{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T12:13:36Z","timestamp":1788783216669,"version":"build-2803163510"},"reference-count":33,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"7","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"abstract":"ABSTRACT<\/jats:title>\n \n Background:<\/jats:title>\n Coronal microleakage compromises the longevity of root-filled teeth. Intra-orifice barriers are used to reduce coronal ingress, but comparative performance among bioceramics remains uncertain. This study aimed to evaluate the coronal microleakage of two bioceramic materials, NeoPutty and mineral trioxide aggregate (MTA) Plus, used as intraorifice barriers in extracted human single-rooted premolars.<\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n This randomized controlled\n in vitro<\/jats:italic>\n experimental study included 50 extracted human single-rooted premolars, which were randomly allocated into four groups: two experimental groups (NeoPutty and MTA Plus;\n n<\/jats:italic>\n = 20 each) and two control groups (positive and negative controls;\n n<\/jats:italic>\n = 5 each). Following root canal preparation and obturation, intraorifice barriers were placed, and the samples were immersed in Rhodamine B dye for 48 h. The teeth were then sectioned buccolingually, and dye penetration was observed under a stereomicroscope (\u00d716). Data were analyzed using an independent samples\n t<\/jats:italic>\n -test with a significance level of\n P<\/jats:italic>\n < 0.05.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n The mean dye penetration with standard deviation (SD) was 5.49 (1.72) mm for NeoPutty and 5.21 (1.11) mm for MTA Plus; the difference was not statistically significant (NeoPutty \u2212 MTA Plus = 0.275 mm; 95% confidence interval [CI], \u20130.651\u20131.201;\n t<\/jats:italic>\n = 0.60;\n P<\/jats:italic>\n = 0.55).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n Both NeoPutty and MTA Plus showed similar coronal sealing effectiveness as intraorifice barriers in premolars. These results support the use of either material to enhance the coronal seal. Further studies incorporating aging protocols and alternative leakage models are recommended.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/drj.drj_75_26","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T12:00:18Z","timestamp":1788782418000},"update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Coronal microleakage of two intraorifice bioceramic barriers in single-rooted premolars: In vitro study"],"prefix":"10.4103","volume":"23","author":[{"given":"Saad","family":"Aslam","sequence":"first","affiliation":[{"name":"Department of Restorative Dental Science, College of Dentistry, Gulf Medical University, Ajman, UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heba Mohamed","family":"Abdelaal","sequence":"additional","affiliation":[{"name":"Department of Restorative Dental Science, College of Dentistry, Gulf Medical University, Ajman, UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarek M.","family":"Elsewify","sequence":"additional","affiliation":[{"name":"Department of Restorative Dental Science, College of Dentistry, Gulf Medical University, Ajman, UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bassem M.","family":"Eid","sequence":"additional","affiliation":[{"name":"Department of Restorative Dental Science, College of Dentistry, Gulf Medical University, Ajman, UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranya F.","family":"Elemam","sequence":"additional","affiliation":[{"name":"Department of Restorative Dental Science, College of Dentistry, Gulf Medical University, Ajman, UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2026,7]]},"reference":[{"key":"R1-20260907","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/S0099-2399(06)80533-9","article-title":"Human saliva penetration of coronally unsealed obturated root canals","volume":"19","author":"Khayat","year":"1993","journal-title":"J Endod"},{"key":"R2-20260907","doi-asserted-by":"crossref","first-page":"466","DOI":"10.3390\/polym14030466","article-title":"An in-vitro evaluation of microleakage in resin-based restorative materials at different time intervals","volume":"14","author":"Bilgrami","year":"2022","journal-title":"Polymers (Basel)"},{"key":"R3-20260907","first-page":"99","article-title":"Microleakage in endodontics","volume":"6","author":"Muliyar","year":"2014","journal-title":"J Int Oral Health"},{"key":"R4-20260907","first-page":"e812","article-title":"Importance and methodologies of endodontic microleakage studies: A systematic review","volume":"9","author":"Jafari","year":"2017","journal-title":"J Clin Exp