{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T15:21:13Z","timestamp":1787757673478,"version":"build-2784847793"},"reference-count":32,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:sec>\n                    <jats:title>Background and Aim:<\/jats:title>\n                    <jats:p>\n                      The periodontal microbiome being complex, this study was aimed to detect and quantify the prevalence of\n                      <jats:italic toggle=\"yes\">Filifactor alocis<\/jats:italic>\n                      in various stages of periodontitis and to evaluate its prospect as a diagnostic marker for periodontal disease.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Settings and Design:<\/jats:title>\n                    <jats:p>Sixty subjects were selected (20 healthy controls, 20 with chronic periodontitis, and 20 with aggressive periodontitis) for the study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      Clinical parameters probing depth and the level of clinical attachment was recorded, subgingival plaque samples were collected. The\n                      <jats:italic toggle=\"yes\">F. alocis<\/jats:italic>\n                      16srDNA was cloned, sequenced, and used as the standard for real-time quantification of bacterial load using SYBR green chemistry.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Statistical Analysis:<\/jats:title>\n                    <jats:p>Clinical, microbiological, and quantitative polymerase chain reaction (PCR) data were analyzed using ANOVA and Pearson's coefficient correlation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      (a) Real-time PCR analysis showed the highest average\n                      <jats:italic toggle=\"yes\">F. alocis<\/jats:italic>\n                      count in chronic periodontitis subjects (32,409.85), which was followed by count in healthy controls (3046.15) and the least count in aggressive periodontitis subjects (939.84). The bacterial count was statistically significant at\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.005. (b) An intra-group comparison reveals that there was a statistically significant increase in the bacterial count with age and mean probing pocket depth at\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.0005.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">F. alocis<\/jats:italic>\n                      population in aggressive periodontitis was lower compared to chronic periodontitis and healthy controls. The\n                      <jats:italic toggle=\"yes\">F. alocis<\/jats:italic>\n                      population surge in healthy controls may be due to geographical variations and the ethnicity of the subjects. A higher population of\n                      <jats:italic toggle=\"yes\">F. alocis<\/jats:italic>\n                      in chronic periodontitis proves its high pathogenic potential to invade the host tissues to aid in further periodontal destruction.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ccd.ccd_803_20","type":"journal-article","created":{"date-parts":[[2021,12,21]],"date-time":"2021-12-21T13:16:11Z","timestamp":1640092571000},"page":"433-438","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":9,"title":["The Varied Proportion of Filifactor alocis in Periodontal Health and Disease in the South Indian Subpopulation"],"prefix":"10.4103","volume":"12","author":[{"given":"Anila","family":"Neelakandan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravishankar","family":"Potluri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pradeep Kumar","family":"Yadalam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priyankar","family":"Chakraborty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. V.","family":"Saravanan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rex","family":"Arunraj","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-16-20240901","doi-asserted-by":"crossref","first-page":"149","DOI":"10.4103\/0974-777X.56251","article-title":"Periodontitis, a true infection","volume":"1","author":"Saini","year":"2009","journal-title":"J Glob Infect Dis"},{"key":"R3-16-20240901","first-page":"65","article-title":"Chemotherapy of dental plaque infections","volume":"9","author":"Loesche","year":"1976","journal-title":"Oral Sci Rev"},{"key":"R4-16-20240901","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1177\/08959374940080022001","article-title":"Microbial ecology of dental plaque and its significance in health and disease","volume":"8","author":"Marsh","year":"1994","journal-title":"Adv Dent Res"},{"key":"R5-16-20240901","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1038\/nrmicro2873","article-title":"The keystone-pathogen