{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T19:33:22Z","timestamp":1787772802675,"version":"build-2784847793"},"reference-count":32,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      Dermatophytosis is a common global superficial mycosis caused by a group of keratinophilic moulds known as dermatophytes that invade the skin and keratinized tissues such as hair and nails of humans and animals. This study takes identification of a collection of clinical dermatophyte isolates by using partial sequencing of translation elongation factor-1\u03b1 (\n                      <jats:italic toggle=\"yes\">Tef-1\u03b1<\/jats:italic>\n                      ) gene aiming both to update the epidemiological status of dermatophytosis in Mashhad, Northeastern Iran and to corroborate the efficacy of\n                      <jats:italic toggle=\"yes\">Tef-1\u03b1<\/jats:italic>\n                      for species-level identification of dermatophytes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method:<\/jats:title>\n                    <jats:p>\n                      The demographic data related to 87 culture-positive dermatophytes isolated from patients clinically suspected to have dermatophytosis were collected. The dermatophyte isolates were subjected to a partial polymerase chain reaction (PCR)-sequencing of\n                      <jats:italic toggle=\"yes\">Tef-1\u03b1<\/jats:italic>\n                      gene by using specific pan-dermatophyte primers. The data were analysed by SeqMan software, the sequences were compared and aligned with the GenBank database and the isolates were identified.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Identification based on\n                      <jats:italic toggle=\"yes\">Tef-1\u03b1<\/jats:italic>\n                      partial sequence was successful for all isolates. The identified dermatophyte isolates in decreasing order were as\n                      <jats:italic toggle=\"yes\">Trichophyton interdigitale<\/jats:italic>\n                      19 (22%),\n                      <jats:italic toggle=\"yes\">T. tonsurans<\/jats:italic>\n                      19 (22%),\n                      <jats:italic toggle=\"yes\">T. mentagrophytes<\/jats:italic>\n                      13 (15%),\n                      <jats:italic toggle=\"yes\">T. persicum<\/jats:italic>\n                      10 (11.5%),\n                      <jats:italic toggle=\"yes\">Epidermophyton floccosum<\/jats:italic>\n                      9 (10.3%),\n                      <jats:italic toggle=\"yes\">Microsporum canis<\/jats:italic>\n                      7 (8%),\n                      <jats:italic toggle=\"yes\">T. rubrum<\/jats:italic>\n                      5 (5.7%),\n                      <jats:italic toggle=\"yes\">T. violaceum<\/jats:italic>\n                      2 (2.2%),\n                      <jats:italic toggle=\"yes\">Nannizzia fulva<\/jats:italic>\n                      2 (2.2%) and\n                      <jats:italic toggle=\"yes\">N. persicolor<\/jats:italic>\n                      1 (1.1%). The isolates have been associated with clinical forms of tinea corporis (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 38; 43.7%), tinea faciei (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 13; 15%), tinea cruris (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 12; 13.9%), tinea manuum (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 7; 8%), tinea unguium (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 7; 8%), tinea capitis (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 7; 8%) and tinea pedis (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 3; 3.4%).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      Dermatophytosis has yet remained a public health problem in Northeastern Iran, and infection with new and less frequent species, e.g.,\n                      <jats:italic toggle=\"yes\">T. persicum<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">N. fulva<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">N. persicolor<\/jats:italic>\n                      have emerged. The\n                      <jats:italic toggle=\"yes\">Tef-1\u03b1<\/jats:italic>\n                      gene partial sequencing reconfirmed the resolution power of this locus for the determination of species boundaries in dermatophytes.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ijd.ijd_573_21","type":"journal-article","created":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T17:20:31Z","timestamp":1724952031000},"page":"206","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["Update on dermatophytosis in Mashhad, Northeastern Iran, emergence of infection with Trichophyton persicum"],"prefix":"10.4103","volume":"67","author":[{"given":"Fatemeh","family":"Afsharzadeh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein","family":"Zarrinfar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdolmajid","family":"Fata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Javad","family":"Najafzadeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-43-20240829","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1007\/s12281-015-0231-4","article-title":"Dermatophytosis, trends in epidemiology and diagnostic approach","volume":"9","author":"Hayette","year":"2015","journal-title":"Curr Fungal Infect Rep"},{"key":"R2-43-20240829","first-page":"16","article-title":"Epidemiology of dermatophytosis in 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