{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T07:08:54Z","timestamp":1788419334068,"version":"build-2803163510"},"reference-count":34,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,1]]},"abstract":"Abstract<\/jats:title>\n \n Background:<\/jats:title>\n Rhuflex-F is a proprietary Ayurvedic herbo-mineral formulation clinically used to combat and relieve stiffness in joints and muscles, reduce edema, restore mobility, and also effective in relieving the symptoms of other autoimmune illnesses that lead to rheumatism.<\/jats:p>\n <\/jats:sec>\n \n Aims:<\/jats:title>\n \n The aim and objective of the research study is to evaluate the efficacy of Rhuflex-F against\n in vitro<\/jats:italic>\n protein denaturation and\n in vivo<\/jats:italic>\n Freund\u2019s adjuvant-induced arthritis in albino rats.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and methods:<\/jats:title>\n \n In vitro<\/jats:italic>\n inhibition of protein denaturation activity was carried out using bovine serum albumin. For\n in vivo<\/jats:italic>\n activity, arthritis was induced by complete Freund\u2019s adjuvant in albino rats. Rhuflex-F (135\u2013270 mg\/kg, po) was administered for 30\n th<\/jats:sup>\n days in arthritic rats, and effects were assessed on primary and secondary paw edema, on pain response, hematological, serum biochemical parameters (serum transaminases, alkaline phosphatase, urea, uric acid, and orosomucoid), and serum anti-oxidant parameters and adrenal ascorbic acid.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n Aqueous extract of Rhuflex-F showed\n in vitro<\/jats:italic>\n protein denaturation inhibitory activity in a dose-dependent manner. Rhuflex-F showed nonsignificant decrease in primary and secondary paw edema with reduced pain response, some reversal effects on hematological parameters such as white blood cell and red blood cell related parameters and serum orosomucoid and adrenal ascorbic acid in comparison to Fruend\u2019s adjuvant control group. Further, Rhuflex-F reversed Freund\u2019s adjuvant-induced adverse effects on oxidant status in the serum of albino rats.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n \n Result of the present study suggested that Rhuflex-F formulation has anti-inflammatory activity, may be due to the inhibition of protein denaturation\n in vitro<\/jats:italic>\n and\n in vivo<\/jats:italic>\n anti-arthritic activity against complete Freund\u2019s adjuvant-induced arthritis in albino rats.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/ayu.ayu_327_21","type":"journal-article","created":{"date-parts":[[2024,2,23]],"date-time":"2024-02-23T15:00:09Z","timestamp":1708700409000},"page":"30-37","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of the anti-arthritic activity of Rhuflex-F \u2013 A proprietary Ayurvedic herbomineral formulation in albino 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