hypothesis","volume":"10","author":"Hajishengallis","year":"2012","journal-title":"Nat Rev Microbiol"},{"key":"R6-16-20240901","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1111\/j.2041-1014.2012.00663.x","article-title":"Beyond the red complex and into more complexity: The polymicrobial synergy and dysbiosis (PSD) model of periodontal disease etiology","volume":"27","author":"Hajishengallis","year":"2012","journal-title":"Mol Oral Microbiol"},{"key":"R7-16-20240901","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.it.2013.09.001","article-title":"Immunomicrobial pathogenesis of periodontitis: keystones, pathobionts, and host response","volume":"35","author":"Hajishengallis","year":"2014","journal-title":"Trends Immunol"},{"key":"R8-16-20240901","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.imlet.2014.08.017","article-title":"The oral microbiome and the immunobiology of periodontal disease and caries","volume":"162","author":"Costalonga","year":"2014","journal-title":"Immunol Lett"},{"key":"R9-16-20240901","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/j.1600-051X.2010.01679.x","article-title":"Has the use of molecular methods for the characterization of the human oral microbiome changed our understanding of the role of bacteria in the pathogenesis of periodontal disease?","volume":"38","author":"Wade","year":"2011","journal-title":"J Clin Periodontol"},{"key":"R10-16-20240901","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1902\/annals.1999.4.1.1","article-title":"Development of classification system for periodontal diseases and conditions","volume":"4","author":"Armitage","year":"1999","journal-title":"Ann Periodontol"},{"key":"R11-16-20240901","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1034\/j.1399-302X.1999.140510.x","article-title":"Sampling of periodontal pathogens by paper points: Evaluation of basic parameters","volume":"14","author":"Hartroth","year":"1999","journal-title":"Oral Microbiol Immunol"},{"key":"R12-16-20240901","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/S0002-8177(91)26016-X","article-title":"Variations in the prevalence and extent of periodontitis","volume":"122","author":"Oliver","year":"1991","journal-title":"J Am Dent Assoc"},{"key":"R13-16-20240901","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1177\/0022034512457373","article-title":"CDC Periodontal Disease Surveillance workgroup: James Beck (University of North Carolina, Chapel Hill, USA), Gordon Douglass (Past President, American Academy of Periodontology), Roy Page (University of Washin). Prevalence of periodontitis in adults in the United States: 2009 and 2010","volume":"91","author":"Eke","year":"2012","journal-title":"J Dent Res"},{"key":"R14-16-20240901","first-page":"129","article-title":"Periodontitis among adults aged >\/=30 years - United States, 2009-2010","volume":"62","author":"Thornton-Evans","year":"2013","journal-title":"MMWR Surveill Summ"},{"key":"R15-16-20240901","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1177\/154405910308200503","article-title":"New bacterial species associated with chronic periodontitis","volume":"82","author":"Kumar","year":"2003","journal-title":"J Dent Res"},{"key":"R16-16-20240901","doi-asserted-by":"crossref","first-page":"3944","DOI":"10.1128\/JCM.43.8.3944-3955.2005","article-title":"Identification of candidate periodontal pathogens and beneficial species by quantitative 16S clonal analysis","volume":"43","author":"Kumar","year":"2005","journal-title":"J Clin Microbiol"},{"key":"R17-16-20240901","doi-asserted-by":"crossref","first-page":"3665","DOI":"10.1128\/JCM.00317-06","article-title":"Changes in periodontal health status are associated with bacterial community shifts as assessed by quantitative 16S cloning and sequencing","volume":"44","author":"Kumar","year":"2006","journal-title":"J Clin Microbiol"},{"key":"R18-16-20240901","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1099\/00207713-35-4-475","article-title":"Fusobacterium alocis sp. nov. and Fusobacterium sulci sp. nov. from the human gingival sulcus","volume":"35","author":"Cato","year":"1985","journal-title":"Int J Syst Bacteriol"},{"key":"R19-16-20240901","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1128\/jcm.34.2.376-384.1996","article-title":"Rapid characteri-zation of periodontal bacterial isolates by using flurogenic substrate tests","volume":"34","author":"Maiden","year":"1996","journal-title":"J Clin Microbiol"},{"key":"R20-16-20240901","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1111\/j.1399-302X.2005.00243.x","article-title":"A new checkerboard panel for testing bacterial markers in periodontal disease","volume":"21","author":"Dahl\u00e9n","year":"2006","journal-title":"Oral