{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:39:09Z","timestamp":1786649949983,"version":"build-2736575974"},"reference-count":16,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T00:00:00Z","timestamp":1683763200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>INTRODUCTION:<\/jats:title>\n                    <jats:p>Platelet transfusion has been therapeutically used in patients with thrombocytopenia and platelet function defects over the years. The use of advanced techniques may add value in assessing the quality of platelet products. The aim of the study was to assess stored platelet concentrates (PCs) prepared in blood banks for platelet indices, clot strength, and platelet function.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>MATERIALS AND METHODS:<\/jats:title>\n                    <jats:p>Apart from the routine quality check parameters (platelet count, volume, pH, swirling, and sterility), 24-h-old PCs derived by the platelet-rich plasma method (PRP-PC) were prospectively assessed for various platelet indices using hematology analyzer and clot strength using thromboelastography (TEG). Platelet function (platelet activation and aggregation) was assessed using flow cytometry and compared with freshly prepared PRP.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS:<\/jats:title>\n                    <jats:p>\n                      A total of 43 PRP-PCs that were analyzed had a mean volume of 69.1 \u00b1 5.1 mL, a pH of 6.8 \u00b1 0.3, and a platelet count of 738.8 \u00b1 312.9 \u00d7 10\n                      <jats:sup>3<\/jats:sup>\n                      cells\/\u03bcL. Swirling was present in all. The platelet distribution width, mean platelet volume, platelet-large cell ratio, plateletcrit, and immature platelet fraction were 9.4 \u00b1 2.7 fL, 8.7 \u00b1 1.2 fL, 16.9% \u00b1 8.9%, 0.6% \u00b1 0.3%, and 4.4% \u00b1 3.9%, respectively. The r-time, k-time, alpha angle, and maximum amplitude were 15 \u00b1 0.3 min, 2.1 \u00b1 0.4 min, 65.2\u00b0 \u00b1 2.3\u00b0, and 76.1 \u00b1 10.1 mm, respectively. Delayed clot initiation and higher clot strength were observed in in 60% (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 21) and 71.4% (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 25) of products, respectively Platelet activation of PRP-PC was significantly lower than PRP (0.4 [0.1%\u201342%] vs. 10.5 [0.8%\u201332.4%];\n                      <jats:italic toggle=\"yes\">P =<\/jats:italic>\n                      0.002). However, platelet aggregation of PRP-PC was significantly higher than PRP (62.0 [26.7%\u201388.7%] vs. 33.4 [4.9%\u201355.8%];\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.003).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION:<\/jats:title>\n                    <jats:p>Platelet indices, TEG, and flow cytometry analysis provides useful information to assess the quality of PCs. However, the variable effects of processing and storage on platelet activity need further exploration.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ajts.ajts_124_21","type":"journal-article","created":{"date-parts":[[2024,12,23]],"date-time":"2024-12-23T12:27:48Z","timestamp":1734956868000},"page":"197-202","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":1,"title":["Evaluating platelet concentrates by platelet indices, thromboelastography, and flow cytometry"],"prefix":"10.4103","volume":"18","author":[{"given":"Tapasyapreeti","family":"Mukhopadhyay","sequence":"first","affiliation":[{"name":"Departments of Laboratory Medicine, Jai Prakash Narayan Apex Trauma Centre, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arulselvi","family":"Subramanian","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine, Jai Prakash Narayan Apex Trauma Centre, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Venencia","family":"Albert","sequence":"additional","affiliation":[{"name":"Indian Council of Medical Research, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anand","family":"Kumar","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine, Jai Prakash Narayan Apex Trauma Centre, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tushar","family":"Sehgal","sequence":"additional","affiliation":[{"name":"Laboratory Medicine, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sulekha","family":"Karjee","sequence":"additional","affiliation":[{"name":"Transfusion Medicine, Jai Prakash Narayan Apex Trauma Centre, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harprasad","family":"Pati","sequence":"additional","affiliation":[{"name":"Department of Hematology, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2023,5,11]]},"reference":[{"key":"R1-20241224","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1046\/j.1537-2995.1984.24284173347.x","article-title":"Five-day storage of platelet concentrates","volume":"24","author":"Rock","year":"1984","journal-title":"Transfusion"},{"key":"R2-20241224","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.tmrv.2008.05.004","article-title":"Platelet