{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T08:01:02Z","timestamp":1791360062297,"version":"4.3.1"},"reference-count":40,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:p>\n                    Male germ cells employ specific metabolic pathways throughout their developmental stages. In a previous study, we discovered heightened expression of pyruvate kinase M (PKM), a pivotal glycolytic enzyme, in spermatogonia and spermatids. To gain deeper insights into PKM\u2019s roles in spermatogenesis, sperm function, and male fertility, we engineered a conditional-knockout mouse model (\n                    <jats:italic toggle=\"yes\">Pkm<\/jats:italic>\n                    -vKO mice) to selectively disrupt the\n                    <jats:italic toggle=\"yes\">Pkm<\/jats:italic>\n                    gene within germ cells. Despite maintaining regular testicular histology and sperm morphology, the male\n                    <jats:italic toggle=\"yes\">Pkm<\/jats:italic>\n                    -vKO mice were infertility, characterized by significant impairments in sperm motility and adenosine triphosphate (ATP) generation. In addition,\n                    <jats:italic toggle=\"yes\">Pkm<\/jats:italic>\n                    -null spermatozoa exhibited similar deficits in protein tyrosine phosphorylation linked to capacitation, as well as compromised performance in\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    fertilization experiments. To conclude, PKM\u2019s presence is not obligatory for the entirety of spermatogenesis in male germ cells; however, it emerges as a critical factor influencing sperm motility and overall male fertility.\n                  <\/jats:p>","DOI":"10.4103\/aja202350","type":"journal-article","created":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T01:00:05Z","timestamp":1698195605000},"page":"212-219","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":10,"title":["Pyruvate kinase M in germ cells is essential for sperm motility and male fertility but not spermatogenesis"],"prefix":"10.4103","volume":"26","author":[{"given":"Gao-Qing","family":"Qian","sequence":"first","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing 211166, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Chen","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Reproductive Medicine, Department of Histoembryology, Genetics and Developmental Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Shen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing 211166, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Liu","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Reproductive Medicine, Department of Histoembryology, Genetics and Developmental Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2023,10,24]]},"reference":[{"key":"R1-20240829","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1152\/physrev.00013.2015","article-title":"Spermatogenesis:the commitment to meiosis","volume":"96","author":"Griswold","year":"2016","journal-title":"Physiol Rev"},{"key":"R2-20240829","doi-asserted-by":"crossref","first-page":"5","DOI":"10.4103\/aja.aja_41_21","article-title":"Recent advances in isolation, identification, and culture of mammalian spermatogonial stem cells","volume":"24","author":"Xi","year":"2022","journal-title":"Asian J Androl"},{"key":"R3-20240829","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1038\/cr.2017.117","article-title":"Mettl3-\/Mettl14-mediated mRNA N(6)-methyladenosine modulates murine spermatogenesis","volume":"27","author":"Lin","year":"2017","journal-title":"Cell Res"},{"key":"R4-20240829","first-page":"817","article-title":"Ccdc87 is critical for sperm function and male fertility","volume":"99","author":"Wang","year":"2018","journal-title":"Biol Reprod"},{"key":"R5-20240829","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1093\/humupd\/dmw015","article-title":"The human epididymis:its function in sperm maturation","volume":"22","author":"Sullivan","year":"2016","journal-title":"Hum Reprod Update"},{"key":"R6-20240829","doi-asserted-by":"crossref","first-page":"R155","DOI":"10.1530\/REP-18-0589","article-title":"The impact of epididymal proteins on sperm function","volume":"158","author":"Bj\u00f6rkgren","year":"2019","journal-title":"Reproduction"},{"key":"R7-20240829","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1002\/mrd.22663","article-title":"Chang's meaning of capacitation:a molecular perspective","volume":"83","author":"Gervasi","year":"2016","journal-title":"Mol Reprod Dev"},{"key":"R8-20240829","doi-asserted-by":"crossref","first-page":"4471","DOI":"10.1242\/dev.090613","article-title":"The cell biology of mammalian fertilization","volume":"140","author":"Okabe","year":"2013","journal-title":"Development"},{"key":"R9-20240829","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.devcel.2020.01.014","article-title":"Metabolic