Dent"},{"key":"R5-20260907","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1067\/mpr.2002.124453","article-title":"Endodontic failure caused by inadequate restorative procedures: Review and treatment recommendations","volume":"87","author":"Heling","year":"2002","journal-title":"J Prosthet Dent"},{"key":"R6-20260907","doi-asserted-by":"crossref","first-page":"672887","DOI":"10.3389\/froh.2021.672887","article-title":"Microbiological aspects of root canal infections and disinfection strategies: An update review on the current knowledge and challenges","volume":"2","author":"Wong","year":"2021","journal-title":"Front Oral Health"},{"key":"R7-20260907","doi-asserted-by":"crossref","first-page":"144","DOI":"10.4103\/1305-7456.175682","article-title":"Failure of endodontic treatment: The usual suspects","volume":"10","author":"Tabassum","year":"2016","journal-title":"Eur J Dent"},{"key":"R8-20260907","doi-asserted-by":"crossref","first-page":"18","DOI":"10.14744\/eej.2023.76598","article-title":"The study and relevance of pulp chamber anatomy in endodontics-a comprehensive review","volume":"9","author":"Ahmed","year":"2024","journal-title":"Eur Endod J"},{"key":"R9-20260907","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1055\/s-0040-1713073","article-title":"Mineral trioxide aggregate applications in endodontics: A review","volume":"14","author":"Cervino","year":"2020","journal-title":"Eur J Dent"},{"key":"R10-20260907","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1111\/adj.12951","article-title":"Orifice barriers to prevent coronal microleakage after root canal treatment: Systematic review and meta-analysis","volume":"68","author":"Chen","year":"2023","journal-title":"Aust Dent J"},{"key":"R11-20260907","first-page":"274","article-title":"Apical microleakage in root canals with separated rotary instruments obturated with different endodontic sealers","volume":"25","author":"Manjila","year":"2022","journal-title":"J Conserv Dent"},{"key":"R12-20260907","doi-asserted-by":"crossref","first-page":"286","DOI":"10.4103\/JCDE.JCDE_314_23","article-title":"Retreatability of NeoSEALER Flo obturated with warm vertical compaction versus single-cone technique using two different retreatment systems","volume":"27","author":"Elzanaty","year":"2024","journal-title":"J Conserv Dent Endod"},{"key":"R13-20260907","doi-asserted-by":"crossref","first-page":"491","DOI":"10.4103\/JCDE.JCDE_103_24","article-title":"Evaluation of the antibacterial and cytotoxic properties of TotalFill and NeoSEALER Flo bioceramic sealers","volume":"27","author":"Sebastian","year":"2024","journal-title":"J Conserv Dent Endod"},{"key":"R14-20260907","doi-asserted-by":"crossref","first-page":"199","DOI":"10.4103\/JCDE.JCDE_605_24","article-title":"Sealing ability of three bioceramic sealers using sealer-based obturation method after immersion in simulated body fluid","volume":"28","author":"Singhal","year":"2025","journal-title":"J Conserv Dent Endod"},{"key":"R15-20260907","first-page":"500","article-title":"Antimicrobial properties of hydraulic bioceramic calcium silicate-based cements","volume":"18","author":"Hammad","year":"2025","journal-title":"J Int Dent Med Res"},{"key":"R16-20260907","doi-asserted-by":"crossref","first-page":"354","DOI":"10.3390\/bioengineering10030354","article-title":"Bioceramics in endodontics: Updates and future perspectives","volume":"10","author":"Dong","year":"2023","journal-title":"Bioengineering (Basel)"},{"key":"R17-20260907","first-page":"122","article-title":"Evaluation of the effect of different intraorifice barrier materials on coronal microleakage of endodontically treated teeth using micro-computed tomography technology: A comparative in vitro study","volume":"(340-341)","author":"Abdullah","year":"2023","journal-title":"Georgian Med News"},{"key":"R18-20260907","first-page":"156","article-title":"An in vitro comparison of coronal microleakage of three orifice barriers filling materials","volume":"7","author":"Yavari","year":"2012","journal-title":"Iran Endod J"},{"key":"R19-20260907","first-page":"2789073","article-title":"Effect of an intraorifice barrier on endodontically treated teeth: a systematic review and meta-analysis of In vitro studies","volume":"(1)","author":"Ara\u00fajo","year":"2022","journal-title":"BioMed Research International. 