R28-20240829","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1038\/ng0293-157","article-title":"CFTR expression is regulated during both the cycle of the seminiferous epithelium and the oestrous cycle of rodents","volume":"3","author":"Trezise","year":"1993","journal-title":"Nat Genet"},{"key":"R29-20240829","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1093\/hmg\/2.3.213","article-title":"Expression of the cystic fibrosis gene in human foetal tissues","volume":"2","author":"Trezise","year":"1993","journal-title":"Hum Mol Genet"},{"key":"R30-20240829","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1111\/j.1365-2605.1991.tb01272.x","article-title":"The fertility potential of male cystic fibrosis patients","volume":"14","author":"Gottlieb","year":"1991","journal-title":"Int J Androl"},{"key":"R31-20240829","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1093\/humrep\/15.7.1613","article-title":"Histological and genetic analysis and risk assessment for chromosomal aberration after ICSI for patients presenting with CBAVD","volume":"15","author":"Viville","year":"2000","journal-title":"Hum Reprod"},{"key":"R32-20240829","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/S1472-6483(10)60922-4","article-title":"Chromatin condensation and DNA damage of human epididymal spermatozoa in obstructive azoospermia","volume":"8","author":"Ramos","year":"2004","journal-title":"Reprod Biomed Online"}],"container-title":["Asian Journal of Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/aja2022115","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:01:18Z","timestamp":1787760078000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/aja2022115"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,3]]},"references-count":31,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023]]}},"URL":"https:\/\/doi.org\/10.4103\/aja2022115","relation":{},"ISSN":["1745-7262","1008-682X"],"issn-type":[{"value":"1745-7262","type":"print"},{"value":"1008-682X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,3]]},"assertion":[{"value":"2022-05-21","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-15","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}