{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T16:09:04Z","timestamp":1792598944916,"version":"4.5.0"},"reference-count":29,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","license":[{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Age-related erectile dysfunction (ARED) represents a significant clinical challenge due to the interplay between chronic comorbidities and age-related physiological decline. This study investigated the therapeutic potential of near-infrared photobiomodulation therapy (NIR-PBMT) in ARED mice, focusing on molecular and physiological mechanisms of complex erectile function restoration. Aged mice received NIR-PBMT (4 J cm\n                    <jats:sup>\u22122<\/jats:sup>\n                    ) every 48 h for 2 weeks. Erectile function was evaluated using the maximum intracavernosal pressure\/mean arterial pressure (ICP\n                    <jats:sub>max<\/jats:sub>\n                    \/MAP) ratio following cavernous nerve stimulation. Histological analysis and western blotting revealed significant improvements in penile tissue architecture, including increased smooth muscle content, reduced collagen deposition, and altered expression of senescence markers (p21 and phosphorylated H2A histone family member X [\n                    <jats:italic toggle=\"yes\">\u03b3<\/jats:italic>\n                    -H2A.X]) and endothelial nitric oxide synthase (eNOS).\n                    <jats:italic toggle=\"yes\">In vitro<\/jats:italic>\n                    studies using human corpus cavernous endothelial cells (HCCECs) demonstrated that NIR-PBMT reduced cellular senescence (assessed via SA-\n                    <jats:italic toggle=\"yes\">\u03b2<\/jats:italic>\n                    -galactosidase staining), enhanced nitric oxide (NO) production, and improved mitochondrial network integrity. Angiogenesis assays further confirmed the pro-angiogenic effects of NIR-PBMT. Collectively, these findings highlight NIR-PBMT as a promising non-invasive therapy for ARED, acting through multiple pathways to reverse pathological remodeling and restore endothelial function. Future translational research is necessary to validate its clinical efficacy and optimize treatment protocols.\n                  <\/jats:p>","DOI":"10.4103\/aja202532","type":"journal-article","created":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T10:00:12Z","timestamp":1753783212000},"page":"80-88","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":4,"title":["Near-infrared photobiomodulation therapy for age-related erectile dysfunction: molecular and physiological restoration in a mouse model"],"prefix":"10.4103","volume":"28","author":[{"given":"Zhong-Jie","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Urology, Peking University Third Hospital, Peking University, Beijing 100191, China"},{"name":"Reproductive Medicine Center, Peking University Third Hospital, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Urology, Peking University Third Hospital, Peking University, Beijing 100191, China"},{"name":"Reproductive Medicine Center, Peking University Third Hospital, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian-Xi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Urology, Peking University Third Hospital, Peking University, Beijing 100191, China"},{"name":"Reproductive Medicine Center, Peking University Third Hospital, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Hao","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Urology, Peking University Third Hospital, Peking University, Beijing 100191, China"},{"name":"Reproductive Medicine Center, Peking University Third Hospital, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Urology, Princess Alexandra Hospital, University of Queensland, Brisbane, QLD 4000, Australia"},{"name":"AndroUrology Centre, Brisbane, QLD 4000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Urology, Peking University Third Hospital, Peking University, Beijing 100191, China"},{"name":"Reproductive Medicine Center, Peking University Third Hospital, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-Dong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology, Peking University Third Hospital, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao-Cheng","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Urology, Peking University Third Hospital, Peking University, Beijing 100191, China"},{"name":"Reproductive Medicine Center, Peking