{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T08:21:47Z","timestamp":1773735707626,"version":"3.50.1"},"reference-count":23,"publisher":"ScienceOpen","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Chromosome aberration is a biomarker that has been used as a standard tool in biological dosimetry (biodosimetry) of individuals after exposure to ionizing radiation. It is based mainly on the induction of dicentric chromosomes \u2013 one of the radiation-induced biological effects, in order to correlate them with radiation dose. In this study, a dose calibration curve for X-rays was generated by using the dicentric assay and by fitting the data to both Chromosomal Aberration Calculation Software and Dose Estimate programs to compare the output of each method. Peripheral blood samples from four nonsmoker healthy donors were irradiated with various doses ranging from 0 to 4 Gy with 250 kV or 122 keV X-rays at a dose rate of 0.17 Gy\/min. The irradiated blood was cultured, harvested, and analyzed according to the standard procedure as described by the International Atomic Energy Agency with slight modifications. The dose-response calibration data for dicentrics were fitted with the linear-quadratic model (Y<jats:sub>dic<\/jats:sub> = 0.03987D<jats:sup>2<\/jats:sup> + 0.00651D). The dose-response calibration curve obtained in this research was comparable to other estimations with similar radiation quality and dose rates. The results in this research convinced us in sustaining a biodosimetry using a dose-response calibration curve in our laboratory.<\/jats:p>","DOI":"10.4103\/genint.genint_1_19","type":"journal-article","created":{"date-parts":[[2019,7,19]],"date-time":"2019-07-19T11:30:50Z","timestamp":1563535850000},"source":"Crossref","is-referenced-by-count":13,"title":["Development of Dose-Response Calibration Curve for Dicentric Chromosome Induced by X-Rays"],"prefix":"10.14293","volume":"10","author":[{"given":"Yanti","family":"Lusiyanti","sequence":"first","affiliation":[{"name":"Center for Technology of Radiation Safety and Metrology, National Nuclear Energy Agency of Indonesia, Jakarta, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mukh","family":"Syaifudin","sequence":"additional","affiliation":[{"name":"Center for Technology of Radiation Safety and Metrology, National Nuclear Energy Agency of Indonesia, Jakarta, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tuti","family":"Budiantari","sequence":"additional","affiliation":[{"name":"Center for Technology of Radiation Safety and Metrology, National Nuclear Energy Agency of Indonesia, Jakarta, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofiati","family":"Purnami","sequence":"additional","affiliation":[{"name":"Center for Technology of Radiation Safety and Metrology, National Nuclear Energy Agency of Indonesia, Jakarta, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dwi","family":"Ramadhani","sequence":"additional","affiliation":[{"name":"Center for Technology of Radiation Safety and Metrology, National Nuclear Energy Agency of Indonesia, Jakarta, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"5403","published-online":{"date-parts":[[2019,7,19]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.mrgentox.2012.09.009","article-title":"Implementation of a dose-response curve for \u03b3-radiation in the Portuguese population by use of the chromosomal aberration assay","volume":"750","author":"V Martins","year":"2013","journal-title":"Mutat Res"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"124","DOI":"10.17146\/aij.2013.254","article-title":"Dose-response curve of chromosome aberrations in human lymphocytes induced by gamma-rays","volume":"39","author":"Y Lusiyanti","year":"2013","journal-title":"Atom Indon"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.mrgentox.2014.07.009","article-title":"Construction of dose response calibration curves for dicentrics and micronuclei for X radiation in a Serbian population","volume":"773","author":"J Pajic","year":"2014","journal-title":"Mutat Res Genet Toxicol Environ Mutagen"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"S149","DOI":"10.1269\/jrr.42.S149","article-title":"Cytogenetical dose estimation for 3 severely exposed patients in the JCO criticality accident in Tokai-Mura","volume":"42Suppl","author":"I Hayata","year":"2001","journal-title":"J Radiat Res"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1093\/jrr\/rrs065","article-title":"Cytogenetic biodosimetry for Fukushima travelers after the nuclear power plant accident: No evidence of enhanced yield of dicentrics","volume":"53","author":"JK Lee","year":"2012","journal-title":"J Radiat Res"},{"key":"ref6","volume-title":"International Atomic Energy Agency. Cytogenetic Dosimetry: Applications in Preparedness for and Response to Radiation Emergencies","year":"2011"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/0027-5107(94)00150-4","article-title":"Establishment and validation of a dose-effect curve for gamma-rays by cytogenetic analysis","volume":"326","author":"JF