{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T10:32:04Z","timestamp":1789813924009,"version":"4.0.1"},"reference-count":22,"publisher":"Georg Thieme Verlag KG","issue":"03","license":[{"start":{"date-parts":[[2019,9,25]],"date-time":"2019-09-25T00:00:00Z","timestamp":1569369600000},"content-version":"vor","delay-in-days":816,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Dent"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>\n          Objective:This study aims to assess the role of herpesviruses in chronic periodontitis and their association with clinical parameters and in increasing severity. Materials and Methods: This was a prospective case\u2013control study. Ethical approval and prior consent were taken. A subgingival plaque sample was collected from a total of 300 patients and 300 controls and processed for DNA extraction and multiplex polymerase chain reaction for detection of herpesviruses.Results: The most predominant age group affected was 41\u201350 followed by 31\u201340 years and male patients outnumbered the female patients. Herpes simplex virus (HSV)-1 (46.6%) was the most common Herpesvirus followed by HSV-2 (34.6%), Epstein\u2013Barr viruses (EBV) (30.6%), and cytomegalovirus (CMV) (19.3%) in chronic periodontitis. Herpesviruses were significantly associated with increasing severity of the disease and had shown differences in their association with clinical parameters. Multiple herpesvirus infection was seen in patients with severe chronic periodontitis. The most common combination was HSV-1 + HSV-2 and HSV-1 + HSV-2 + EBV. Conclusions: HSV-1 was the most common herpesviruses implicated in the etiology of the chronic periodontitis followed by HSV-2, EBV and CMV. A herpesvirus differs in association with clinical parameters and plays an important role in increasing severity of the disease.<\/jats:p>","DOI":"10.4103\/ejd.ejd_43_17","type":"journal-article","created":{"date-parts":[[2018,8,17]],"date-time":"2018-08-17T04:59:29Z","timestamp":1534481969000},"page":"299-304","source":"Crossref","is-referenced-by-count":30,"title":["Role of herpesviruses in chronic periodontitis and their association with clinical parameters and in increasing severity of the disease"],"prefix":"10.1055","volume":"11","author":[{"given":"Mohammad Mukhit Abdul Gaffar","family":"Kazi","sequence":"additional","affiliation":[{"name":"Department of General Pathology and Microbiology, Sinhgad Dental College and Hospital, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renu","family":"Bharadwaj","sequence":"additional","affiliation":[{"name":"Department of Microbiology, B. J. Government Medical College, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2019,9,25]]},"reference":[{"key":"key-10.4103\/1305-7456.213244-1","first-page":"133","volume-title":"Oral health status and practices of dentate Bhil adult tribes of southern Rajasthan, India","author":"Kumar","year":"2009","journal-title":"Int Dent J"},{"key":"key-10.4103\/1305-7456.213244-2","first-page":"373","volume-title":"Studies of the microbiology of periodontosis","author":"Newman","year":"1976","journal-title":"J Periodontol"},{"key":"key-10.4103\/1305-7456.213244-3","first-page":"1479","volume-title":"Association between human herpesviruses and the severity of periodontitis","author":"Ling","year":"2004","journal-title":"J Periodontol"},{"key":"key-10.4103\/1305-7456.213244-4","first-page":"289","volume-title":"Detection of human viruses in periodontal pockets using polymerase chain reaction","author":"Parra","year":"1996","journal-title":"Oral Microbiol Immunol"},{"key":"key-10.4103\/1305-7456.213244-5","first-page":"79","volume-title":"Herpesviruses in chronic and aggressive periodontitis patients in an Indian population","author":"Bilichodmath","year":"2009","journal-title":"J Oral Sci"},{"key":"key-10.4103\/1305-7456.213244-6","first-page":"1","volume-title":"Association of viruses in chronic periodontitis \u2013 Fact or hype","author":"Kolliyavar","year":"2013","journal-title":"Int J Oral Hyg Dent Res"},{"key":"key-10.4103\/1305-7456.213244-7","first-page":"1001","volume-title":"Detection and typing of HSV-1, HSV-2, CMV and EBV by quadruplex PCR","author":"Shin","year":"2003","journal-title":"Yonsei Med J"},{"key":"key-10.4103\/1305-7456.213244-8","first-page":"3","volume-title":"Herpesviruses