{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T19:17:30Z","timestamp":1787771850077,"version":"build-2784847793"},"reference-count":9,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","license":[{"start":{"date-parts":[[2024,2,6]],"date-time":"2024-02-06T00:00:00Z","timestamp":1707177600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    Antibody identification is usually performed by antibody identification panels which are specially selected red cells, at least one of which has a homozygous expression for all clinically significant blood group antigens facilitating agglutination upon reaction with specific antibodies even exhibiting dosage. In general, adsorption elution is used in determining antibody specificities in the background of multiple alloantibodies with or without autoantibodies. Herein, we present a classic case where a routine antibody identification panel failed to detect a solo antibody from patient serum, following which we tested the eluate obtained after adsorption with a cross-match incompatible packed red blood cell unit using the same antibody identification panel, which unveiled the Fy\n                    <jats:sup>b<\/jats:sup>\n                    antibody.\n                  <\/jats:p>","DOI":"10.4103\/ajts.ajts_141_23","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T01:00:52Z","timestamp":1707267652000},"page":"150-153","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Identification of antibody specificity using adsorption-elution studies with incompatible packed red cell unit"],"prefix":"10.4103","volume":"19","author":[{"given":"Kella","family":"Nivedita","sequence":"first","affiliation":[{"name":"Department of Transfusion Medicine, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somnath","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debasish","family":"Mishra","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Namrata","family":"Datta","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ansuman","family":"Sahu","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satya","family":"Prakash","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2024,2,6]]},"reference":[{"key":"R1-20260422","doi-asserted-by":"crossref","first-page":"155","DOI":"10.21307\/immunohematology-2019-022","article-title":"Separation of multiple antibodies by adsorption with allogeneic red blood cells","volume":"33","author":"Ekema","year":"2019","journal-title":"Immunohematology"},{"key":"R3-20260422","doi-asserted-by":"crossref","first-page":"65","DOI":"10.4097\/kjae.2013.64.1.65","article-title":"A detection of unexpected blood antibody at the time of transfusion was needed, during the operation -a case report-","volume":"64","author":"Kim","year":"2013","journal-title":"Korean J Anesthesiol"},{"key":"R4-20260422","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1111\/trf.12070","article-title":"Antibody of undetermined specificity: Frequency, laboratory features, and natural history","volume":"53","author":"Liu","year":"2013","journal-title":"Transfusion"},{"key":"R5-20260422","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1111\/vox.12797","article-title":"Prevalence and laboratorial determinants of the clinical relevance of antibodies of undetermined specificity","volume":"114","author":"de Albuquerque da Veiga Conrado","year":"2019","journal-title":"Vox Sang"},{"key":"R6-20260422","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1111\/trf.15718","article-title":"The evanescence and persistence of RBC alloantibodies in blood donors","volume":"60","author":"Hauser","year":"2020","journal-title":"Transfusion"},{"key":"R7-20260422","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1111\/trf.12147","article-title":"Antibodies of undetermined significance: Nuisance or near miss?","volume":"53","author":"Tormey","year":"2013","journal-title":"Transfusion"},{"key":"R8-20260422","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/j.1365-3148.2012.01199.x","article-title":"Guidelines for pre-transfusion compatibility procedures in blood transfusion laboratories. British Committee for Standards in Haematology","volume":"23","author":"Milkins","year":"2013","journal-title":"Transfus Med"},{"key":"R9-20260422","doi-asserted-by":"crossref","first-page":"e28","DOI":"10.1093\/labmed\/lmw014","article-title":"Clinical utility of genotyping human erythrocyte antigens","volume":"47","author":"Wilkinson","year":"2016","journal-title":"Lab Med"},{"key":"R10-20260422","doi-asserted-by":"crossref","first-page":"1","DOI":"10.21307\/immunohematology-2019-167","article-title":"Performance of an automated solid-phase red cell adherence system compared with that of a manual gel microcolumn assay for the identification of antibodies eluted from red blood cells","volume":"27","author":"Finck","year":"2011","journal-title":"Immunohematology"}],"container-title":["Asian Journal of Transfusion Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/ajts.ajts_141_23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T18:17:54Z","timestamp":1787768274000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/ajts.ajts_141_23"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,6]]},"references-count":9,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.4103\/ajts.ajts_141_23","relation":{},"ISSN":["0973-6247","1998-3565"],"issn-type":[{"value":"0973-6247","type":"print"},{"value":"1998-3565","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,6]]},"assertion":[{"value":"2023-07-14","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-02","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}