changes accompanying spermatogonial stem cell differentiation","volume":"52","author":"Lord","year":"2020","journal-title":"Dev Cell"},{"key":"R10-20240829","doi-asserted-by":"crossref","first-page":"R55","DOI":"10.1530\/REP-21-0431","article-title":"Mitochondrial regulation in spermatogenesis","volume":"163","author":"Zhang","year":"2022","journal-title":"Reproduction"},{"key":"R11-20240829","doi-asserted-by":"crossref","first-page":"98","DOI":"10.3390\/antiox10010098","article-title":"Mitochondrial functionality in male fertility:from spermatogenesis to fertilization","volume":"10","author":"Park","year":"2021","journal-title":"Antioxidants (Basel)"},{"key":"R12-20240829","doi-asserted-by":"crossref","first-page":"e1569","DOI":"10.1002\/wsbm.1569","article-title":"Energy metabolism in mammalian sperm motility","volume":"14","author":"Amaral","year":"2022","journal-title":"WIREs Mech Dis"},{"key":"R13-20240829","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1093\/humupd\/dmi053","article-title":"Glycolysis and sperm motility:does a spoonful of sugar help the flagellum go round?","volume":"12","author":"Ford","year":"2006","journal-title":"Hum Reprod Update"},{"key":"R14-20240829","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1387\/ijdb.072522bs","article-title":"Mammalian sperm metabolism:oxygen and sugar, friend and foe","volume":"52","author":"Storey","year":"2008","journal-title":"Int J Dev Biol"},{"key":"R15-20240829","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1095\/biolreprod.103.026054","article-title":"Glycolysis plays a major role for adenosine triphosphate supplementation in mouse sperm flagellar movement","volume":"71","author":"Mukai","year":"2004","journal-title":"Biol Reprod"},{"key":"R16-20240829","doi-asserted-by":"crossref","first-page":"16501","DOI":"10.1073\/pnas.0407708101","article-title":"Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility","volume":"101","author":"Miki","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R17-20240829","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1038\/s41422-018-0074-y","article-title":"Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis","volume":"28","author":"Chen","year":"2018","journal-title":"Cell Res"},{"key":"R18-20240829","doi-asserted-by":"crossref","first-page":"1771","DOI":"10.1002\/pro.3691","article-title":"An overview of structure, function, and regulation of pyruvate kinases","volume":"28","author":"Schormann","year":"2019","journal-title":"Protein Sci"},{"key":"R19-20240829","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1002\/jcp.30536","article-title":"Multiple functions of pyruvate kinase M2 in various cell types","volume":"237","author":"Lee","year":"2022","journal-title":"J Cell Physiol"},{"key":"R20-20240829","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1007\/s13105-021-00813-0","article-title":"Posttranslational modification ofpyruvate kinase type M2 (PKM2):novel regulation of its biological roles to be further discovered","volume":"77","author":"Zheng","year":"2021","journal-title":"J Physiol Biochem"},{"key":"R21-20240829","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.freeradbiomed.2019.08.007","article-title":"Pyruvate kinase M2:a simple molecule with complex functions","volume":"143","author":"Alquraishi","year":"2019","journal-title":"Free Radic Biol Med"},{"key":"R22-20240829","first-page":"1655","article-title":"Pyruvate kinase M2 at a glance","volume":"128","author":"Yang","year":"2015","journal-title":"J Cell Sci"},{"key":"R23-20240829","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1002\/j.1939-4640.2001.tb02158.x","article-title":"Detection of the mouse acrosome reaction by acid phosphatase. Comparison with chlortetracycline and electron microscopy","volume":"22","author":"Pietrobon","year":"2001","journal-title":"J Androl"},{"key":"R24-20240829","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1242\/dev.121.4.1129","article-title":"Capacitation of mouse spermatozoa. I. Correlation between the capacitation state and protein tyrosine phosphorylation","volume":"121","author":"Visconti","year":"1995","journal-title":"Development"},{"key":"R25-20240829","first-page":"1502","article-title":"Retinoid signaling controls spermatogonial differentiation by regulating expression of replication-dependent core histone genes","volume":"143","author":"Chen","year":"2016","journal-title":"Development"},{"key":"R26-20240829","doi-asserted-by":"crossref","first-page":"1853","DOI":"10.2147\/IJN.S237254","article-title":"Acute damage to the sperm quality and spermatogenesis in male mice exposed to curcumin-loaded nanoparticles","volume":"15","author":"Xia","year":"2020","journal-title":"Int J