{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T16:42:26Z","timestamp":1786725746174,"version":"build-2736575974"},"reference-count":37,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:00:00Z","timestamp":1778544000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:p>\n                    This study identified family with sequence similarity 205, member A (FAM205A) as a clinically significant acrosomal protein critically implicated in male idiopathic infertility. Integrated proteomic analysis of sperm from in vitro fertilization (IVF) patients categorized by fertilization outcome (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 12 per group; clinical validation,\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 267) revealed a strong correlation between FAM205A expression and fertilization success (76.3% in high-expression groups vs 15.4% in low-expression groups,\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    &lt; 0.05), independent of standard semen parameters. Functional analyses utilizing pooled human sperm samples (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 20 per group) revealed that low FAM205A expression led to a significantly decreased calcium-induced acrosome reaction response (28.9% vs 49.2% in high FAM205A expression,\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    &lt; 0.01), a phenotype corroborated by anti-FAM205A antibody blockade (38.1% inhibition,\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    &lt; 0.01). The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein 9 (Cas9) genome editing system (CRISPR-Cas9)-generated knockout mice lacking\n                    <jats:italic toggle=\"yes\">Fam205a1<\/jats:italic>\n                    , which is the murine ortholog of human FAM205A, exhibited complete infertility, characterized by oligospermia, asthenozoospermia, teratozoospermia, disrupted acrosome formation, and significantly reduced sperm motility. Mechanistic studies in Fam205a1 knockout mice revealed that FAM205A deficiency induces spermatid apoptosis and impairs acrosomal exocytosis, a crucial process for sperm\u2013egg fusion. The results demonstrate that FAM205A regulates fertility through two mechanisms: preserving structural integrity during spermiogenesis and facilitating the acrosomal reaction (AR). This study identified FAM205A as a reliable diagnostic biomarker for idiopathic male infertility and a potential treatment target in reproductive medicine.\n                  <\/jats:p>","DOI":"10.4103\/aja202580","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T06:00:25Z","timestamp":1778565625000},"page":"454-466","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Comparative sperm proteomics in IVF patients with differential fertilization rates identifies FAM205A as a novel biomarker for male infertility"],"prefix":"10.4103","volume":"28","author":[{"given":"Peng","family":"Zhang","sequence":"first","affiliation":[{"name":"Reproductive Medicine Center, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China"},{"name":"Department of Obstetrics and Gynecology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China"},{"name":"Key Laboratory for Molecular Diagnosis of Hubei Province, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He-Mei","family":"Li","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China"},{"name":"Department of Obstetrics and Gynecology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sai","family":"Lu","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China"},{"name":"Department of Obstetrics and Gynecology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Qing","family":"Fan","sequence":"additional","affiliation":[{"name":"Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha 410008, China"},{"name":"The Reproduction Center of Reproductive and Genetic Hospital, CITIC-XIANGYA, Changsha 410008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Qian","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China"},{"name":"Department of Obstetrics and Gynecology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiu","family":"Yin","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China"},{"name":"Department of Obstetrics and Gynecology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2026,5,12]]},"reference":[{"key":"R1-20260702","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1038\/s41585-018-0003-3","article-title":"Genetics of male infertility","volume":"15","author":"Krausz","year":"2018","journal-title":"Nat Rev Urol"},{"key":"R2-20260702","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1093\/humupd\/dmp048","article-title":"World Health Organization reference values for human 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