Barquinero","year":"1995","journal-title":"Mutat Res"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1097\/01.HP.0000346305.84577.b4","article-title":"Dose estimation software for radiation biodosimetry","volume":"98","author":"EA Ainsbury","year":"2010","journal-title":"Health Phys"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1016\/j.radmeas.2007.05.047","article-title":"The chromosome network for biodosimetry in Japan","volume":"42","author":"MA Yoshida","year":"2007","journal-title":"Radiat Meas"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"7","DOI":"10.4103\/2041-9414.197162","article-title":"Establishment of a dose-response curve for X-ray-induced micronuclei in human lymphocytes","volume":"7","author":"Y Lusiyanti","year":"2016","journal-title":"Genome Integr"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"47","DOI":"10.17146\/aij.2017.624","article-title":"Assessment of ionizing radiation induced dicentric chromosome and micronuclei in human peripheral blood lymphocyte for preliminary reconstruction of cytogenetic biodosimetry","volume":"43","author":"M Syaifudin","year":"2017","journal-title":"Atom Indon"},{"key":"ref12","volume-title":"International Atomic Energy Agency. Technical Reports Series No. 277, Absorbed Dose Determination in External Beam Radiotherapy","year":"2000"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1093\/rpd\/ncm137","article-title":"CABAS: A freely available PC program for fitting calibration curves in chromosome aberration dosimetry","volume":"124","author":"J Deperas","year":"2007","journal-title":"Radiat Prot Dosimetry"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/j.cmpb.2012.03.010","article-title":"BioDoser: Improved dose-estimation software for biological radiation dosimetry","volume":"108","author":"H Wang","year":"2012","journal-title":"Comput Methods Programs Biomed"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1590\/1414-431x20154470","article-title":"A dose-response curve for biodosimetry from a 6 MV electron linear accelerator","volume":"48","author":"MM Lemos-Pinto","year":"2015","journal-title":"Braz J Med Biol Res"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1093\/oxfordjournals.rpd.a079681","article-title":"Chromosome aberrations induced in human lymphocytes by in vitro acute X and gamma radiation","volume":"15","author":"D Lloyd","year":"1986","journal-title":"Radiat Prot Dosimetry"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S1383-5718(02)00067-0","article-title":"Chromosome aberrations induced in human lymphocytes by in vitro and in vivo X-rays","volume":"517","author":"H Schr\u00f6der","year":"2002","journal-title":"Mutat Res"},{"key":"ref18","article-title":"AFRRI's Gamma-ray, X-ray, and Fission-Neutron Calibration Curves for the Lymphocyte Dicentric Assay: Application of a Metaphase Finder System","author":"PG Prasanna","year":"2002"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1097\/HP.0b013e3181b35a53","article-title":"Establishment of an x-ray standard calibration curve by conventional dicentric analysis as prerequisite for accurate radiation dose assessment","volume":"98","author":"C Beinke","year":"2010","journal-title":"Health Phys"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1097\/HP.0b013e31829cf221","article-title":"Assessment of biodosimetry methods for a mass-casualty radiological incident: Medical response and management considerations","volume":"105","author":"JM Sullivan","year":"2013","journal-title":"Health Phys"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1097\/HP.0b013e3181b97306","article-title":"Assessment of radiation damage-the need for a multiparametric and integrative approach with the help of both clinical and biological dosimetry","volume":"98","author":"A Riecke","year":"2010","journal-title":"Health Phys"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1093\/jrr\/rrw062","article-title":"Radiosensitivity and relative biological effectiveness based on a generalized target model","volume":"58","author":"L Zhao","year":"2017","journal-title":"J Radiat Res"},{"key":"ref23","first-page":"251","article-title":"Cytogenetic damage analysed by the dicentric assay","volume":"45","author":"H Romm","year":"2009","journal-title":"Ann Ist Super Sanita"}],"container-title":["Genome Integrity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scienceopen.com\/document_file\/dbf8bab2-a36b-4191-80c4-ad068b710e30\/ScienceOpen\/dbf8bab2-a36b-4191-80c4-ad068b710e30.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scienceopen.com\/hosted-document?doi=10.4103\/genint.genint_1_19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T11:32:52Z","timestamp":1694777572000},"score":1,"resource":{"primary":{"URL":"https:\/\/scienceopen.com\/hosted-document?doi=10.4103\/genint.genint_1_19"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019]]},"references-count":23,"URL":"https:\/\/doi.org\/10.4103\/genint.genint_1_19","relation":{},"ISSN":["2041-9414"],"issn-type":[{"value":"2041-9414","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2019]]},"published":{"date-parts":[[2019]]}}}