{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T20:59:48Z","timestamp":1790888388329,"version":"4.1.0"},"reference-count":26,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,4]]},"abstract":"Abstract<\/jats:title>\n \n Background:<\/jats:title>\n \n Acinetobacter baumannii<\/jats:italic>\n is an alarming pathogen in hospital-acquired infections, particularly in the intensive care units (ICUs). Carbapenemases production and biofilm formation contribute significantly to the pathogenicity of the organism. Carbapenem resistance in\n A. baumannii<\/jats:italic>\n is primarily due to the production of carbapenemases.\n <\/jats:p>\n <\/jats:sec>\n \n Aim:<\/jats:title>\n \n The study was conducted to detect the presence of carbapenemase-encoding genes in\n A. baumannii<\/jats:italic>\n along with their ability to form biofilm. In addition, this study also investigated the association between biofilm formation and carbapenemase genes harboring\n A. baumannii<\/jats:italic>\n isolates.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n One hundred and forty isolates of\n A. baumannii<\/jats:italic>\n were collected from the various specimens. Of these, this study included 36 isolates that were carbapenem-resistant, metallo-\u03b2-lactamases (MBLs) producing, and extensively drug-resistant (XDR) obtained from ICUs. Identification and antibiotic susceptibility determination was done using VITEK 2. Further, the isolates were confirmed by detecting the\n bla<\/jats:italic>\n OXA-51 carbapenemase gene intrinsic to\n A. baumannii<\/jats:italic>\n . Polymerase chain reaction was performed to detect carbapenemase-encoding genes, and biofilm formation was examined using the tube method.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n The\n bla<\/jats:italic>\n OXA-51 gene was present in all isolates (\n n<\/jats:italic>\n = 36).\n A. baumannii<\/jats:italic>\n showed highest sensitivity against colistin (100%) and minocycline (25%). Out of 36 isolates, acquired carbapenemase encoding genes were detected in 35 (97.22%) isolates, as follows:\n bla<\/jats:italic>\n OXA-23 (35\/36, 97.22%),\n bla<\/jats:italic>\n NDM (18\/36, 50%),\n bla<\/jats:italic>\n IMP (1\/36, 2.78%), and\n bla<\/jats:italic>\n VIM (28\/36, 77.78%). In 33 (91.67%) isolates, oxacillinase (OXA) and MBL genes coexisted. Among the 36 isolates, 30 (83.33%) formed biofilm.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n \n The study highlights that colistin and minocycline are the viable antibiotics for treating carbapenem-resistant MBL-producing XDR\n A. baumannii<\/jats:italic>\n infections.\n bla<\/jats:italic>\n OXA-23 is the commonest carbapenemase gene, and\n bla<\/jats:italic>\n VIM is the most frequently found MBL gene prevalent in our hospital and contributes to carbapenem resistance in\n A. baumannii<\/jats:italic>\n . The study also showed a higher frequency of co-occurrence of multiple carbapenemase genes in single isolates, and most of these isolates formed biofilm. However, no statistically significant association was found between biofilm formation and carbapenemase genes in\n A. baumannii<\/jats:italic>\n . This study emphasizes the molecular diagnostic value of carbapenemase gene detection to reduce the resistance rate in\n A. baumannii<\/jats:italic>\n isolates.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/ijabmr.ijabmr_498_24","type":"journal-article","created":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T16:27:59Z","timestamp":1744043279000},"page":"91-97","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":7,"title":["Genetic Diversity of Carbapenemases-producing Acinetobacter baumannii Isolates at a Tertiary Teaching Hospital in Western India"],"prefix":"10.4103","volume":"15","author":[{"given":"Amitesh","family":"Datta","sequence":"first","affiliation":[{"name":"Department of Microbiology, Dr. D. Y. Patil Medical College, Hospital and Research Centre, Dr. D. Y. Patil Vidyapeeth, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nageswari","family":"Gandham","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Dr. D. Y. Patil Medical College, Hospital and Research Centre, Dr. D. Y. Patil Vidyapeeth, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chanda R.","family":"Vyawahare","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Dr. D. Y. Patil Medical College, Hospital and Research Centre, Dr. D. Y. Patil Vidyapeeth, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahzad","family":"Mirza","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Dr. D. Y. Patil Medical College, Hospital and Research Centre, Dr. D. Y. Patil Vidyapeeth, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Savita","family":"Bhatia","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Dr. D. Y. Patil Medical College, Hospital and Research Centre, Dr. D. Y. Patil Vidyapeeth, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prajakta B","family":"Shinde","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Dr. D. Y. Patil Medical College, Hospital and Research Centre, Dr. D. Y. 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