{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T13:09:39Z","timestamp":1761311379458},"reference-count":23,"publisher":"ScienceOpen","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Nuclear anomalies of different types appear in cells in response to the action of ionizing radiation after the passage of the first mitotic division. In this article, we present the results of the study of the frequency of occurrence of three types of nuclear anomalies (\u201ctailed\u201d nuclei, nucleoplasmic bridges, and dumbbell-shaped nuclei) <jats:italic>in vitro<\/jats:italic> in human lymphocytes cultured with cytochalasin B when exposed to X-rays at doses of 0.0, 0.1, 0.2, 0.4, 0.5, 0.7, 1.0, 1.5, and 2.0 Gy. To stop the cell cycle of cultured lymphocytes after the first mitotic division, a cytokinesis block was performed using cytochalasin B. Dose-dependent curves of the occurrence of lymphocytes containing \u201ctailed\u201d nuclei, nucleoplasmic bridges, or dumbbell-shaped nuclei after irradiation have been constructed. At the same time, frequencies of occurrence of chromosomal aberrations (dicentric and ring chromosomes) in the culture of lymphocytes exposed to the same radiation doses were studied. Comparison of the frequencies of occurrence of dicentric and ring chromosomes with frequencies of occurrence of nuclear anomalies allows us to conclude that these nuclear anomalies are formed as a result of chromosomal aberrations arising in lymphocytes under the action of ionizing radiation. More than that, most of the chromosomal aberrations are converted into dumbbell-shaped nuclei <jats:italic>in vitro<\/jats:italic> in the culture of lymphocytes in the cytochalasin block.<\/jats:p>","DOI":"10.4103\/genint.genint_1_18","type":"journal-article","created":{"date-parts":[[2018,5,31]],"date-time":"2018-05-31T06:08:04Z","timestamp":1527746884000},"source":"Crossref","is-referenced-by-count":4,"title":["The Frequency of Lymphocytes Containing Dumbbell-Shaped Nuclei Depends on Ionizing Radiation Dose and Correlates with Appearance of Chromosomal Aberrations"],"prefix":"10.14293","volume":"9","author":[{"given":"Viacheslav Yu.","family":"Kravtsov","sequence":"first","affiliation":[{"name":"A.M. Nikiforov Russian Center of Emergency and Radiation Medicine, EMERCOM of Russia, Saint Petersburg, Russia"},{"name":"Department of Clinical Diagnostics, S.M. Kirov Military Medical Academy, Department of Biology, Saint Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra A.","family":"Livanova","sequence":"additional","affiliation":[{"name":"Department of Clinical Diagnostics, S.M. Kirov Military Medical Academy, Department of Biology, Saint Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oleg V.","family":"Belyakov","sequence":"additional","affiliation":[{"name":"Division of Human Health, Applied Radiation Biology and Radiotherapy Section, International Atomic Energy Agency, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regina F.","family":"Fedortseva","sequence":"additional","affiliation":[{"name":"A.M. Nikiforov Russian Center of Emergency and Radiation Medicine, EMERCOM of Russia, Saint Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"5403","published-online":{"date-parts":[[2018,5,31]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.mrgentox.2004.07.012","article-title":"Frequency of micronuclei in peripheral blood lymphocytes from subjects occupationally exposed to low levels of ionizing radiation","volume":"564","author":"LJ Joseph","year":"2004","journal-title":"Mutat Res"},{"key":"ref2","first-page":"242","article-title":"Detection of nuclear anomalies in the colonic epithelium of the mouse","volume":"45","author":"DH Blakey","year":"1985","journal-title":"Cancer Res"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.mrgentox.2014.01.013","article-title":"Nuclear and cytoplasmic changes in erythrocytes of p53-deficient medaka fish (Oryzias latipes) after exposure to 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