{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:27:02Z","timestamp":1787840822540,"version":"build-2784847793"},"reference-count":93,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","license":[{"start":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T00:00:00Z","timestamp":1744675200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Lymphomas represent one of the most common malignant diseases in young men and an important issue is how treatments will affect their reproductive health. It has been hypothesized that chemotherapies, similarly to environmental chemicals, may alter the spermatogenic epigenome. Here, we report the genomic and epigenomic profiling of the sperm DNA from a 31-year-old Hodgkin lymphoma patient who faced recurrent spontaneous miscarriages in his couple 11\u201326 months after receiving chemotherapy with adriamycin, bleomycin, vinblastine, and dacarbazine (ABVD). In order to capture the potential deleterious impact of the ABVD treatment on mutational and methylation changes, we compared sperm DNA before and 26 months after chemotherapy with whole-genome sequencing (WGS) and reduced representation bisulfite sequencing (RRBS). The WGS analysis identified 403 variants following ABVD treatment, including 28 linked to genes crucial for embryogenesis. However, none were found in coding regions, indicating no impact of chemotherapy on protein function. The RRBS analysis identified 99 high-quality differentially methylated regions (hqDMRs) for which methylation status changed upon chemotherapy. Those hqDRMs were associated with 87 differentially methylated genes, among which 14 are known to be important or expressed during embryo development. While no variants were detected in coding regions, promoter regions of several genes potentially important for embryo development contained variants or displayed an altered methylated status. These might in turn modify the corresponding gene expression and thus affect their function during key stages of embryogenesis, leading to potential developmental disorders or miscarriages.<\/jats:p>","DOI":"10.4103\/aja2024107","type":"journal-article","created":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T11:05:03Z","timestamp":1744715103000},"page":"598-610","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":3,"title":["Recurrent spontaneous miscarriages from sperm after ABVD chemotherapy in a patient with Hodgkin\u2019s lymphoma: sperm DNA and methylation profiling"],"prefix":"10.4103","volume":"27","author":[{"given":"Gwendoline","family":"Lecuyer","sequence":"first","affiliation":[{"name":"Univ Rennes, Inserm, EHESP, Irset (Institut de Recherche en Sant\u00e9, Environnement et Travail) - UMR_S 1085, Rennes F-35000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoine D","family":"Rolland","sequence":"additional","affiliation":[{"name":"Univ Rennes, Inserm, EHESP, Irset (Institut de Recherche en Sant\u00e9, Environnement et Travail) - UMR_S 1085, Rennes F-35000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Sophie","family":"Neyroud","sequence":"additional","affiliation":[{"name":"Univ Rennes, Inserm, EHESP, Irset (Institut de Recherche en Sant\u00e9, Environnement et Travail) - UMR_S 1085, Rennes F-35000, France"},{"name":"CHU de Rennes, Departement de Gyn\u00e9cologie Obstetrique Reproduction-CECOS, 16 Boulevard de Bulgarie, Rennes F-35000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertrand","family":"Evrard","sequence":"additional","affiliation":[{"name":"Univ Rennes, Inserm, EHESP, Irset (Institut de Recherche en Sant\u00e9, Environnement et Travail) - UMR_S 1085, Rennes F-35000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan","family":"Alary","sequence":"additional","affiliation":[{"name":"Univ Rennes, Inserm, EHESP, Irset (Institut de Recherche en Sant\u00e9, Environnement et Travail) - UMR_S 1085, Rennes F-35000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clemence","family":"Genthon","sequence":"additional","affiliation":[{"name":"Institut National de Recherche pour l\u2019agriculture, l\u2019alimentation et l\u2019environnement (INRAE), Unit\u00e9 Service 1426 (US1426), Transcriptome Plateforme Technologique (GeT-PlaGe), Genotoul, Castanet-Tolosan 31326, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Dejucq-Rainsford","sequence":"additional","affiliation":[{"name":"Univ Rennes, Inserm, EHESP, Irset (Institut de Recherche en Sant\u00e9, Environnement et Travail) - UMR_S 1085, Rennes F-35000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9lia","family":"Ravel","sequence":"additional","affiliation":[{"name":"Univ Rennes, Inserm, EHESP, Irset (Institut de Recherche en Sant\u00e9, Environnement et Travail) - UMR_S 1085, Rennes F-35000, France"},{"name":"CHU de Rennes, Departement de Gyn\u00e9cologie Obstetrique Reproduction-CECOS, 16 Boulevard de Bulgarie, Rennes F-35000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessika","family":"Moreau","sequence":"additional","affiliation":[{"name":"ToxAlim (Research Center in Food Toxicology), Universit\u00e9 de Toulouse, INRA, ENVT, INP-Purpan, UPS, Toulouse F-31027, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Moinard","sequence":"additional","affiliation":[{"name":"Service de Biologie de la Reproduction et CECOS, H\u00f4pital Paule de Viguier, CHU Toulouse, 330 Avenue de Grande Bretagne, Toulouse 31059, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed Hadi Mohamed","family":"Abdelhamid","sequence":"additional","affiliation":[{"name":"ToxAlim (Research Center in Food Toxicology), Universit\u00e9 de Toulouse, INRA, ENVT, INP-Purpan, UPS, Toulouse F-31027, France"},{"name":"Department of Cell Biology and Tissue Culture, Biotechnology Research Center (BTRC), Ayn Zarah, Tripoli, Libya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Klopp","sequence":"additional","affiliation":[{"name":"ToxAlim (Research Center in Food Toxicology), Universit\u00e9 de Toulouse, INRA, ENVT, INP-Purpan, UPS, Toulouse F-31027, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis","family":"Bujan","sequence":"additional","affiliation":[{"name":"Service de Biologie de la Reproduction et CECOS, H\u00f4pital Paule de Viguier, CHU Toulouse, 330 Avenue de Grande Bretagne, Toulouse 31059, France"},{"name":"DEFE, Inserm1203 Toulouse III and Montpellier Universities, 330 Avenue de Grande Bretagne, Toulouse 31059, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Chalmel","sequence":"additional","affiliation":[{"name":"Univ Rennes, Inserm, EHESP, Irset (Institut de Recherche en Sant\u00e9, Environnement et Travail) - UMR_S 1085, Rennes F-35000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,4,15]]},"reference":[{"key":"R1-20250825","doi-asserted-by":"crossref","first-page":"E359","DOI":"10.1002\/ijc.29210","article-title":"Cancer incidence and mortality worldwide:sources, methods and major patterns in GLOBOCAN 2012","volume":"136","author":"Ferlay","year":"2015","journal-title":"Int J Cancer"},{"key":"R3-20250825","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1016\/S0140-6736(12)60605-9","article-title":"Non-Hodgkin 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