University Third Hospital, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,7,29]]},"reference":[{"key":"R1-20260122","doi-asserted-by":"crossref","first-page":"16003","DOI":"10.1038\/nrdp.2016.3","article-title":"Erectile dysfunction","volume":"2","author":"Yafi","year":"2016","journal-title":"Nat Rev Dis Primers"},{"key":"R2-20260122","doi-asserted-by":"crossref","first-page":"100380","DOI":"10.1016\/j.jacadv.2023.100380","article-title":"Elevated cardiac biomarkers, erectile dysfunction, and mortality in U. S. men:NHANES 2001 to 2004","volume":"2","author":"Selvin","year":"2023","journal-title":"JACC Adv"},{"key":"R3-20260122","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1016\/j.eururo.2013.08.023","article-title":"A systematic review of the association between erectile dysfunction and cardiovascular disease","volume":"65","author":"Gandaglia","year":"2014","journal-title":"Eur Urol"},{"key":"R4-20260122","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1007\/s11357-021-00507-x","article-title":"Early manifestation of aging-related vascular dysfunction in human penile vasculature-a potential explanation for the role of erectile dysfunction as a harbinger of systemic vascular disease","volume":"44","author":"Assar","year":"2022","journal-title":"Geroscience"},{"key":"R5-20260122","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.sxmr.2015.10.011","article-title":"Aging and erectile dysfunction","volume":"4","author":"Echeverri Tirado","year":"2016","journal-title":"Sex Med Rev"},{"key":"R6-20260122","doi-asserted-by":"crossref","first-page":"20","DOI":"10.21037\/tau.2016.11.18","article-title":"Aging related erectile dysfunction-potential mechanism to halt or delay its onset","volume":"6","author":"Ferrini","year":"2017","journal-title":"Transl Androl Urol"},{"key":"R7-20260122","doi-asserted-by":"crossref","first-page":"112232","DOI":"10.1016\/j.exger.2023.112232","article-title":"Mechanism associated with changes in male reproductive functions during ageing process","volume":"179","author":"Ajayi","year":"2023","journal-title":"Exp Gerontol"},{"key":"R8-20260122","first-page":"CD013071","article-title":"Testosterone replacement in men with sexual dysfunction","volume":"1","author":"Lee","year":"2024","journal-title":"Cochrane Database Syst Rev"},{"key":"R9-20260122","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.eururo.2007.02.034","article-title":"Testosterone and erectile function:from basic research to a new clinical paradigm for managing men with androgen insufficiency and erectile dysfunction","volume":"52","author":"Traish","year":"2007","journal-title":"Eur Urol"},{"key":"R10-20260122","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1016\/j.ucl.2021.06.013","article-title":"Energy-based therapies for erectile dysfunction:current and future directions","volume":"48","author":"Pai","year":"2021","journal-title":"Urol Clin North Am"},{"key":"R11-20260122","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.jsxm.2020.10.021","article-title":"Endovascular therapy for arteriogenic erectile dysfunction with a novel sirolimus-eluting stent","volume":"18","author":"Sch\u00f6nhofen","year":"2021","journal-title":"J Sex Med"},{"key":"R12-20260122","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1093\/sxmrev\/qead043","article-title":"Hyperbaric oxygen therapy as a treatment for erectile dysfunction:a meta-analysis","volume":"12","author":"Saffati","year":"2023","journal-title":"Sex Med Rev"},{"key":"R13-20260122","doi-asserted-by":"crossref","first-page":"1724","DOI":"10.3390\/jcm9061724","article-title":"Photobiomodulation-underlying mechanism and clinical applications","volume":"9","author":"Dompe","year":"2020","journal-title":"J Clin Med"},{"key":"R14-20260122","doi-asserted-by":"crossref","first-page":"101415","DOI":"10.1016\/j.arr.2021.101415","article-title":"Photobiomodulation for the aging brain","volume":"70","author":"Cardoso","year":"2021","journal-title":"Ageing Res Rev"},{"key":"R15-20260122","doi-asserted-by":"crossref","first-page":"282","DOI":"10.23736\/S1973-9087.21.07236-1","article-title":"Low-intensity LASER and LED (photobiomodulation therapy) for pain control of the most common musculoskeletal conditions","volume":"58","author":"DE Oliveira","year":"2022","journal-title":"Eur J Phys Rehabil Med"},{"key":"R16-20260122","doi-asserted-by":"crossref","first-page":"418","DOI":"10.3390\/biom13030418","article-title":"Photobiomodulation