in human periodontal disease","author":"Contreras","year":"2000","journal-title":"J Periodontal Res"},{"key":"key-10.4103\/1305-7456.213244-9","first-page":"206","volume-title":"Herpesvirus infection of inflammatory cells in human periodontitis","author":"Contreras","year":"1999","journal-title":"Oral Microbiol Immunol"},{"key":"key-10.4103\/1305-7456.213244-10","first-page":"301","volume-title":"Human cytomegalovirus escape from immune detection","author":"Michelson","year":"1999","journal-title":"Intervirology"},{"key":"key-10.4103\/1305-7456.213244-11","first-page":"131","volume-title":"Molecular pathways in virus-induced cytokine production","author":"Mogensen","year":"2001","journal-title":"Microbiol Mol Biol Rev"},{"key":"key-10.4103\/1305-7456.213244-12","first-page":"2313","volume-title":"Detection of herpesviruses and periodontal pathogens in subgingival plaque of patients with chronic periodontitis, generalized aggressive periodontitis, or gingivitis","author":"Imbronito","year":"2008","journal-title":"J Periodontol"},{"key":"key-10.4103\/1305-7456.213244-13","first-page":"1917","volume-title":"EBV and CMV in chronic periodontitis: A prevalence study","author":"Chalabi","year":"2008","journal-title":"Arch Virol"},{"key":"key-10.4103\/1305-7456.213244-14","first-page":"928","volume-title":"Low prevalence of subgingival viruses in periodontitis patients","author":"Nibali","year":"2009","journal-title":"J Clin Periodontol"},{"key":"key-10.4103\/1305-7456.213244-15","first-page":"13","volume-title":"Detection of herpes simplex virus (Hsv-1 & 2) in gingival tissue of chronic periodontitis patients using Polymerase Chain Reaction (PCR) technique","author":"Singh","year":"2014","journal-title":"J Periodontal Med Clin Pract"},{"key":"key-10.4103\/1305-7456.213244-16","first-page":"203","volume-title":"Detection of human herpes viruses in patients with chronic and aggressive periodontitis and relationship between viruses and clinical parameters","author":"Das","year":"2012","journal-title":"J Oral Maxillofac Pathol","ISSN":"https:\/\/id.crossref.org\/issn\/0973-029X","issn-type":"print"},{"key":"key-10.4103\/1305-7456.213244-17","first-page":"381","volume-title":"Mammalian viruses in human periodontitis","author":"Contreras","year":"1996","journal-title":"Oral Microbiol Immunol"},{"key":"key-10.4103\/1305-7456.213244-18","first-page":"5582","volume-title":"Identification of herpes virus in patients with chronic periodontitis from Northeastern Mexico","author":"Mauricio","year":"2013","journal-title":"Afr J Microbiol Res"},{"key":"key-10.4103\/1305-7456.213244-19","first-page":"15","volume-title":"Herpesvirusesin periodontal pocket and gingival tissue specimens","author":"Contreras","year":"2000","journal-title":"Oral Microbiol Immunol"},{"key":"key-10.4103\/1305-7456.213244-20","first-page":"915","volume-title":"Prevalence of herpesvirus and correlation with clinical parameters in Indian subjects with chronic periodontitis","author":"Joshi","year":"2015","journal-title":"J Contemp Dent Pract"},{"key":"key-10.4103\/1305-7456.213244-21","first-page":"1437","volume-title":"Detection of human viruses in patients with chronic periodontitis and the relationship between viruses and clinical parameters","author":"Saygun","year":"2002","journal-title":"J Periodontol"},{"key":"key-10.4103\/1305-7456.213244-22","first-page":"879","volume-title":"Herpes viruses and periodontopathic bacteria in early-onset periodontitis","author":"Kamma","year":"2001","journal-title":"J Clin Periodontol"}],"container-title":["European Journal of Dentistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.4103\/ejd.ejd_43_17.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,26]],"date-time":"2019-09-26T00:20:35Z","timestamp":1569457235000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.4103\/ejd.ejd_43_17"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7]]},"references-count":22,"journal-issue":{"issue":"03","published-online":{"date-parts":[[2019,9,25]]},"published-print":{"date-parts":[[2017,7]]}},"URL":"https:\/\/doi.org\/10.4103\/ejd.ejd_43_17","relation":{},"ISSN":["1305-7456","1305-7464"],"issn-type":[{"value":"1305-7456","type":"print"},{"value":"1305-7464","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7]]}}}