Nanomedicine"},{"key":"R28-20240829","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/0012-1606(90)90254-G","article-title":"Structural and functional relationships between mouse and hamster zona pellucida glycoproteins","volume":"137","author":"Moller","year":"1990","journal-title":"Dev Biol"},{"key":"R29-20240829","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1093\/biolre\/iox103","article-title":"Sperm function, protein phosphorylation, and metabolism differ in mice lacking successive sperm-specific glycolytic enzymes","volume":"97","author":"Huang","year":"2017","journal-title":"Biol Reprod"},{"key":"R30-20240829","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1093\/biolre\/iox128","article-title":"Impaired sperm maturation in conditional Lcn6 knockout mice","volume":"98","author":"Yin","year":"2018","journal-title":"Biol Reprod"},{"key":"R31-20240829","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1002\/dvg.20310","article-title":"Generation of a germ cell-specific mouse transgenic Cre line, Vasa-Cre","volume":"45","author":"Gallardo","year":"2007","journal-title":"Genesis"},{"key":"R32-20240829","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1242\/dev.121.4.1139","article-title":"Capacitation of mouse spermatozoa. II. Protein tyrosine phosphorylation and capacitation are regulated by a cAMP-dependent pathway","volume":"121","author":"Visconti","year":"1995","journal-title":"Development"},{"key":"R33-20240829","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1002\/aja.1001590404","article-title":"Surface interaction in vitro between Sertoli cells and germ cells at different stages of spermatogenesis","volume":"159","author":"Ziparo","year":"1980","journal-title":"Am J Anat"},{"key":"R34-20240829","doi-asserted-by":"crossref","first-page":"3377","DOI":"10.1073\/pnas.80.11.3377","article-title":"Viability of rat spermatogenic cells in vitro is facilitated by their coculture with Sertoli cells in serum-free hormone-supplemented medium","volume":"80","author":"Tres","year":"1983","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R35-20240829","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1095\/biolreprod61.1.154","article-title":"Improvement of spermatogenesis in adult cryptorchid rat testis by intratesticular infusion of lactate","volume":"61","author":"Courtens","year":"1999","journal-title":"Biol Reprod"},{"key":"R36-20240829","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.tem.2004.07.003","article-title":"Lactate and energy metabolism in male germ cells","volume":"15","author":"Boussouar","year":"2004","journal-title":"Trends Endocrinol Metab"},{"key":"R37-20240829","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1038\/nrurol.2012.77","article-title":"Metabolic regulation is important for spermatogenesis","volume":"9","author":"Rato","year":"2012","journal-title":"Nat Rev Urol"},{"key":"R38-20240829","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1530\/jrf.0.0620399","article-title":"Exogenous lactate is essential for metabolic activities in isolated rat spermatocytes and spermatids","volume":"62","author":"Jutte","year":"1981","journal-title":"J Reprod Fertil"},{"key":"R39-20240829","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1095\/biolreprod.108.068353","article-title":"Expression of the gene for mouse lactate dehydrogenase C (Ldhc) is required for male fertility","volume":"79","author":"Odet","year":"2008","journal-title":"Biol Reprod"},{"key":"R40-20240829","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1095\/biolreprod.109.079699","article-title":"Phosphoglycerate kinase 2 (PGK2) is essential for sperm function and male fertility in mice","volume":"82","author":"Danshina","year":"2010","journal-title":"Biol Reprod"},{"key":"R41-20240829","doi-asserted-by":"crossref","first-page":"e21528","DOI":"10.1096\/fj.202100122R","article-title":"Starvation induces an increase in intracellular calcium and potentiates the progesterone-induced mouse sperm acrosome reaction","volume":"35","author":"S\u00e1nchez-C\u00e1rdenas","year":"2021","journal-title":"FASEB J"}],"container-title":["Asian Journal of Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/aja202350","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:47:26Z","timestamp":1787762846000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/aja202350"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,24]]},"references-count":40,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024]]}},"URL":"https:\/\/doi.org\/10.4103\/aja202350","relation":{},"ISSN":["1745-7262","1008-682X"],"issn-type":[{"value":"1745-7262","type":"print"},{"value":"1008-682X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,24]]},"assertion":[{"value":"2023-03-25","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-16","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}