{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T15:22:46Z","timestamp":1787844166435,"version":"build-2784847793"},"reference-count":8,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","license":[{"start":{"date-parts":[[2024,10,23]],"date-time":"2024-10-23T00:00:00Z","timestamp":1729641600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>Abstract:<\/jats:title>\n                  <jats:p>Here, we present a case report detailing a failed harvest from a 3-year-old child, a sibling-matched related donor for a patient with thalassemia undergoing ABO-incompatible hematopoietic stem cell transplant. The initial apheresis procedure, with the donor having an hematocrit of 29.4 and a peripheral blood CD34 count of 72 cells\/\u00b5l, resulted in a poor harvest, exhibiting an inadequate collection efficiency of 21% for the apheresis procedure. We here analyzed the factors that overt red cell abnormalities (low mean corpuscular volume [MCV]) in donor may account for failures to collect the circulating CD34+ cells by standard apheresis variables despite adequate mobilization. Individuals with low MCV who are at risk of stem cells harvest failure can be identified prior to apheresis, and poor harvest can be avoided by adjustment of the apheresis variable.<\/jats:p>","DOI":"10.4103\/ajts.ajts_91_24","type":"journal-article","created":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T06:04:57Z","timestamp":1778738697000},"page":"228-231","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Poor harvest of peripheral blood stem cells in donor with thalassemia trait"],"prefix":"10.4103","volume":"20","author":[{"given":"Anju Radhakrishna","family":"Kurup","sequence":"first","affiliation":[{"name":"Department of Transfusion Medicine, Malabar Cancer Centre, Postgraduate Institute of Oncology Science and Research, Thalassery, Kerala, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohandoss","family":"Murugesan","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine, Malabar Cancer Centre, Postgraduate Institute of Oncology Science and Research, Thalassery, Kerala, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandran K.","family":"Nair","sequence":"additional","affiliation":[{"name":"Department of Clinical Hematology, Malabar Cancer Centre, Postgraduate Institute of Oncology Science and Research, Thalassery, Kerala, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vineetha","family":"Raghavan","sequence":"additional","affiliation":[{"name":"Department of Clinical Hematology, Malabar Cancer Centre, Postgraduate Institute of Oncology Science and Research, Thalassery, Kerala, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sangeetha Keloth","family":"Nayanar","sequence":"additional","affiliation":[{"name":"Department of Oncopathology, Malabar Cancer Centre, Postgraduate Institute of Oncology Science and Research, Thalassery, Kerala, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2024,10,23]]},"reference":[{"key":"R1-20260529","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1111\/trf.13951","article-title":"Poor stem cell harvest may not always be related to poor mobilization:Lessons gained from a mobilization study in patients with \u03b2-thalassemia major","volume":"57","author":"Constantinou","year":"2017","journal-title":"Transfusion"},{"key":"R2-20260529","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1016\/j.bbmt.2011.10.002","article-title":"Peripheral blood CD34+cell enumeration as a predictor of apheresis yield:An analysis of more than 1,000 collections","volume":"18","author":"Gambell","year":"2012","journal-title":"Biol Blood Marrow Transplant"},{"key":"R3-20260529","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1038\/bmt.2010.194","article-title":"Optimal timing of G-CSF administration for effective autologous stem cell collection","volume":"46","author":"Kim","year":"2011","journal-title":"Bone Marrow Transplant"},{"key":"R4-20260529","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1111\/vox.13410","article-title":"Prediction of blood volume to be processed to achieve a target number of CD34+cells:Development, validation and implementation of a formula","volume":"118","author":"Garc\u00eda-Garc\u00eda","year":"2023","journal-title":"Vox Sang"},{"key":"R5-20260529","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1046\/j.1537-2995.1998.38111299056315.x","article-title":"Factors affecting the efficiency of collection of CD34-positive peripheral blood cells by a blood cell separator","volume":"38","author":"Ford","year":"1998","journal-title":"Transfusion"},{"key":"R6-20260529","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1002\/jca.2920010402","article-title":"Mononuclear cell (MNC) collection by continuous-flow centrifugation (CFC)","volume":"1","author":"Hester","year":"1983","journal-title":"J Clin Apher"},{"key":"R7-20260529","doi-asserted-by":"crossref","first-page":"1881","DOI":"10.1111\/trf.12579","article-title":"Harvesting autologous stem cells from a patient with red blood cell abnormalities of \u03b2-thalassemia intermedia","volume":"54","author":"Sanford","year":"2014","journal-title":"Transfusion"},{"key":"R8-20260529","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1111\/j.1537-2995.2012.03751.x","article-title":"Poor harvest of peripheral blood stem cell in donors with microcytic red blood cells","volume":"53","author":"Wang","year":"2013","journal-title":"Transfusion"}],"container-title":["Asian Journal of Transfusion Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/ajts.ajts_91_24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T15:12:45Z","timestamp":1787843565000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/ajts.ajts_91_24"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,23]]},"references-count":8,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.4103\/ajts.ajts_91_24","relation":{},"ISSN":["0973-6247","1998-3565"],"issn-type":[{"value":"0973-6247","type":"print"},{"value":"1998-3565","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,23]]},"assertion":[{"value":"2024-06-06","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-22","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}