storage lesion: A new understanding from a proteomic perspective","volume":"22","author":"Thon","year":"2008","journal-title":"Transf Med Rev"},{"key":"R4-20241224","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1007\/s12288-020-01349-x","article-title":"Platelet function analysis by flowcytometry in thrombocytopenic trauma patients","volume":"37","author":"Mukhopadhyay","year":"2021","journal-title":"Indian J Hematol Blood Transfus"},{"key":"R5-20241224","doi-asserted-by":"crossref","first-page":"231","DOI":"10.4103\/0377-4929.134676","article-title":"Establishing biological reference intervals for novel platelet parameters (immature platelet fraction, high immature platelet fraction, platelet distribution width, platelet large cell ratio, platelet-X, plateletcrit, and platelet distribution width) and their correlations among each other","volume":"57","author":"Sachdev","year":"2014","journal-title":"Indian J Pathol Microbiol"},{"key":"R6-20241224","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1309\/Y9EC63RW3XG1V313","article-title":"Platelet aggregation testing in platelet-rich plasma: Description of procedures with the aim to develop standards in the field","volume":"123","author":"Zhou","year":"2005","journal-title":"Am J Clin Pathol"},{"key":"R7-20241224","doi-asserted-by":"crossref","first-page":"S1","DOI":"10.1016\/S0268-960X(03)90000-2","article-title":"A cell-based model of coagulation and the role of factor VIIa","volume":"17","author":"Hoffman","year":"2003","journal-title":"Blood Rev"},{"key":"R8-20241224","doi-asserted-by":"crossref","first-page":"25","DOI":"10.5005\/jp-journals-10027-1006","article-title":"A review of thromboelastography","volume":"1","author":"Thakur","year":"2012","journal-title":"Int J Periop Ultrasound Appl Technol"},{"key":"R9-20241224","first-page":"13","article-title":"The induction of the release reaction in human blood platelets by close cell contact","volume":"25","author":"Massini","year":"1971","journal-title":"Thromb Diath Haemorrh"},{"key":"R10-20241224","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1159\/000504917","article-title":"Evaluation of the in vitro function of platelet concentrates from pooled buffy coats or apheresis","volume":"47","author":"Fiedler","year":"2020","journal-title":"Transfus Med Hemother"},{"key":"R11-20241224","first-page":"15","article-title":"Platelet activation in stored platelet concentrates: Comparison of two methods preparation","volume":"1","author":"Ali","year":"2012","journal-title":"J Hematol"},{"key":"R12-20241224","first-page":"207","article-title":"Evaluation of platelet aggregation in platelet concentrates: Storage implications","volume":"25","author":"Neiva","year":"2003","journal-title":"Rev Bras Hematol Hemoterapia"},{"key":"R13-20241224","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4103\/0973-6247.150933","article-title":"Platelet storage lesion: An update","volume":"9","author":"Mittal","year":"2015","journal-title":"Asian J Transf Sci"},{"key":"R14-20241224","doi-asserted-by":"crossref","first-page":"269","DOI":"10.3233\/CH-2011-1407","article-title":"Comparison of soluble CD40L concentrations and release capacities in apheresis and prestorage pooled platelet concentrates","volume":"47","author":"Wenzel","year":"2011","journal-title":"Clin Hemorheol Microcirc"},{"key":"R16-20241224","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.5858\/arpa.2013-0680-OA","article-title":"Use of a cytokine-release assay to demonstrate loss of platelet secretory capacity during blood bank processing and storage","volume":"138","author":"Tormey","year":"2014","journal-title":"Arch Pathol Lab Med"},{"key":"R17-20241224","doi-asserted-by":"crossref","first-page":"41","DOI":"10.4103\/0973-6247.150949","article-title":"Serial changes in morphology and biochemical markers in platelet preparations with storage","volume":"9","author":"Jain","year":"2015","journal-title":"Asian J Transfus Sci"},{"key":"R18-20241224","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1111\/voxs.12202","article-title":"Recent advances in platelet processing and storage","volume":"11","author":"Lozano","year":"2016","journal-title":"ISBT Sci Ser"}],"container-title":["Asian Journal of Transfusion Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/ajts.AJTS_124_21","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:00:21Z","timestamp":1786647621000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/ajts.AJTS_124_21"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,11]]},"references-count":16,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024]]}},"URL":"https:\/\/doi.org\/10.4103\/ajts.ajts_124_21","relation":{},"ISSN":["0973-6247","1998-3565"],"issn-type":[{"value":"0973-6247","type":"print"},{"value":"1998-3565","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,11]]},"assertion":[{"value":"2021-08-27","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-29","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}