{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T15:25:48Z","timestamp":1787757948592,"version":"build-2784847793"},"reference-count":32,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      This study aimed to assess (1) the reproducibility of three sperm chromatin dispersion (SCD) assays for sperm DNA fragmentation, i.e., LensHooke R10\n                      <jats:sup>\u00ae<\/jats:sup>\n                      (R10), Halosperm G2\n                      <jats:sup>\u00ae<\/jats:sup>\n                      (G2), and BASO\n                      <jats:sup>\u00ae<\/jats:sup>\n                      (BA); (2) the correlation between computer-assisted semen analyzer (CASA) morphokinematic parameters and sperm DNA fragmentation index (DFI), and (3) the diagnostic value for male reproduction by combining semen morphokinematic parameters and DFI. Total 50 male participants were recruited, and all collected semen samples underwent semen analyses and SCD assays. Intra- and inter-observer variability of DFI data from different SCD measures was tested. In addition, the predictive ability of CASA parameters and DFI (with different cutoffs, i.e., 15% and 20%) for infertility was assessed using receiver operating characteristic curve analysis. We found that the G2 and R10 produced satisfactory variance coefficients (5.53%, 5.67%) compared to BA (14.8%). The DFI data from the R10 had lower intra-observer variability, in terms of higher intra-class coefficient (0.9615), than that of the G2 (0.8847) or BA (0.8824). Inter-observer variability of three SCD kits in scoring the DFI was comparable and satisfactory (concordance correlation coefficients ranging 0.9895\u20130.9630). The CASA parameters (i.e., total motility [\n                      <jats:italic toggle=\"yes\">r<\/jats:italic>\n                      = \u22120.57], progression motility [\n                      <jats:italic toggle=\"yes\">r<\/jats:italic>\n                      = \u22120.55], and rapidly progressive motility [\n                      <jats:italic toggle=\"yes\">r<\/jats:italic>\n                      = \u22120.55]) were significantly correlated with DFI (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.001). The predictive ability of the 15%-cutoff DFI data was better than that of the 20%-cutoff or continuous DFI data. The model comprising the CASA parameters, 15%-cutoff DFI, and 4%-cutoff normal morphology had the highest area under curve (0.8125) for infertility. For SCD assay, the R10 was the most reliable SCD assay to detect sperm DNA fragmentation. Combining the sperm DFI with CASA parameters might be a better diagnostic tool for male reproduction.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/cjp.cjp_12_22","type":"journal-article","created":{"date-parts":[[2022,6,27]],"date-time":"2022-06-27T01:28:32Z","timestamp":1656293312000},"page":"143-150","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":13,"title":["Incorporating Sperm DNA Fragmentation Index with Computer-Assisted Semen Morphokinematic Parameters as a Better Window to Male Fertility"],"prefix":"10.4103","volume":"65","author":[{"given":"Hsin-Ta","family":"Lin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng-Hsing","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weng-Ling","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Chung","family":"Tsai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Ying","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuei-Hsiang","family":"Hung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Po-Hsuan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ta-Sheng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huang-Tz","family":"Ou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Sheng","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-6-20240830","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.fertnstert.2006.02.129","article-title":"Declining estimates of infertility in the United States: 1982-2002","volume":"86","author":"Stephen","year":"2006","journal-title":"Fertil Steril"},{"key":"R3-6-20240830","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1093\/biolre\/iox120","article-title":"CASAnova: A multiclass support vector machine model for the classification of human sperm motility patterns","volume":"97","author":"Goodson","year":"2017","journal-title":"Biol Reprod"},{"key":"R5-6-20240830","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1007\/s11255-015-1059-0","article-title":"A systematic review of recent clinical practice guidelines and best practice statements for the evaluation of the infertile male","volume":"47","author":"Esteves","year":"2015","journal-title":"Int Urol Nephrol"},{"key":"R6-6-20240830","doi-asserted-by":"crossref","first-page":"2908","DOI":"10.1093\/humrep\/des261","article-title":"The effect of sperm DNA fragmentation on miscarriage rates: A systematic review and meta-analysis","volume":"27","author":"Robinson","year":"2012","journal-title":"Hum Reprod"},{"key":"R7-6-20240830","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.fertnstert.2019.03.003","article-title":"Sperm DNA fragmentation and recurrent pregnancy loss: A systematic review and meta-analysis","volume":"112","author":"McQueen","year":"2019","journal-title":"Fertil Steril"},{"key":"R8-6-20240830","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1093\/humrep\/deab004","article-title":"Should we be measuring DNA damage in human spermatozoa? 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