for chemotherapy-induced oral mucositis in pediatric patients","volume":"13","author":"Hafner","year":"2023","journal-title":"Biomolecules"},{"key":"R17-20260122","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1097\/ICU.0000000000000742","article-title":"Photobiomodulation therapy in age-related macular degeneration","volume":"32","author":"Muste","year":"2021","journal-title":"Curr Opin Ophthalmol"},{"key":"R18-20260122","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.niox.2022.11.005","article-title":"Photobiomodulation and nitric oxide signaling","volume":"130","author":"Kashiwagi","year":"2023","journal-title":"Nitric Oxide"},{"key":"R19-20260122","doi-asserted-by":"crossref","first-page":"1472","DOI":"10.1111\/andr.13422","article-title":"Effect of near-infrared laser treatment on improving erectile function in rats with diabetes mellitus","volume":"11","author":"Yang","year":"2023","journal-title":"Andrology"},{"key":"R20-20260122","doi-asserted-by":"crossref","first-page":"197","DOI":"10.5534\/wjmh.230362","article-title":"The effects of a red-light controllable nitric oxide donor, NORD-1, on erectile dysfunction in rats with streptozotocin induced diabetes mellitus","volume":"43","author":"Hotta","year":"2025","journal-title":"World J Mens Health"},{"key":"R21-20260122","doi-asserted-by":"crossref","first-page":"842","DOI":"10.5534\/wjmh.230187","article-title":"Photobiomodulation as a potential therapy for erectile function:a preclinical study in a cavernous nerve injury model","volume":"42","author":"Anita","year":"2024","journal-title":"World J Mens Health"},{"key":"R22-20260122","doi-asserted-by":"crossref","first-page":"RP88942","DOI":"10.7554\/eLife.88942","article-title":"Single-cell transcriptome analysis of cavernous tissues reveals the key roles of pericytes in diabetic erectile dysfunction","volume":"12","author":"Bae","year":"2024","journal-title":"Elife"},{"key":"R23-20260122","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji:an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat Methods"},{"key":"R24-20260122","doi-asserted-by":"crossref","first-page":"106343","DOI":"10.1016\/j.biocel.2022.106343","article-title":"Heterogeneity of fibroblasts is a hallmark of age-associated erectile dysfunction","volume":"156","author":"Liu","year":"2023","journal-title":"Int J Biochem Cell Biol"},{"key":"R25-20260122","doi-asserted-by":"crossref","first-page":"4302","DOI":"10.1038\/s41467-022-31950-9","article-title":"Single-cell transcriptome atlas of the human corpus cavernosum","volume":"13","author":"Zhao","year":"2022","journal-title":"Nat Commun"},{"key":"R26-20260122","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1038\/ijir.2011.2","article-title":"eNOS-uncoupling in age-related erectile dysfunction","volume":"23","author":"Johnson","year":"2011","journal-title":"Int J Impot Res"},{"key":"R27-20260122","doi-asserted-by":"crossref","first-page":"197","DOI":"10.21037\/tau-22-511","article-title":"Study on the mechanism of aging-related erectile dysfunction based on bioinformatics and experimental verification","volume":"12","author":"Sun","year":"2023","journal-title":"Transl Androl Urol"},{"key":"R28-20260122","doi-asserted-by":"crossref","first-page":"e0249487","DOI":"10.1371\/journal.pone.0249487","article-title":"D-(+)-Galactose-induced aging:a novel experimental model of erectile dysfunction","volume":"16","author":"de Almeida Rezende","year":"2021","journal-title":"PLoS One"},{"key":"R29-20260122","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1038\/s41580-024-00738-8","article-title":"SenNet recommendations for detecting senescent cells in different tissues","volume":"25","author":"Suryadevara","year":"2024","journal-title":"Nat Rev Mol Cell Biol"}],"container-title":["Asian Journal of Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/aja202532","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:18:23Z","timestamp":1787761103000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/aja202532"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,29]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.4103\/aja202532","relation":{},"ISSN":["1745-7262","1008-682X"],"issn-type":[{"value":"1745-7262","type":"print"},{"value":"1008-682X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,29]]},"assertion":[{"value":"2024-08-09","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-08","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}