{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T22:25:49Z","timestamp":1793139949959,"version":"4.5.0"},"reference-count":55,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Recent evidence suggests that low-intensity extracorporeal shock wave therapy (Li-ESWT) is a promising treatment for chronic prostatitis\/chronic pelvic pain syndrome (CP\/CPPS); however, its safety in pelvic organs, particularly prostate tissues and cells, remains unclear. The current study evaluates the risks of prostate cell damage or oncogenesis following the administration of Li-ESWT for prostatitis. To this end, a robust\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    model (Cell Counting Kit-8 [CCK-8] assay, clone formation assay, cell scratch assay, lactate dehydrogenase [LDH] release assay, flow cytometry, and immunoblotting assay) was designed to examine the effects of Li-ESWT on cell proliferation, clonogenicity, migration, membrane integrity, and DNA damage. Exome sequencing of Li-ESWT-treated cells was performed to determine the risk of carcinogenesis. Furthermore, an in vivo rat model (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 20) was employed to assess the effects of Li-ESWT on cancer biomarkers (carcinoembryonic antigen [CEA], Ki67, proliferating cell nuclear antigen [PCNA], and gamma-H2A histone family member X, phosphorylation of the H2AX Ser-139 [\n                    <jats:italic toggle=\"yes\">\u03b3<\/jats:italic>\n                    -H2AX]) in prostate tissue. Based on our findings, Li-ESWT promotes cellular growth and motility without inducing significant cell membrane or DNA damage or alterations. Genetic analyses did not demonstrate an increase in mutations, and no damage to prostate tissue or upregulation of cancer biomarkers was detected in vivo. This comprehensive\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    assessment confirms the safety of Li-ESWT in managing prostate disorders.\n                  <\/jats:p>","DOI":"10.4103\/aja202448","type":"journal-article","created":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T01:00:23Z","timestamp":1722992423000},"page":"535-543","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":3,"title":["Safety of low-intensity extracorporeal shock wave therapy in prostate disorders: in vitro and in vivo evidence"],"prefix":"10.4103","volume":"26","author":[{"given":"Yi-Ran","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Urology, Gansu Province Clinical Research Center for Urinary System Disease, The Second Hospital and Clinical Medical School, Lanzhou University, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Urology, Gansu Provincial Hospital, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Bo","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Radiotherapy, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Wen","family":"Gong","sequence":"additional","affiliation":[{"name":"Institute of Urology, Gansu Province Clinical Research Center for Urinary System Disease, The Second Hospital and Clinical Medical School, Lanzhou University, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang-Bin","family":"Kong","sequence":"additional","affiliation":[{"name":"Institute of Urology, Gansu Province Clinical Research Center for Urinary System Disease, The Second Hospital and Clinical Medical School, Lanzhou University, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Urology, Gansu Provincial Second People\u2019s Hospital, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Long","family":"Dong","sequence":"additional","affiliation":[{"name":"Institute of Urology, Gansu Province Clinical Research Center for Urinary System Disease, The Second Hospital and Clinical Medical School, Lanzhou University, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-Qiang","family":"Tian","sequence":"additional","affiliation":[{"name":"Institute of Urology, Gansu Province Clinical Research Center for Urinary System Disease, The Second Hospital and Clinical Medical School, Lanzhou University, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Ping","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Urology, Gansu Province Clinical Research Center for Urinary System Disease, The Second Hospital and Clinical Medical School, Lanzhou University, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2024,8,6]]},"reference":[{"key":"R1-20240905","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1016\/S0140-6736(80)92335-1","article-title":"Extracorporeally induced destruction of kidney stones by shock waves","volume":"316","author":"Chaussy","year":"1980","journal-title":"Lancet"},{"key":"R2-20240905","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1007\/BF02703708","article-title":"Shock wave treatment in medicine","volume":"30","author":"Shrivastava","year":"2005","journal-title":"J 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