{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T19:17:54Z","timestamp":1787771874670,"version":"build-2784847793"},"reference-count":24,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:p>\n                    Ex vivo tissue culture of the human corpus cavernosum (CC) can be used to explore the tissue structural changes and complex signaling networks. At present, artificial CC-like tissues based on acellular or three-dimensional (3D)-printed scaffolds are used to solve the scarcity of primary penis tissue samples. However, inconvenience and high costs limit the wide application of such methods. Here, we describe a simple, fast, and economical method of constructing artificial CC-like tissue. Human CC fibroblasts (FBs), endothelial cells (ECs), and smooth muscle cells (SMCs) were expanded\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    and mixed with Matrigel in specific proportions. A large number of bubbles were formed in the mixture by vortexing combined with pipette blowing, creating a porous, spongy, and spatial structure. The CC FBs produced a variety of signaling factors, showed multidirectional differentiation potential, and grew in a 3D grid in Matrigel, which is necessary for CC-like tissue to maintain a porous structure as a cell scaffold. Within the CC-like tissue, ECs covered the surface of the lumen, and SMCs were located inside the trabeculae, similar to the structure of the primary CC. Various cell components remained stable for 3 days\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    , but the EC content decreased on the 7\n                    <jats:sup>th<\/jats:sup>\n                    day. Wingless\/integrated (WNT) signaling activation led to lumen atrophy and increased tissue fibrosis in CC-like tissue, inducing the same changes in characteristics as in the primary CC. This study describes a preparation method for human artificial CC-like tissue that may provide an improved experimental platform for exploring the function and structure of the CC and conducting drug screening for erectile dysfunction therapy.\n                  <\/jats:p>","DOI":"10.4103\/aja202374","type":"journal-article","created":{"date-parts":[[2024,2,6]],"date-time":"2024-02-06T01:00:06Z","timestamp":1707181206000},"page":"260-267","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":1,"title":["Artificial cavernosa-like tissue based on multibubble Matrigel and a human corpus cavernous fibroblast scaffold"],"prefix":"10.4103","volume":"26","author":[{"given":"Yu-Zhuo","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Ultrasound, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000, China"},{"name":"Department of Interventional Medicine, Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Hong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Urology, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-Bo","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Urology, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Interventional Medicine, Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000, China"},{"name":"Department of Urology, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biao","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Interventional Medicine, Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000, China"},{"name":"Department of Urology, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying-Hao","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Interventional Medicine, Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000, China"},{"name":"Department of Urology, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Li","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Interventional Medicine, Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000, China"},{"name":"Department of Urology, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-Quan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Ultrasound, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Hong","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Ultrasound, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Ultrasound, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Li","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Ultrasound, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Ultrasound, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Ying","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Andrology, the Center for Men\u2019s Health, Urologic Medical Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi-Zhou","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Andrology, the Center for Men\u2019s Health, Urologic Medical Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Andrology, the Center for Men\u2019s Health, Urologic Medical Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Xing","sequence":"additional","affiliation":[{"name":"Cedars-Sinai Medical Center, Los Angeles, CA 90024, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Xin","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Urology, The Fifth Affiliated Hospital, Sun Yat-sen University, 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History"}},{"value":"2023-11-29","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}