Microbiol Immunol"},{"key":"R21-16-20240901","doi-asserted-by":"crossref","first-page":"3770","DOI":"10.1128\/JB.183.12.3770-3783.2001","article-title":"Bacterial diversity in human subgingival plaque","volume":"183","author":"Paster","year":"2001","journal-title":"J Bacteriol"},{"key":"R22-16-20240901","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1186\/1471-2180-10-66","article-title":"Filifactor alocis Involvement in periodontal biofilms","volume":"10","author":"Schlafer","year":"2010","journal-title":"BMC Microbiol"},{"key":"R23-16-20240901","doi-asserted-by":"crossref","first-page":"143","DOI":"10.5051\/jkape.2008.38.2.143","article-title":"Identification of putative periodontal pathogens in Korean chronic periodontitis patients","volume":"38","author":"Yun","year":"2008","journal-title":"J Korean Acad Periodontol"},{"key":"R24-16-20240901","doi-asserted-by":"crossref","first-page":"E13646","DOI":"10.5812\/jjm.13646","article-title":"Identification of bacteria associated with a periodontal disease in Thai patients based on next-generation sequencing","volume":"10","author":"Payungporn","year":"2017","journal-title":"Jundishapur J Microbiol"},{"key":"R25-16-20240901","doi-asserted-by":"crossref","first-page":"33638","DOI":"10.1038\/srep33638","article-title":"In situ anabolic activity of periodontal pathogens Porphyromonas gingivalis and Filifactor alocis in chronic periodontitis","volume":"6","author":"Spooner","year":"2016","journal-title":"Sci Rep"},{"key":"R26-16-20240901","doi-asserted-by":"crossref","first-page":"3872","DOI":"10.1128\/IAI.05631-11","article-title":"Filifactor alocis has virulence attributes that can enhance its persistence under oxidative stress conditions and mediate invasion of epithelial cells by Porphyromonas gingivalis","volume":"79","author":"Aruni","year":"2011","journal-title":"Infect Immun"},{"key":"R27-16-20240901","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1111\/jre.12210","article-title":"Quantitative analysis of classical and new putative periodontal pathogens in subgingival biofilm: A case-control study","volume":"50","author":"Al-hebshi","year":"2015","journal-title":"J Periodontal Res"},{"key":"R28-16-20240901","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1186\/2049-2618-2-32","article-title":"Bacterial community composition of chronic periodontitis and novel oral sampling sites for detecting disease indicators","volume":"2","author":"Galimanas","year":"2014","journal-title":"Microbiome"},{"key":"R29-16-20240901","doi-asserted-by":"crossref","first-page":"2850","DOI":"10.1128\/JCM.00729-13","article-title":"A consortium of Aggregatibacter actinomycetemcomitans (Aa), Streptococcus parasanguinis and Filifactor alocis are present in sites prior to bone loss in a longitudinal study of localized aggressive periodontitis","volume":"51","author":"Fine","year":"2013","journal-title":"J Clin Microbiol"},{"key":"R30-16-20240901","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1034\/j.1600-0757.2002.290101.x","article-title":"Global epidemiology of periodontal diseases: An overview","volume":"29","author":"Albandar","year":"2002","journal-title":"Periodontology 2000"},{"key":"R31-16-20240901","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1111\/j.1600-051X.2005.00637.x","article-title":"Occurrence and risk indicators of increased probing depth in an adult Brazilian population","volume":"32","author":"Susin","year":"2005","journal-title":"J Clin Periodontol"},{"key":"R32-16-20240901","doi-asserted-by":"crossref","first-page":"e76271","DOI":"10.1371\/journal.pone.0076271","article-title":"Oral community interactions of Filifactor alocis in vitro","volume":"8","author":"Wang","year":"2013","journal-title":"PLoS One"},{"key":"R33-16-20240901","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1902\/jop.2007.060187","article-title":"Demographic, clinical, and microbial aspects of chronic and aggressive periodontitis in Colombia: A multicenter study","volume":"78","author":"Lafaurie","year":"2007","journal-title":"J Periodontol"}],"container-title":["Contemporary Clinical Dentistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/ccd.ccd_803_20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T14:49:41Z","timestamp":1787755781000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/ccd.ccd_803_20"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10]]},"references-count":32,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021]]}},"URL":"https:\/\/doi.org\/10.4103\/ccd.ccd_803_20","relation":{},"ISSN":["0976-237X"],"issn-type":[{"value":"0976-237X","type":"print"}],"subject":[],"published":{"date-parts":[[2021,10]]}}}