2022"},{"key":"R20-20260907","doi-asserted-by":"crossref","first-page":"259","DOI":"10.4103\/ccd.ccd_253_24","article-title":"Stereomicroscopic evaluation of sealing ability of three different furcal perforation repair materials: An in vitro study","volume":"15","author":"De","year":"2024","journal-title":"Contemp Clin Dent"},{"key":"R21-20260907","first-page":"1183","article-title":"Calcium silicate-based cements in endodontics","volume":"13","author":"Kunjan","year":"2020","journal-title":"J Int Dent Med Res"},{"key":"R22-20260907","first-page":"416","article-title":"Sealing ability of three different materials to repair furcation perforations using computerized fluid filtration method","volume":"47","author":"Ko\u00e7","year":"2021","journal-title":"Aust Endod J"},{"key":"R23-20260907","doi-asserted-by":"crossref","first-page":"493","DOI":"10.4103\/jispcd.JISPCD_68_23","article-title":"Comparative evaluation of the antimicrobial activity of NeoPutty MTA and modified NeoPutty MTA: An in vitro study","volume":"13","author":"Acharya","year":"2023","journal-title":"J Int Soc Prev Community Dent"},{"key":"R24-20260907","doi-asserted-by":"crossref","first-page":"588","DOI":"10.4103\/jcd.jcd_377_22","article-title":"Evaluation of coronal microleakage of intra-orifice barrier materials in endodontically treated teeth: A systematic review","volume":"25","author":"Mehta","year":"2022","journal-title":"J Conserv Dent"},{"key":"R25-20260907","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.jobcr.2021.03.001","article-title":"In-vitro evaluation of microleakage of bioceramic root-end filling materials: A spectrophotometric study","volume":"11","author":"Gupta","year":"2021","journal-title":"J Oral Biol Craniofac Res"},{"key":"R26-20260907","doi-asserted-by":"crossref","first-page":"817","DOI":"10.4103\/JCDE.JCDE_264_24","article-title":"Volumetric change of calcium silicate-based repair materials in a simulated inflammatory environment: A micro-computed tomography study","volume":"27","author":"Mendon\u00e7a","year":"2024","journal-title":"J Conserv Dent Endod"},{"key":"R27-20260907","doi-asserted-by":"crossref","first-page":"19244","DOI":"10.1038\/s41598-025-03729-7","article-title":"Marginal adaptation and porosity of calcium silicate-based cements in furcation perforations: A micro-CT comparative study","volume":"15","author":"Rojo-Carpintero","year":"2025","journal-title":"Sci Rep"},{"key":"R28-20260907","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1111\/aej.12415","article-title":"Coronal bacterial penetration after 7 days in class II endodontic access cavities restored with two temporary restorations: A randomised clinical trial","volume":"46","author":"Shanmugam","year":"2020","journal-title":"Aust Endod J"},{"key":"R29-20260907","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1186\/s12938-024-01290-4","article-title":"Comprehensive review of composition, properties, clinical applications, and future perspectives of calcium-enriched mixture (CEM) cement: A systematic analysis","volume":"23","author":"Asgary","year":"2024","journal-title":"Biomed Eng Online"},{"key":"R30-20260907","doi-asserted-by":"crossref","first-page":"e70044","DOI":"10.1111\/eos.70044","article-title":"Sealing ability of mineral trioxide aggregate: A scoping review of laboratory assessment methods","volume":"133","author":"Tsuchiya","year":"2025","journal-title":"Eur J Oral Sci"},{"key":"R31-20260907","doi-asserted-by":"crossref","first-page":"5607","DOI":"10.1039\/D2CS00799A","article-title":"Fluorescent dyes based on rhodamine derivatives for bioimaging and therapeutics: Recent progress, challenges, and prospects","volume":"52","author":"Zeng","year":"2023","journal-title":"Chem Soc Rev"},{"key":"R32-20260907","doi-asserted-by":"crossref","first-page":"S659","DOI":"10.4103\/jpbs.jpbs_921_23","article-title":"Comparative evaluation of sealing ability of three different materials as barriers to coronal microleakage in root-filled teeth: An in vitro study","volume":"16","author":"Jain","year":"2024","journal-title":"J Pharm Bioallied Sci"},{"key":"R33-20260907","doi-asserted-by":"crossref","first-page":"3174","DOI":"10.3390\/ma16083174","article-title":"Rheological properties and setting kinetics of bioceramic hydraulic cements: ProRoot MTA versus RS","volume":"16","author":"Jevnikar","year":"2023","journal-title":"Materials (Basel)"}],"container-title":["Dental Research Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/drj.drj_75_26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T12:00:22Z","timestamp":1788782422000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/drj.drj_75_26"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":33,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.4103\/drj.drj_75_26","relation":{},"ISSN":["1735-3327","2008-0255"],"issn-type":[{"value":"1735-3327","type":"print"},{"value":"2008-0255","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"2026-03-10","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-20","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}