{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T09:13:24Z","timestamp":1788599604568,"version":"build-2803163510"},"reference-count":43,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","license":[{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:p>\n                    Human sperm cryopreservation is essential for assisted reproductive technology; however, the freeze\u2013thaw process reduces motility and increases DNA fragmentation. Rho-associated coiled-coil kinase (ROCK) inhibitors improve post-thaw survival in several cell types, yet their application to human sperm remains underexplored. We evaluated whether ROCK inhibition reduces DNA fragmentation during cryopreservation by liquid nitrogen vapor slow freezing or vitrification. Each semen sample was divided into four aliquots: 2 were cryopreserved via liquid nitrogen vapor slow freezing with or without ROCK inhibitor and 2 underwent vitrification with or without ROCK inhibitor. After 1 week of cryostorage, samples were thawed at the room temperature, and DNA fragmentation was quantified using the terminal deoxynucleotidyl transferase dUTP nick end labeling assay. DNA fragmentation decreased with ROCK inhibitor versus control after liquid nitrogen vapor slow freezing (mean \u00b1 standard deviation [s.d.]: 10.47% \u00b1 6.77%\n                    <jats:italic toggle=\"yes\">vs<\/jats:italic>\n                    13.54% \u00b1 7.0%;\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    = 0.002) and after vitrification (mean \u00b1 s.d.: 12.51% \u00b1 5.84%\n                    <jats:italic toggle=\"yes\">vs<\/jats:italic>\n                    16.5% \u00b1 10.77%;\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    = 0.006). ROCK inhibition thus reduces post-thaw sperm DNA fragmentation with both cryopreservation methods, particularly with liquid nitrogen vapor slow freezing, supporting its potential to improve sperm storage for assisted reproductive technology.\n                  <\/jats:p>","DOI":"10.4103\/aja2025107","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T07:05:41Z","timestamp":1777359941000},"page":"667-673","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Rho-kinase inhibition reduces DNA fragmentation in human sperm cryopreservation"],"prefix":"10.4103","volume":"28","author":[{"given":"Pawan","family":"Limvorapitux","sequence":"first","affiliation":[{"name":"Infertility Unit, Department of Obstetrics and Gynecology, Faculty of Medicine Vajira Hospital, Navamindradhiraj University, Bangkok 10300, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isarin","family":"Thanaboonyawat","sequence":"additional","affiliation":[{"name":"Reproductive and Biology Unit, Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Japarath","family":"Prechapanich","sequence":"additional","affiliation":[{"name":"Reproductive and Biology Unit, Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pitak","family":"Laokirkkiat","sequence":"additional","affiliation":[{"name":"Reproductive and Biology Unit, Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roungsin","family":"Choavaratana","sequence":"additional","affiliation":[{"name":"Reproductive and Biology Unit, Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somsin","family":"Petyim","sequence":"additional","affiliation":[{"name":"Reproductive and Biology Unit, Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"},{"name":"Center of Reproductive and Stem Cell Biology, Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, 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