{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T05:40:34Z","timestamp":1792561234033,"version":"4.5.0"},"reference-count":43,"publisher":"Ovid Technologies (Wolters Kluwer Health)","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"abstract":"<jats:p>\n                    Rising global temperatures have intensified male infertility linked to testicular heat stress. While microRNAs play pivotal roles in spermatogenesis, the reason for aberrant microRNA expression following heat stress-induced spermatogenic disorders remains largely unclear. We established a murine heat stress model and performed small RNA sequencing. Candidate microRNAs were obtained via intersection with additional spermatogenic arrest and Sertoli cell-only syndrome datasets. The proliferation of germ cells was assessed by cholecystokinin-8 and 5-ethynyl-2\u2019-deoxyuridine assays. Bioinformatic analysis was applied to predict downstream miRNA targets, and enrichment analysis was performed. Fluorescence\n                    <jats:italic toggle=\"yes\">in situ<\/jats:italic>\n                    hybridization and immunofluorescence assays were utilized to determine the expression of miRNAs and their targets in Sertoli cell-only syndrome models and clinical testicular samples. In total, testicular heat stress resulted in 102 upregulated and 114 downregulated miRNAs. Four candidate microRNAs (miR-378a-3p, miR-29c-3p, let-7a-5p, and miR-9-5p) were screened for further investigation. Functional assays demonstrated that elevated expression of miR-378a-3p, miR-29c-3p, or silencing of miR-9-5p, notably inhibited the proliferation ability of spermatogonia (GC-1 cells). Common targets of these microRNAs were highly enriched in collagen-related pathways, predominantly through the inhibition of miR-29c-3p-targeted collagen type I alpha 1 and type III alpha 1 chain 1. Compared with normal tissue samples, tissue samples from Sertoli cell-only syndrome model mice and from those with spermatogenic disorders also exhibited high miR-29c-3p expression levels. In summary, our study identified microRNA expression patterns during testicular heat stress and preliminarily investigated the roles of specific microRNAs. miR-29c-3p and the collagen synthesis pathway could be utilized as biomarkers and therapeutic targets for heat-induced spermatogenic disorders.\n                  <\/jats:p>","DOI":"10.4103\/aja202623","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T05:00:17Z","timestamp":1792558817000},"update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Dysregulation of the miR-29c-3p\/collagen axis induces spermatogenic failure in heat stress and non-obstructive azoospermia"],"prefix":"10.4103","author":[{"given":"Shou-Bo","family":"Zhang","sequence":"first","affiliation":[{"name":"Center for Reproductive Medicine, Guangdong Armed Police Hospital, Guangzhou 510000, 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